Strain Name:
C57BL/6J-MtgxR1722Btlr/Mmmh
Stock Number:
039754-MU
Citation ID:
RRID:MMRRC_039754-MU
Other Names:
R1722 (G1), C57BL/6J-MtgxR1722Btlr
Major Collection:

Gene Information

Gene Symbol: Apoa5 [MGI:1913363] (Mus musculus (mouse))
Name: apolipoprotein A-V
Synonyms: 1300007O05Rik, Apoav, RAP3
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Clasp1 [MGI:1923957] (Mus musculus (mouse))
Name: CLIP associating protein 1
Synonyms: B130045P17Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, 5730583A19Rik, CLASP1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef12 [MGI:1916882] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF) 12
Synonyms: 2310014B11Rik, LARG
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Srpk1 [MGI:106908] (Mus musculus (mouse))
Name: serine/arginine-rich protein specific kinase 1
Synonyms: SR protein-specific kinase 1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Elp3 [MGI:1921445] (Mus musculus (mouse))
Name: elongator acetyltransferase complex subunit 3
Synonyms: KAT9, 2610507P14Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Nbea [MGI:1347075] (Mus musculus (mouse))
Name: neurobeachin
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Lrrfip2 [MGI:1918518] (Mus musculus (mouse))
Name: leucine rich repeat (in FLII) interacting protein 2
Synonyms: 5133400F20Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 74,569,776 bp
  • T to G, chromosome 1 at 118,590,464 bp
  • C to T, chromosome 1 at 156,857,085 bp
  • A to G, chromosome 1 at 164,186,927 bp
  • T to C, chromosome 1 at 171,054,119 bp
  • A to G, chromosome 2 at 22,399,827 bp
  • T to C, chromosome 2 at 52,256,745 bp
  • A to C, chromosome 2 at 60,234,182 bp
  • A to G, chromosome 2 at 74,670,045 bp
  • T to C, chromosome 2 at 91,167,637 bp
  • A to T, chromosome 2 at 103,993,928 bp
  • C to T, chromosome 3 at 28,921,634 bp
  • T to C, chromosome 3 at 55,665,695 bp
  • T to G, chromosome 3 at 90,355,679 bp
  • T to A, chromosome 4 at 18,051,767 bp
  • A to T, chromosome 4 at 99,900,618 bp
  • A to T, chromosome 4 at 113,846,311 bp
  • A to T, chromosome 4 at 115,885,899 bp
  • A to C, chromosome 4 at 120,702,427 bp
  • A to T, chromosome 4 at 155,075,001 bp
  • T to C, chromosome 5 at 65,806,882 bp
  • A to T, chromosome 5 at 146,845,242 bp
  • T to A, chromosome 6 at 9,247,516 bp
  • T to A, chromosome 6 at 36,937,168 bp
  • A to G, chromosome 6 at 83,646,933 bp
  • C to T, chromosome 6 at 83,743,092 bp
  • T to A, chromosome 6 at 104,701,149 bp
  • A to G, chromosome 7 at 8,255,068 bp
  • G to A, chromosome 7 at 16,810,794 bp
  • C to A, chromosome 7 at 19,523,921 bp
  • A to T, chromosome 7 at 26,199,032 bp
  • T to C, chromosome 7 at 28,792,244 bp
  • A to G, chromosome 7 at 92,860,042 bp
  • A to C, chromosome 7 at 97,513,579 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • A to T, chromosome 7 at 131,311,644 bp
  • A to T, chromosome 7 at 142,257,862 bp
  • A to T, chromosome 8 at 25,042,960 bp
  • TGG to TG, chromosome 8 at 68,896,602 bp
  • A to T, chromosome 8 at 83,290,688 bp
  • A to T, chromosome 8 at 84,253,925 bp
  • A to G, chromosome 8 at 110,575,070 bp
  • T to C, chromosome 9 at 43,020,717 bp
  • C to T, chromosome 9 at 46,270,549 bp
  • A to G, chromosome 9 at 105,439,400 bp
  • A to G, chromosome 9 at 111,199,761 bp
  • G to T, chromosome 10 at 20,436,244 bp
  • T to A, chromosome 10 at 78,846,971 bp
  • A to T, chromosome 10 at 99,422,020 bp
  • T to C, chromosome 11 at 3,501,042 bp
  • A to C, chromosome 11 at 36,008,103 bp
  • T to A, chromosome 11 at 45,906,406 bp
  • TG to TGG, chromosome 11 at 54,546,397 bp
  • T to C, chromosome 11 at 74,334,445 bp
  • T to C, chromosome 11 at 102,154,693 bp
  • G to T, chromosome 11 at 120,382,569 bp
  • T to C, chromosome 12 at 70,248,374 bp
  • A to T, chromosome 12 at 102,812,654 bp
  • A to T, chromosome 12 at 112,636,481 bp
  • T to C, chromosome 12 at 116,059,906 bp
  • T to A, chromosome 13 at 56,479,395 bp
  • A to T, chromosome 14 at 65,551,397 bp
  • C to A, chromosome 15 at 76,697,319 bp
  • A to G, chromosome 17 at 28,599,011 bp
  • C to A, chromosome 17 at 57,211,559 bp
  • T to G, chromosome 18 at 39,775,116 bp
  • T to C, chromosome 18 at 58,048,052 bp
  • T to A, chromosome 18 at 65,157,939 bp
  • T to C, chromosome 18 at 88,973,531 bp
  • A to G, chromosome 19 at 3,964,871 bp
  • T to A, chromosome 19 at 9,010,655 bp
  • G to T, chromosome 19 at 34,248,547 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1722 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039754-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.