Strain Name:
C57BL/6J-MtgxR1720Btlr/Mmmh
Stock Number:
039752-MU
Citation ID:
RRID:MMRRC_039752-MU
Other Names:
R1720 (G1), C57BL/6J-MtgxR1720Btlr
Major Collection:

Gene Information

Gene Symbol: Kmt2c [MGI:2444959] (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, HALR, E330008K23Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Itga6 [MGI:96605] (Mus musculus (mouse))
Name: integrin alpha 6
Synonyms: 5033401O05Rik, Cd49f
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Penk [MGI:104629] (Mus musculus (mouse))
Name: preproenkephalin
Synonyms: Penk, ENK, Penk1, PPA
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Grm1 [MGI:1351338] (Mus musculus (mouse))
Name: glutamate receptor, metabotropic 1
Synonyms: Grm1, 4930455H15Rik, mGluR1, Gprc1a, rcw, nmf373
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Nsd1 [MGI:1276545] (Mus musculus (mouse))
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Zdbf2 [MGI:1921134] (Mus musculus (mouse))
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Vps54 [MGI:2178798] (Mus musculus (mouse))
Name: VPS54 GARP complex subunit
Synonyms: Vps54l, mSLP8, 5330404P15Rik, wr
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,566,900 bp
  • G to A, chromosome 1 at 20,558,579 bp
  • A to T, chromosome 1 at 36,352,012 bp
  • A to T, chromosome 1 at 43,764,921 bp
  • A to G, chromosome 1 at 59,289,758 bp
  • G to A, chromosome 1 at 63,303,277 bp
  • A to G, chromosome 1 at 106,946,614 bp
  • A to G, chromosome 1 at 131,259,210 bp
  • A to G, chromosome 1 at 133,621,701 bp
  • A to T, chromosome 1 at 135,980,016 bp
  • A to T, chromosome 1 at 161,834,054 bp
  • A to G, chromosome 1 at 174,391,920 bp
  • A to G, chromosome 2 at 25,274,712 bp
  • G to A, chromosome 2 at 37,800,197 bp
  • T to C, chromosome 2 at 52,207,721 bp
  • T to A, chromosome 2 at 71,820,166 bp
  • T to C, chromosome 2 at 76,730,070 bp
  • A to T, chromosome 2 at 91,628,830 bp
  • A to T, chromosome 2 at 137,066,125 bp
  • G to T, chromosome 2 at 158,636,137 bp
  • A to T, chromosome 2 at 167,044,066 bp
  • A to T, chromosome 2 at 172,427,734 bp
  • A to T, chromosome 3 at 40,781,617 bp
  • T to C, chromosome 3 at 79,825,299 bp
  • A to T, chromosome 3 at 106,733,136 bp
  • A to G, chromosome 4 at 4,134,240 bp
  • A to T, chromosome 4 at 49,490,231 bp
  • T to C, chromosome 4 at 57,943,922 bp
  • A to T, chromosome 4 at 129,182,093 bp
  • G to T, chromosome 5 at 3,972,791 bp
  • T to G, chromosome 5 at 9,428,407 bp
  • T to C, chromosome 5 at 25,299,184 bp
  • CG to C, chromosome 5 at 101,926,525 bp
  • G to A, chromosome 5 at 115,967,897 bp
  • A to G, chromosome 5 at 118,741,995 bp
  • A to T, chromosome 5 at 150,043,099 bp
  • T to C, chromosome 6 at 43,217,560 bp
  • T to C, chromosome 7 at 27,658,816 bp
  • A to T, chromosome 7 at 29,101,870 bp
  • G to T, chromosome 7 at 44,896,991 bp
  • A to T, chromosome 7 at 82,683,721 bp
  • T to C, chromosome 7 at 86,616,456 bp
  • G to T, chromosome 7 at 98,477,230 bp
  • T to A, chromosome 7 at 103,813,876 bp
  • T to C, chromosome 7 at 105,693,828 bp
  • T to A, chromosome 7 at 127,306,927 bp
  • T to A, chromosome 8 at 72,144,754 bp
  • C to T, chromosome 9 at 38,581,289 bp
  • T to C, chromosome 9 at 53,238,071 bp
  • T to C, chromosome 9 at 57,748,179 bp
  • C to T, chromosome 9 at 106,026,472 bp
  • T to G, chromosome 9 at 106,485,239 bp
  • T to C, chromosome 9 at 109,276,102 bp
  • G to T, chromosome 10 at 10,746,794 bp
  • T to C, chromosome 10 at 45,153,199 bp
  • C to T, chromosome 10 at 76,189,269 bp
  • T to C, chromosome 10 at 89,782,586 bp
  • T to A, chromosome 10 at 107,686,294 bp
  • T to A, chromosome 10 at 127,902,258 bp
  • T to C, chromosome 11 at 6,512,490 bp
  • T to C, chromosome 11 at 21,306,519 bp
  • T to A, chromosome 11 at 45,887,410 bp
  • T to A, chromosome 11 at 75,413,981 bp
  • A to G, chromosome 11 at 97,771,609 bp
  • G to A, chromosome 11 at 98,920,820 bp
  • T to A, chromosome 11 at 102,025,756 bp
  • G to A, chromosome 11 at 115,802,782 bp
  • A to T, chromosome 11 at 116,544,607 bp
  • C to T, chromosome 11 at 120,374,581 bp
  • A to G, chromosome 12 at 84,075,881 bp
  • A to G, chromosome 13 at 42,305,350 bp
  • G to A, chromosome 13 at 55,246,898 bp
  • G to A, chromosome 13 at 112,898,899 bp
  • T to C, chromosome 14 at 33,956,909 bp
  • A to C, chromosome 14 at 52,490,594 bp
  • T to C, chromosome 14 at 66,638,278 bp
  • T to A, chromosome 15 at 34,345,274 bp
  • A to G, chromosome 15 at 77,749,366 bp
  • A to T, chromosome 15 at 78,527,440 bp
  • T to A, chromosome 15 at 79,028,995 bp
  • A to T, chromosome 15 at 99,628,769 bp
  • A to T, chromosome 16 at 29,408,928 bp
  • G to A, chromosome 16 at 33,707,179 bp
  • T to A, chromosome 16 at 49,092,782 bp
  • A to C, chromosome 17 at 17,727,008 bp
  • C to A, chromosome 18 at 5,118,264 bp
  • T to C, chromosome 18 at 6,213,427 bp
  • C to A, chromosome 18 at 10,553,848 bp
  • A to T, chromosome 18 at 22,452,435 bp
  • A to C, chromosome 18 at 35,625,851 bp
  • C to T, chromosome 18 at 53,540,200 bp
  • A to G, chromosome 18 at 60,541,622 bp
  • C to T, chromosome 18 at 73,876,735 bp
  • A to G, chromosome 19 at 4,878,951 bp
  • A to T, chromosome 19 at 33,965,666 bp
  • A to G, chromosome 19 at 36,094,427 bp
  • A to G, chromosome 19 at 44,110,672 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1720 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039752-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.