Strain Name:
C57BL/6J-MtgxR1756Btlr/Mmmh
Stock Number:
039788-MU
Citation ID:
RRID:MMRRC_039788-MU
Other Names:
R1756 (G1), C57BL/6J-MtgxR1756Btlr
Major Collection:

Gene Information

Gene Symbol: Pkd1 [MGI:97603] (Mus musculus (mouse))
Name: polycystin 1, transient receptor poteintial channel interacting
Synonyms: polycystin-1, PC1, PC-1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Th [MGI:98735] (Mus musculus (mouse))
Name: tyrosine hydroxylase
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: Cspg2, DPEAAE, heart defect, PG-M, 5430420N07Rik, hdf
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Pik3cd [MGI:1098211] (Mus musculus (mouse))
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: 2610208K16Rik, 2410099E07Rik, p110delta
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Lcor [MGI:2443930] (Mus musculus (mouse))
Name: ligand dependent nuclear receptor corepressor
Synonyms: A630025C20Rik, Mlr2, 3110023F06Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Nphs1 [MGI:1859637] (Mus musculus (mouse))
Name: nephrosis 1, nephrin
Synonyms: nephrin
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Aopep [MGI:1919311] (Mus musculus (mouse))
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 43,978,725 bp
  • A to G, chromosome 1 at 66,639,248 bp
  • A to T, chromosome 1 at 74,479,655 bp
  • C to T, chromosome 1 at 84,445,590 bp
  • C to T, chromosome 1 at 106,712,392 bp
  • A to G, chromosome 1 at 140,100,877 bp
  • C to A, chromosome 1 at 150,599,030 bp
  • A to T, chromosome 2 at 24,435,821 bp
  • C to T, chromosome 2 at 31,396,120 bp
  • T to C, chromosome 2 at 41,110,825 bp
  • A to C, chromosome 2 at 55,437,220 bp
  • T to C, chromosome 2 at 73,367,577 bp
  • A to G, chromosome 2 at 76,787,334 bp
  • A to G, chromosome 2 at 85,690,083 bp
  • G to A, chromosome 2 at 122,368,682 bp
  • T to C, chromosome 2 at 167,206,803 bp
  • T to C, chromosome 2 at 179,976,531 bp
  • T to C, chromosome 2 at 180,671,381 bp
  • A to G, chromosome 3 at 79,670,881 bp
  • A to G, chromosome 3 at 84,190,800 bp
  • C to G, chromosome 3 at 90,271,605 bp
  • A to T, chromosome 3 at 94,266,218 bp
  • A to T, chromosome 3 at 142,810,727 bp
  • T to A, chromosome 4 at 46,169,131 bp
  • C to T, chromosome 4 at 81,306,877 bp
  • T to G, chromosome 4 at 109,312,918 bp
  • C to T, chromosome 4 at 119,585,738 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • T to A, chromosome 4 at 141,415,214 bp
  • T to C, chromosome 4 at 149,658,750 bp
  • T to A, chromosome 4 at 154,925,322 bp
  • T to A, chromosome 5 at 34,466,292 bp
  • T to A, chromosome 5 at 86,420,179 bp
  • T to G, chromosome 5 at 109,218,829 bp
  • A to T, chromosome 5 at 119,845,113 bp
  • A to G, chromosome 6 at 42,590,025 bp
  • T to A, chromosome 6 at 66,612,298 bp
  • C to T, chromosome 6 at 83,675,597 bp
  • T to C, chromosome 6 at 116,626,424 bp
  • A to G, chromosome 6 at 123,267,109 bp
  • A to G, chromosome 7 at 30,461,534 bp
  • T to G, chromosome 7 at 142,229,621 bp
  • G to A, chromosome 7 at 142,898,166 bp
  • A to T, chromosome 8 at 40,825,324 bp
  • A to T, chromosome 8 at 93,306,954 bp
  • C to A, chromosome 8 at 99,421,882 bp
  • C to A, chromosome 8 at 106,264,971 bp
  • A to G, chromosome 8 at 117,592,708 bp
  • A to T, chromosome 9 at 38,430,995 bp
  • A to G, chromosome 9 at 70,410,788 bp
  • T to A, chromosome 10 at 4,357,574 bp
  • C to T, chromosome 10 at 43,910,830 bp
  • T to A, chromosome 10 at 43,986,279 bp
  • T to A, chromosome 10 at 78,586,980 bp
  • T to A, chromosome 10 at 78,747,197 bp
  • A to T, chromosome 10 at 93,048,061 bp
  • T to C, chromosome 10 at 94,750,267 bp
  • G to T, chromosome 10 at 120,094,242 bp
  • G to C, chromosome 11 at 7,208,461 bp
  • A to T, chromosome 11 at 16,992,901 bp
  • C to T, chromosome 11 at 36,063,177 bp
  • A to G, chromosome 11 at 51,733,763 bp
  • G to A, chromosome 11 at 97,034,730 bp
  • C to A, chromosome 11 at 98,241,761 bp
  • T to G, chromosome 11 at 114,750,380 bp
  • T to C, chromosome 11 at 116,607,266 bp
  • A to G, chromosome 12 at 16,538,540 bp
  • T to G, chromosome 12 at 112,763,392 bp
  • G to T, chromosome 13 at 12,396,460 bp
  • A to G, chromosome 13 at 21,674,695 bp
  • T to G, chromosome 13 at 46,531,013 bp
  • T to A, chromosome 13 at 63,068,061 bp
  • A to G, chromosome 13 at 89,691,681 bp
  • T to A, chromosome 13 at 92,575,125 bp
  • T to C, chromosome 14 at 30,932,187 bp
  • G to A, chromosome 14 at 31,547,452 bp
  • A to G, chromosome 14 at 54,665,204 bp
  • A to G, chromosome 14 at 55,739,309 bp
  • A to T, chromosome 14 at 60,244,670 bp
  • G to T, chromosome 14 at 99,086,103 bp
  • C to T, chromosome 14 at 110,750,552 bp
  • T to C, chromosome 15 at 8,338,551 bp
  • G to A, chromosome 15 at 73,025,967 bp
  • G to T, chromosome 15 at 77,393,471 bp
  • T to A, chromosome 15 at 82,617,524 bp
  • A to G, chromosome 16 at 16,342,695 bp
  • T to C, chromosome 16 at 17,650,468 bp
  • A to G, chromosome 17 at 21,690,855 bp
  • C to T, chromosome 17 at 24,594,485 bp
  • A to T, chromosome 17 at 35,619,239 bp
  • T to C, chromosome 17 at 36,286,123 bp
  • T to A, chromosome 17 at 91,088,957 bp
  • A to T, chromosome 18 at 37,854,573 bp
  • T to C, chromosome 18 at 58,572,018 bp
  • A to T, chromosome 18 at 80,127,697 bp
  • T to A, chromosome 19 at 3,268,669 bp
  • G to A, chromosome 19 at 17,123,704 bp
  • T to A, chromosome 19 at 17,395,185 bp
  • A to G, chromosome 19 at 23,893,692 bp
  • T to C, chromosome 19 at 41,559,266 bp
  • T to C, chromosome 19 at 47,622,677 bp
  • T to A, chromosome 19 at 60,825,945 bp
  • A to T, chromosome X at 144,481,226 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1756 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039788-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.