Strain Name:
C57BL/6J-MtgxR1722Btlr/Mmmh
Stock Number:
039754-MU
Citation ID:
RRID:MMRRC_039754-MU
Other Names:
R1722 (G1), C57BL/6J-MtgxR1722Btlr
Major Collection:

Strain Information

Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, 1300007O05Rik, Apoav
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Clasp1
Name: CLIP associating protein 1
Synonyms: CLASP1alpha, CLASP1, mCLASP1, 1700030C23Rik, 5730583A19Rik, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: 2310014B11Rik, LARG
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Srpk1
Name: serine/arginine-rich protein specific kinase 1
Synonyms: SR protein-specific kinase 1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20815
Homologene: 110962
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: KAT9, 2610507P14Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74195
VEGA: 14
Homologene: 7105
Nbea
Name: neurobeachin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Lrrfip2
Name: leucine rich repeat (in FLII) interacting protein 2
Synonyms: 5133400F20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71268
HGNC: HGNC:6703
Homologene: 22476
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: PDZ-GEF2, RA-GEF-2, C030018K18Rik, Pdzgef2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192786
Homologene: 22968
Gatad2b
Name: GATA zinc finger domain containing 2B
Synonyms: p66beta, C430014D17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229542
Homologene: 32484
Atp2c1
Name: ATPase, Ca++-sequestering
Synonyms: 1700121J11Rik, PMR1, D930003G21Rik, ATP2C1A, SPCA
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235574
Homologene: 56672
Nploc4
Name: NPL4 homolog, ubiquitin recognition factor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217365
Homologene: 5403
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: Nedd4b, 1300012C07Rik, Nedd4-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Ints4
Name: integrator complex subunit 4
Synonyms: 2610034N24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101861
Homologene: 69427
Rer1
Name: retention in endoplasmic reticulum sorting receptor 1
Synonyms: 1110060F11Rik, 5830454N22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67830
Homologene: 5468
Rsf1
Name: remodeling and spacing factor 1
Synonyms: Hbxap, p325, XAP8, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Rttn
Name: rotatin
Synonyms: 4921538A15Rik, C530033I08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 246102
VEGA: 18
Homologene: 65275
Ddias
Name: DNA damage-induced apoptosis suppressor
Synonyms: 4632434I11Rik, noxin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74041
Homologene: 51655
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: 2610040L17Rik, 9330187F13Rik, D3Bwg1534e, Ten-m2, Odz2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23964
Homologene: 22672
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC386488, LOC333789, B3bp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 333789
Homologene: 32396
Mmp16
Name: matrix metallopeptidase 16
Synonyms: MT3-MMP, Membrane type 3-MMP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17389
HGNC: HGNC:7162
Homologene: 55939
Clint1
Name: clathrin interactor 1
Synonyms: Epn4, C530049I24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216705
Homologene: 133740
Inpp5j
Name: inositol polyphosphate 5-phosphatase J
Synonyms: Pib5pa, Pipp
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170835
HGNC: HGNC:8956
Homologene: 8682
C3
Name: complement component 3
Synonyms: Plp, complement factor 3, acylation stimulating protein
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Atp6v1b1
Name: ATPase, H+ transporting, lysosomal V1 subunit B1
Synonyms: lysosomal 56/58kDa, Vpp-3, D630030L16Rik, Atp6b1, Vpp3, D630039P21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 110935
HGNC: HGNC:853
Homologene: 68198
Lpl
Name: lipoprotein lipase
Synonyms: O 1-4-5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16956
HGNC: HGNC:6677
Homologene: 200
Angptl1
Name: angiopoietin-like 1
Synonyms: ANGPT3, ANG3, ANG-3, ANG3, ARP1, 2810039D03Rik, ANGY
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72713
HGNC: HGNC:489
Homologene: 128467
Cuzd1
Name: CUB and zona pellucida-like domains 1
Synonyms: UO-44, Itmap1, UTCZP, ERG-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16433
Homologene: 7389
Zfp142
Name: zinc finger protein 142
Synonyms: 9330177B18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77264
Homologene: 3723
Zfp386
Name: zinc finger protein 386 (Kruppel-like)
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56220
Homologene: 51668
Marchf7
Name: membrane associated ring-CH-type finger 7
Synonyms: Axot, March7, Gtrgeo17
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57438
Homologene: 10753
Clec4f
Name: C-type lectin domain family 4, member f
Synonyms: Clecsf13, D18063, kupffer cell receptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 51811
Homologene: 9630
Nxph1
Name: neurexophilin 1
Synonyms: C130005L03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18231
Homologene: 8284
Trim9
Name: tripartite motif-containing 9
Synonyms: C030048G07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 94090
VEGA: 12
Homologene: 9045
Acta2
Name: actin alpha 2, smooth muscle, aorta
Synonyms: SMAalpha, SMalphaA, Actvs, 0610041G09Rik, alphaSMA, a-SMA
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11475
VEGA: 19
HGNC: HGNC:130
Homologene: 133938
4931422A03Rik
Name: RIKEN cDNA 4931422A03 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70986
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: 4930545D19Rik, hyrh, hy-3, hy3, 1700034M11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244653
Homologene: 52118
F5
Name: coagulation factor V
Synonyms: Cf-5, Cf5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Nkpd1
Name: NTPase, KAP family P-loop domain containing 1
Synonyms: 2310015G09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69547
Homologene: 18876
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Cyp2b9
Name: cytochrome P450, family 2, subfamily b, polypeptide 9
Synonyms: phenobarbitol inducible, type a, Cyp2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13094
HGNC: HGNC:2615
Homologene: 74933
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Fbn2
Name: fibrillin 2
Synonyms: Fib-2, sy, Sne
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230500
Homologene: 19952
Madd
Name: MAP-kinase activating death domain
Synonyms: Rab3 GEP, 9630059K23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242627
Homologene: 135888
Kcnq4
Name: potassium voltage-gated channel, subfamily Q, member 4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 60613
HGNC: HGNC:6298
Homologene: 78107
Ucp1
Name: uncoupling protein 1 (mitochondrial, proton carrier)
Synonyms: Slc25a7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22227
Homologene: 22524
Ahnak
Name: AHNAK nucleoprotein
Synonyms: DY6, 1110004P15Rik, 2310047C17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Adss1
Name: adenylosuccinate synthase 1
Synonyms: Adssl1, Adss
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11565
VEGA: 12
Homologene: 111992
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18459
Homologene: 56509
Pde7b
Name: phosphodiesterase 7B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 29863
HGNC: HGNC:8792
Homologene: 32194
Gm1527
Name: predicted gene 1527
Synonyms: LOC385263
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 385263
Homologene: 133976
Or3a1
Name: olfactory receptor family 3 subfamily A member 1
Synonyms: GA_x6K02T2P1NL-4467421-4466474, Olfr410, MOR255-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258702
Homologene: 68262
Aldh3b3
Name: aldehyde dehydrogenase 3 family, member B3
Synonyms: 1700055N04Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73458
HGNC: HGNC:411
Homologene: 133295
Tmem101
Name: transmembrane protein 101
Synonyms: 2610511E22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76547
Homologene: 12649
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Il9
Name: interleukin 9
Synonyms: Il-9, P40
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16198
VEGA: 13
HGNC: HGNC:6029
Homologene: 492
Fkrp
Name: fukutin related protein
Synonyms: MDC1C, A830029B19Rik, LGMD1I
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243853
Homologene: 11513
Plekha2
Name: pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2
Synonyms: TAPP2, 6430512N22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 83436
Homologene: 41822
Btbd7
Name: BTB domain containing 7
Synonyms: FUP1, E130118E17Rik, 5730507E09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238386
VEGA: 12
Homologene: 34300
Rinl
Name: Ras and Rab interactor-like
Synonyms: 9930116N10Rik, 5830482F20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320435
Homologene: 18556
Gpt
Name: glutamic pyruvic transaminase, soluble
Synonyms: ALT, Gpt1, Gpt-1, 2310022B03Rik, 1300007J06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76282
VEGA: 15
HGNC: HGNC:4552
Homologene: 134231
Or7c70
Name: olfactory receptor family 7 subfamily C member 70
Synonyms: MOR142-2_p, GA_x6K02T2QGN0-2962025-2962987, Olfr1356, MOR142-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258305
Homologene: 71958
1700111E14Rik
Name: RIKEN cDNA 1700111E14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Fcgr3
Name: Fc receptor, IgG, low affinity III
Synonyms: CD16, Fcg receptor III, FcgammaRIII
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14131
HGNC: HGNC:3616
Homologene: 47936
Hoxd13
Name: homeobox D13
Synonyms: Hox-4.8, spdh
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15433
HGNC: HGNC:5136
Homologene: 20147
Kncn
Name: kinocilin
Synonyms: Kino, L5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 654462
Homologene: 88613
Rasl11a
Name: RAS-like, family 11, member A
Synonyms: 1110065D03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68895
Homologene: 45783
Vmn2r43
Name: vomeronasal 2, receptor 43
Synonyms: EC2-V2R
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381838
Homologene: 113703
Gm2431
Name: predicted gene 2431
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 105243101
Mri1
Name: methylthioribose-1-phosphate isomerase 1
Synonyms: 2410018C20Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67873
Homologene: 7160
B530045E10Rik
Name: RIKEN cDNA B530045E10 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 118567817
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 74,569,776 bp
  • T to G, chromosome 1 at 118,590,464 bp
  • C to T, chromosome 1 at 156,857,085 bp
  • A to G, chromosome 1 at 164,186,927 bp
  • T to C, chromosome 1 at 171,054,119 bp
  • A to G, chromosome 2 at 22,399,827 bp
  • T to C, chromosome 2 at 52,256,745 bp
  • A to C, chromosome 2 at 60,234,182 bp
  • A to G, chromosome 2 at 74,670,045 bp
  • T to C, chromosome 2 at 91,167,637 bp
  • A to T, chromosome 2 at 103,993,928 bp
  • C to T, chromosome 3 at 28,921,634 bp
  • T to C, chromosome 3 at 55,665,695 bp
  • T to G, chromosome 3 at 90,355,679 bp
  • T to A, chromosome 4 at 18,051,767 bp
  • A to T, chromosome 4 at 99,900,618 bp
  • A to T, chromosome 4 at 113,846,311 bp
  • A to T, chromosome 4 at 115,885,899 bp
  • A to C, chromosome 4 at 120,702,427 bp
  • A to T, chromosome 4 at 155,075,001 bp
  • T to C, chromosome 5 at 65,806,882 bp
  • A to T, chromosome 5 at 146,845,242 bp
  • T to A, chromosome 6 at 9,247,516 bp
  • T to A, chromosome 6 at 36,937,168 bp
  • A to G, chromosome 6 at 83,646,933 bp
  • C to T, chromosome 6 at 83,743,092 bp
  • T to A, chromosome 6 at 104,701,149 bp
  • A to G, chromosome 7 at 8,255,068 bp
  • G to A, chromosome 7 at 16,810,794 bp
  • C to A, chromosome 7 at 19,523,921 bp
  • A to T, chromosome 7 at 26,199,032 bp
  • T to C, chromosome 7 at 28,792,244 bp
  • A to G, chromosome 7 at 92,860,042 bp
  • A to C, chromosome 7 at 97,513,579 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • A to T, chromosome 7 at 131,311,644 bp
  • A to T, chromosome 7 at 142,257,862 bp
  • A to T, chromosome 8 at 25,042,960 bp
  • TGG to TG, chromosome 8 at 68,896,602 bp
  • A to T, chromosome 8 at 83,290,688 bp
  • A to T, chromosome 8 at 84,253,925 bp
  • A to G, chromosome 8 at 110,575,070 bp
  • T to C, chromosome 9 at 43,020,717 bp
  • C to T, chromosome 9 at 46,270,549 bp
  • A to G, chromosome 9 at 105,439,400 bp
  • A to G, chromosome 9 at 111,199,761 bp
  • G to T, chromosome 10 at 20,436,244 bp
  • T to A, chromosome 10 at 78,846,971 bp
  • A to T, chromosome 10 at 99,422,020 bp
  • T to C, chromosome 11 at 3,501,042 bp
  • A to C, chromosome 11 at 36,008,103 bp
  • T to A, chromosome 11 at 45,906,406 bp
  • TG to TGG, chromosome 11 at 54,546,397 bp
  • T to C, chromosome 11 at 74,334,445 bp
  • T to C, chromosome 11 at 102,154,693 bp
  • G to T, chromosome 11 at 120,382,569 bp
  • T to C, chromosome 12 at 70,248,374 bp
  • A to T, chromosome 12 at 102,812,654 bp
  • A to T, chromosome 12 at 112,636,481 bp
  • T to C, chromosome 12 at 116,059,906 bp
  • T to A, chromosome 13 at 56,479,395 bp
  • A to T, chromosome 14 at 65,551,397 bp
  • C to A, chromosome 15 at 76,697,319 bp
  • A to G, chromosome 17 at 28,599,011 bp
  • C to A, chromosome 17 at 57,211,559 bp
  • T to G, chromosome 18 at 39,775,116 bp
  • T to C, chromosome 18 at 58,048,052 bp
  • T to A, chromosome 18 at 65,157,939 bp
  • T to C, chromosome 18 at 88,973,531 bp
  • A to G, chromosome 19 at 3,964,871 bp
  • T to A, chromosome 19 at 9,010,655 bp
  • G to T, chromosome 19 at 34,248,547 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1722 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039754-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.