Strain Name:
C57BL/6J-MtgxR1778Btlr/Mmmh
Stock Number:
039809-MU
Citation ID:
RRID:MMRRC_039809-MU
Other Names:
R1778 (G1), C57BL/6J-MtgxR1778Btlr
Major Collection:

Gene Information

Gene Symbol: Nfat5 [MGI:1859333] (Mus musculus (mouse))
Name: nuclear factor of activated T cells 5
Synonyms: TonEBP, B130038B15Rik, OREBP, nfatz
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ippk [MGI:1922928] (Mus musculus (mouse))
Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Synonyms: 1810043M15Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Lrig2 [MGI:2443718] (Mus musculus (mouse))
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Kmt2c [MGI:2444959] (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, HALR, E330008K23Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Sim1 [MGI:98306] (Mus musculus (mouse))
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Esco2 [MGI:1919238] (Mus musculus (mouse))
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: 2410004I17Rik, D030072L07Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 67,006,857 bp
  • A to T, chromosome 1 at 92,608,109 bp
  • G to A, chromosome 1 at 92,620,620 bp
  • A to G, chromosome 1 at 135,908,994 bp
  • A to G, chromosome 1 at 149,902,445 bp
  • A to G, chromosome 1 at 178,325,241 bp
  • A to T, chromosome 1 at 179,753,015 bp
  • A to G, chromosome 1 at 194,810,970 bp
  • T to A, chromosome 2 at 19,383,303 bp
  • A to G, chromosome 2 at 28,712,321 bp
  • A to G, chromosome 2 at 35,354,983 bp
  • T to C, chromosome 2 at 60,006,136 bp
  • T to A, chromosome 2 at 91,099,522 bp
  • T to C, chromosome 2 at 91,766,711 bp
  • A to C, chromosome 2 at 127,803,122 bp
  • A to T, chromosome 2 at 132,058,189 bp
  • C to A, chromosome 2 at 154,163,726 bp
  • G to A, chromosome 2 at 180,195,481 bp
  • G to A, chromosome 3 at 36,465,331 bp
  • A to T, chromosome 3 at 49,755,634 bp
  • A to G, chromosome 3 at 60,017,450 bp
  • A to G, chromosome 3 at 79,660,057 bp
  • T to A, chromosome 3 at 87,385,319 bp
  • A to G, chromosome 3 at 90,189,486 bp
  • A to G, chromosome 3 at 104,467,366 bp
  • T to C, chromosome 3 at 122,590,147 bp
  • A to G, chromosome 3 at 159,622,848 bp
  • T to A, chromosome 4 at 4,058,814 bp
  • G to T, chromosome 4 at 19,627,892 bp
  • A to G, chromosome 4 at 48,052,211 bp
  • A to T, chromosome 4 at 56,903,968 bp
  • A to G, chromosome 4 at 58,984,865 bp
  • T to A, chromosome 4 at 91,253,478 bp
  • A to C, chromosome 4 at 96,199,390 bp
  • G to A, chromosome 4 at 101,773,010 bp
  • T to C, chromosome 4 at 154,287,986 bp
  • A to T, chromosome 4 at 154,897,357 bp
  • A to G, chromosome 5 at 23,492,364 bp
  • T to C, chromosome 5 at 25,372,974 bp
  • C to A, chromosome 5 at 129,845,068 bp
  • T to C, chromosome 5 at 138,262,477 bp
  • A to T, chromosome 6 at 7,983,124 bp
  • T to A, chromosome 6 at 38,409,476 bp
  • A to G, chromosome 6 at 71,577,807 bp
  • A to G, chromosome 6 at 78,383,286 bp
  • T to A, chromosome 6 at 121,334,986 bp
  • T to C, chromosome 7 at 29,560,706 bp
  • A to G, chromosome 7 at 30,231,612 bp
  • A to G, chromosome 7 at 30,297,436 bp
  • A to G, chromosome 7 at 44,462,399 bp
  • G to A, chromosome 7 at 45,147,308 bp
  • A to T, chromosome 7 at 45,211,395 bp
  • A to T, chromosome 7 at 86,761,699 bp
  • A to T, chromosome 7 at 100,300,691 bp
  • A to T, chromosome 7 at 105,024,163 bp
  • A to G, chromosome 7 at 120,078,402 bp
  • T to A, chromosome 7 at 141,232,456 bp
  • A to C, chromosome 8 at 40,796,661 bp
  • A to G, chromosome 8 at 54,690,214 bp
  • A to G, chromosome 8 at 61,974,176 bp
  • T to C, chromosome 8 at 83,930,037 bp
  • C to A, chromosome 8 at 105,014,607 bp
  • C to A, chromosome 8 at 105,329,677 bp
  • C to T, chromosome 8 at 107,361,789 bp
  • C to T, chromosome 9 at 42,343,631 bp
  • T to C, chromosome 9 at 69,415,648 bp
  • A to G, chromosome 9 at 70,476,112 bp
  • T to A, chromosome 9 at 79,604,585 bp
  • G to A, chromosome 9 at 110,980,840 bp
  • C to A, chromosome 10 at 12,436,364 bp
  • G to T, chromosome 10 at 23,899,517 bp
  • C to A, chromosome 10 at 50,981,553 bp
  • C to T, chromosome 10 at 63,054,371 bp
  • G to T, chromosome 10 at 77,982,944 bp
  • T to C, chromosome 10 at 84,374,874 bp
  • A to G, chromosome 10 at 116,961,939 bp
  • A to G, chromosome 10 at 126,010,075 bp
  • A to G, chromosome 10 at 127,884,721 bp
  • T to C, chromosome 10 at 129,705,705 bp
  • T to A, chromosome 11 at 4,135,873 bp
  • C to A, chromosome 11 at 60,478,412 bp
  • C to T, chromosome 11 at 110,130,716 bp
  • T to A, chromosome 11 at 116,109,820 bp
  • G to A, chromosome 12 at 16,690,894 bp
  • C to T, chromosome 12 at 25,013,446 bp
  • C to T, chromosome 12 at 51,753,807 bp
  • A to G, chromosome 12 at 85,171,767 bp
  • T to A, chromosome 12 at 102,561,700 bp
  • G to A, chromosome 13 at 27,659,271 bp
  • T to C, chromosome 13 at 49,436,813 bp
  • T to C, chromosome 14 at 50,829,622 bp
  • A to G, chromosome 14 at 56,522,399 bp
  • G to T, chromosome 14 at 61,605,619 bp
  • A to G, chromosome 14 at 65,831,262 bp
  • A to T, chromosome 15 at 5,235,095 bp
  • A to C, chromosome 15 at 8,319,488 bp
  • A to G, chromosome 15 at 83,610,204 bp
  • C to T, chromosome 15 at 83,791,990 bp
  • A to G, chromosome 15 at 98,912,476 bp
  • T to C, chromosome 15 at 102,339,431 bp
  • T to A, chromosome 15 at 103,487,097 bp
  • T to C, chromosome 16 at 5,010,505 bp
  • T to C, chromosome 16 at 32,794,141 bp
  • T to C, chromosome 16 at 97,950,585 bp
  • T to C, chromosome 17 at 20,114,015 bp
  • G to A, chromosome 17 at 28,220,186 bp
  • A to G, chromosome 17 at 33,302,814 bp
  • A to G, chromosome 17 at 34,683,574 bp
  • A to T, chromosome 18 at 7,127,388 bp
  • T to C, chromosome 18 at 36,756,818 bp
  • A to T, chromosome 18 at 60,270,948 bp
  • G to T, chromosome 18 at 86,713,147 bp
  • T to A, chromosome 19 at 12,241,455 bp
  • T to A, chromosome 19 at 34,594,193 bp
  • A to G, chromosome 19 at 38,780,790 bp
  • A to G, chromosome 19 at 39,038,085 bp
  • C to T, chromosome 19 at 46,331,640 bp
  • C to T, chromosome 19 at 56,916,206 bp
  • C to T, chromosome Y at 1,011,287 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1778 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039809-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.