Strain Name:
C57BL/6J-MtgxR1751Btlr/Mmmh
Stock Number:
039783-MU
Citation ID:
RRID:MMRRC_039783-MU
Other Names:
R1751 (G1), C57BL/6J-MtgxR1751Btlr
Major Collection:

Gene Information

Gene Symbol: Tln2 [MGI:1917799] (Mus musculus (mouse))
Name: talin 2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Camk2a [MGI:88256] (Mus musculus (mouse))
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Pias3 [MGI:1913126] (Mus musculus (mouse))
Name: protein inhibitor of activated STAT 3
Synonyms: Pias3L
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Cacna1g [MGI:1201678] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Gstt2 [MGI:106188] (Mus musculus (mouse))
Name: glutathione S-transferase, theta 2
Synonyms: Yrs, mGSTT2
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Grin3a [MGI:1933206] (Mus musculus (mouse))
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, A830097C19Rik, NR3A
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Pcp4 [MGI:97509] (Mus musculus (mouse))
Name: Purkinje cell protein 4
Synonyms: P16Rimb19, Pep19, Pcp-4
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 16,706,175 bp
  • A to G, chromosome 1 at 55,061,354 bp
  • A to G, chromosome 1 at 105,653,192 bp
  • A to T, chromosome 1 at 118,300,888 bp
  • C to T, chromosome 1 at 180,994,677 bp
  • A to G, chromosome 2 at 28,676,026 bp
  • T to A, chromosome 2 at 65,350,108 bp
  • G to T, chromosome 2 at 76,647,240 bp
  • AATATATATATATATATATATATATATATA to AATATATATATATATATATATATATATATATA, chromosome 2 at 111,198,021 bp
  • A to G, chromosome 2 at 127,823,945 bp
  • T to A, chromosome 2 at 174,297,894 bp
  • A to G, chromosome 2 at 174,456,896 bp
  • A to T, chromosome 3 at 45,384,177 bp
  • A to G, chromosome 3 at 88,643,953 bp
  • A to T, chromosome 3 at 93,279,913 bp
  • T to C, chromosome 3 at 96,701,403 bp
  • G to T, chromosome 3 at 136,234,614 bp
  • A to G, chromosome 3 at 136,254,937 bp
  • T to C, chromosome 3 at 159,523,287 bp
  • A to T, chromosome 4 at 49,844,423 bp
  • T to C, chromosome 4 at 98,737,449 bp
  • G to T, chromosome 4 at 107,047,415 bp
  • T to C, chromosome 4 at 118,476,176 bp
  • G to A, chromosome 4 at 126,840,724 bp
  • A to C, chromosome 4 at 129,693,183 bp
  • G to A, chromosome 4 at 132,234,887 bp
  • T to C, chromosome 5 at 88,111,452 bp
  • T to A, chromosome 5 at 112,334,432 bp
  • T to C, chromosome 5 at 135,852,172 bp
  • T to A, chromosome 5 at 136,990,176 bp
  • T to C, chromosome 5 at 144,814,575 bp
  • A to T, chromosome 6 at 5,487,053 bp
  • G to A, chromosome 6 at 106,618,410 bp
  • A to T, chromosome 6 at 124,431,999 bp
  • A to G, chromosome 6 at 125,234,705 bp
  • A to G, chromosome 6 at 134,464,568 bp
  • A to T, chromosome 6 at 136,748,775 bp
  • C to A, chromosome 7 at 24,261,896 bp
  • T to C, chromosome 7 at 26,186,675 bp
  • A to C, chromosome 7 at 116,346,236 bp
  • T to A, chromosome 8 at 43,519,911 bp
  • G to A, chromosome 8 at 123,304,281 bp
  • C to T, chromosome 9 at 67,286,514 bp
  • G to A, chromosome 9 at 75,669,340 bp
  • C to T, chromosome 9 at 102,521,377 bp
  • T to C, chromosome 9 at 111,391,479 bp
  • T to A, chromosome 9 at 123,637,100 bp
  • T to A, chromosome 10 at 50,718,376 bp
  • T to C, chromosome 10 at 62,659,666 bp
  • T to C, chromosome 10 at 67,225,690 bp
  • T to C, chromosome 10 at 75,834,264 bp
  • T to A, chromosome 10 at 79,856,546 bp
  • A to G, chromosome 10 at 94,849,815 bp
  • G to A, chromosome 11 at 69,353,901 bp
  • T to C, chromosome 11 at 94,459,802 bp
  • A to C, chromosome 11 at 100,121,100 bp
  • A to T, chromosome 11 at 109,677,838 bp
  • T to A, chromosome 11 at 119,230,696 bp
  • A to G, chromosome 12 at 16,723,438 bp
  • G to A, chromosome 12 at 99,891,343 bp
  • A to G, chromosome 13 at 22,187,271 bp
  • A to T, chromosome 13 at 40,725,137 bp
  • A to T, chromosome 13 at 49,667,408 bp
  • T to A, chromosome 13 at 56,776,143 bp
  • G to T, chromosome 13 at 67,199,257 bp
  • G to A, chromosome 14 at 49,235,884 bp
  • A to G, chromosome 14 at 66,304,168 bp
  • T to C, chromosome 14 at 76,418,102 bp
  • A to T, chromosome 14 at 103,248,405 bp
  • G to T, chromosome 15 at 11,215,528 bp
  • A to G, chromosome 15 at 79,725,557 bp
  • A to T, chromosome 15 at 98,772,675 bp
  • A to G, chromosome 16 at 23,431,374 bp
  • A to T, chromosome 16 at 30,259,667 bp
  • G to T, chromosome 16 at 31,883,929 bp
  • A to T, chromosome 16 at 48,873,628 bp
  • A to T, chromosome 16 at 52,270,714 bp
  • A to G, chromosome 16 at 74,035,024 bp
  • A to C, chromosome 16 at 91,642,949 bp
  • A to C, chromosome 16 at 96,525,489 bp
  • A to C, chromosome 17 at 12,697,441 bp
  • A to T, chromosome 17 at 20,462,524 bp
  • A to G, chromosome 17 at 23,954,803 bp
  • T to C, chromosome 17 at 25,242,639 bp
  • A to T, chromosome 17 at 37,466,901 bp
  • A to T, chromosome 17 at 45,407,805 bp
  • A to G, chromosome 17 at 56,784,754 bp
  • C to T, chromosome 18 at 9,213,643 bp
  • A to G, chromosome 18 at 34,626,704 bp
  • T to A, chromosome 18 at 60,951,281 bp
  • T to C, chromosome 18 at 78,857,398 bp
  • G to A, chromosome 19 at 4,988,183 bp
  • A to G, chromosome 19 at 8,851,771 bp
  • T to C, chromosome 19 at 26,640,380 bp
  • T to C, chromosome 19 at 33,824,991 bp
  • C to T, chromosome 19 at 40,251,904 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1751 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039783-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.