Strain Name:
C57BL/6J-MtgxR1721Btlr/Mmmh
Stock Number:
039753-MU
Citation ID:
RRID:MMRRC_039753-MU
Other Names:
R1721 (G1), C57BL/6J-MtgxR1721Btlr
Major Collection:

Gene Information

Gene Symbol: Adamts20 [MGI:2660628] (Mus musculus (mouse))
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Epha2 [MGI:95278] (Mus musculus (mouse))
Name: Eph receptor A2
Synonyms: Sek-2, Sek2, Myk2, Eck
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Pcdh9 [MGI:1306801] (Mus musculus (mouse))
Name: protocadherin 9
Synonyms: C530050I23Rik, LOC382930, A730003J17Rik, C630029H24Rik, 1500001L12Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Ccn2 [MGI:95537] (Mus musculus (mouse))
Name: cellular communication network factor 2
Synonyms: hypertrophic chondrocyte-specific gene product 24, Ctgf, Fisp12, Ccn2, Hcs24
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ptprf [MGI:102695] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: RPTP-LAR, LAR
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ncoa3 [MGI:1276535] (Mus musculus (mouse))
Name: nuclear receptor coactivator 3
Synonyms: TRAM1, Src3, 2010305B15Rik, TRAM-1, AIB1, RAC3, KAT13B, pCIP, bHLHe42
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ubr5 [MGI:1918040] (Mus musculus (mouse))
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: Edd, 4432411E13Rik, Edd1
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 86,071,845 bp
  • T to G, chromosome 1 at 89,968,580 bp
  • C to A, chromosome 1 at 95,653,669 bp
  • G to T, chromosome 1 at 134,613,181 bp
  • A to G, chromosome 2 at 4,937,809 bp
  • A to G, chromosome 2 at 25,684,016 bp
  • A to G, chromosome 2 at 49,532,105 bp
  • T to G, chromosome 2 at 119,076,334 bp
  • T to A, chromosome 2 at 160,731,920 bp
  • T to G, chromosome 2 at 166,069,301 bp
  • T to C, chromosome 2 at 166,926,411 bp
  • A to G, chromosome 3 at 92,656,011 bp
  • G to A, chromosome 3 at 94,472,869 bp
  • A to T, chromosome 3 at 95,170,788 bp
  • A to G, chromosome 3 at 103,838,598 bp
  • T to A, chromosome 3 at 107,472,176 bp
  • C to A, chromosome 4 at 11,241,684 bp
  • C to T, chromosome 4 at 21,874,086 bp
  • T to C, chromosome 4 at 32,730,997 bp
  • A to G, chromosome 4 at 59,960,598 bp
  • G to A, chromosome 4 at 61,832,370 bp
  • A to C, chromosome 4 at 118,224,899 bp
  • A to G, chromosome 4 at 126,051,394 bp
  • A to G, chromosome 4 at 141,322,652 bp
  • A to G, chromosome 4 at 155,694,898 bp
  • A to T, chromosome 4 at 156,175,173 bp
  • T to A, chromosome 5 at 30,013,492 bp
  • G to A, chromosome 5 at 36,026,748 bp
  • A to T, chromosome 5 at 77,307,581 bp
  • A to C, chromosome 6 at 42,675,338 bp
  • A to G, chromosome 6 at 113,253,732 bp
  • A to T, chromosome 6 at 138,072,847 bp
  • C to A, chromosome 7 at 6,709,901 bp
  • T to A, chromosome 7 at 27,517,523 bp
  • T to A, chromosome 7 at 45,261,176 bp
  • T to C, chromosome 7 at 45,406,921 bp
  • T to A, chromosome 7 at 98,454,061 bp
  • C to T, chromosome 7 at 140,883,517 bp
  • T to C, chromosome 8 at 64,982,739 bp
  • A to G, chromosome 8 at 69,811,232 bp
  • A to G, chromosome 9 at 15,257,367 bp
  • T to G, chromosome 9 at 56,888,743 bp
  • A to G, chromosome 9 at 75,586,042 bp
  • C to T, chromosome 9 at 107,998,803 bp
  • T to C, chromosome 9 at 114,614,999 bp
  • A to G, chromosome 9 at 118,698,306 bp
  • T to C, chromosome 9 at 119,066,014 bp
  • A to C, chromosome 10 at 24,596,797 bp
  • C to A, chromosome 10 at 49,523,746 bp
  • A to G, chromosome 10 at 79,548,830 bp
  • T to C, chromosome 10 at 98,996,888 bp
  • A to T, chromosome 10 at 123,031,097 bp
  • T to C, chromosome 11 at 51,527,889 bp
  • C to T, chromosome 11 at 58,501,939 bp
  • T to C, chromosome 11 at 69,614,972 bp
  • A to T, chromosome 11 at 74,289,300 bp
  • T to A, chromosome 11 at 86,486,270 bp
  • C to T, chromosome 11 at 106,320,820 bp
  • A to C, chromosome 11 at 116,657,545 bp
  • T to C, chromosome 12 at 79,261,799 bp
  • A to G, chromosome 13 at 9,659,368 bp
  • G to T, chromosome 13 at 15,726,297 bp
  • T to A, chromosome 13 at 38,023,333 bp
  • A to T, chromosome 14 at 20,583,440 bp
  • A to G, chromosome 14 at 26,460,218 bp
  • A to T, chromosome 14 at 54,664,538 bp
  • G to A, chromosome 14 at 93,888,035 bp
  • C to A, chromosome 15 at 10,633,228 bp
  • A to T, chromosome 15 at 38,041,846 bp
  • T to A, chromosome 15 at 55,447,462 bp
  • A to G, chromosome 15 at 94,338,459 bp
  • G to A, chromosome 15 at 96,587,135 bp
  • C to A, chromosome 16 at 35,298,424 bp
  • A to G, chromosome 16 at 88,506,556 bp
  • A to T, chromosome 17 at 3,532,882 bp
  • T to A, chromosome 17 at 14,678,810 bp
  • A to C, chromosome 17 at 22,146,203 bp
  • T to C, chromosome 17 at 24,834,047 bp
  • A to G, chromosome 17 at 35,847,826 bp
  • A to T, chromosome 17 at 36,043,153 bp
  • C to T, chromosome 17 at 90,162,404 bp
  • T to G, chromosome 18 at 52,520,911 bp
  • T to C, chromosome 18 at 54,899,249 bp
  • T to A, chromosome 18 at 61,307,204 bp
  • G to A, chromosome 19 at 3,921,271 bp
  • A to T, chromosome 19 at 29,743,598 bp
  • G to T, chromosome 19 at 40,564,838 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1721 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039753-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.