Strain Name:
C57BL/6J-MtgxR1812Btlr/Mmmh
Stock Number:
039840-MU
Citation ID:
RRID:MMRRC_039840-MU
Other Names:
R1812 (G1), C57BL/6J-MtgxR1812Btlr
Major Collection:

Gene Information

Gene Symbol: Itpk1 [MGI:2446159] (Mus musculus (mouse))
Name: inositol 1,3,4-triphosphate 5/6 kinase
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Cdkn1a [MGI:104556] (Mus musculus (mouse))
Name: cyclin-dependent kinase inhibitor 1A (P21)
Synonyms: CAP20, CIP1, p21Cip1, p21WAF1/CIP1, p21WAF, SDI1, Cdkn1, mda6, Waf1, P21, CDKI
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Kif5a [MGI:109564] (Mus musculus (mouse))
Name: kinesin family member 5A
Synonyms: Kns, Khc, Kif5, D10Bwg0738e
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, Ptpz, phosphacan, Rptpbeta, Ptprz, RPTPz, PTPbeta, PTPzeta
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Crhr1 [MGI:88498] (Mus musculus (mouse))
Name: corticotropin releasing hormone receptor 1
Synonyms: CRF1R, CRF 1 receptor, CRFR1, CRF-R1alpha
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Epb41l1 [MGI:103010] (Mus musculus (mouse))
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 65,258,923 bp
  • A to T, chromosome 1 at 91,196,857 bp
  • C to T, chromosome 1 at 129,758,610 bp
  • T to A, chromosome 1 at 134,324,573 bp
  • T to A, chromosome 2 at 34,725,064 bp
  • G to A, chromosome 2 at 35,149,469 bp
  • G to A, chromosome 2 at 59,791,679 bp
  • A to G, chromosome 2 at 87,693,103 bp
  • T to G, chromosome 2 at 118,632,421 bp
  • A to G, chromosome 2 at 137,068,195 bp
  • A to G, chromosome 2 at 156,046,938 bp
  • T to C, chromosome 2 at 156,496,511 bp
  • T to C, chromosome 3 at 60,025,077 bp
  • G to GGG, chromosome 3 at 96,580,911 bp
  • CGGG to CGGGGG, chromosome 3 at 96,580,912 bp
  • A to T, chromosome 4 at 11,612,770 bp
  • G to T, chromosome 4 at 43,770,230 bp
  • T to A, chromosome 4 at 123,432,024 bp
  • T to C, chromosome 4 at 132,768,685 bp
  • T to A, chromosome 4 at 136,542,331 bp
  • T to A, chromosome 4 at 148,498,083 bp
  • G to A, chromosome 5 at 8,928,578 bp
  • T to A, chromosome 5 at 29,206,260 bp
  • G to A, chromosome 5 at 37,466,758 bp
  • A to C, chromosome 5 at 102,913,262 bp
  • A to T, chromosome 5 at 146,102,811 bp
  • T to A, chromosome 6 at 22,959,712 bp
  • G to A, chromosome 6 at 38,698,163 bp
  • A to G, chromosome 6 at 40,409,431 bp
  • A to G, chromosome 6 at 83,192,518 bp
  • T to G, chromosome 6 at 132,657,601 bp
  • T to A, chromosome 7 at 31,037,930 bp
  • T to A, chromosome 7 at 43,777,755 bp
  • T to A, chromosome 7 at 45,076,574 bp
  • C to G, chromosome 7 at 45,282,947 bp
  • T to G, chromosome 7 at 66,171,817 bp
  • A to G, chromosome 7 at 86,920,787 bp
  • G to A, chromosome 7 at 96,895,940 bp
  • A to T, chromosome 7 at 102,559,671 bp
  • A to T, chromosome 7 at 102,614,370 bp
  • T to C, chromosome 7 at 102,888,078 bp
  • A to T, chromosome 7 at 116,417,664 bp
  • C to A, chromosome 8 at 4,103,612 bp
  • A to G, chromosome 8 at 45,036,803 bp
  • CAGAG to CAG, chromosome 8 at 107,170,388 bp
  • G to A, chromosome 9 at 45,751,286 bp
  • G to A, chromosome 9 at 57,257,457 bp
  • C to T, chromosome 9 at 60,056,937 bp
  • A to G, chromosome 9 at 105,928,054 bp
  • C to T, chromosome 9 at 110,550,102 bp
  • G to T, chromosome 9 at 119,780,865 bp
  • A to G, chromosome 10 at 14,760,539 bp
  • A to G, chromosome 10 at 79,379,212 bp
  • C to T, chromosome 10 at 93,038,982 bp
  • T to A, chromosome 10 at 120,089,566 bp
  • A to T, chromosome 10 at 127,242,010 bp
  • C to T, chromosome 11 at 3,685,831 bp
  • A to G, chromosome 11 at 60,759,362 bp
  • A to G, chromosome 11 at 80,110,140 bp
  • A to G, chromosome 11 at 80,133,047 bp
  • T to C, chromosome 11 at 98,632,393 bp
  • T to C, chromosome 11 at 104,169,147 bp
  • T to C, chromosome 11 at 105,886,386 bp
  • T to C, chromosome 12 at 21,361,767 bp
  • C to T, chromosome 12 at 98,275,742 bp
  • T to A, chromosome 12 at 102,574,058 bp
  • C to T, chromosome 12 at 110,662,900 bp
  • C to A, chromosome 13 at 11,560,586 bp
  • C to T, chromosome 13 at 14,195,429 bp
  • A to G, chromosome 13 at 55,535,954 bp
  • G to A, chromosome 14 at 19,402,395 bp
  • T to C, chromosome 15 at 8,079,179 bp
  • G to A, chromosome 15 at 9,679,349 bp
  • T to C, chromosome 15 at 47,644,128 bp
  • T to C, chromosome 15 at 90,971,766 bp
  • C to A, chromosome 15 at 100,198,938 bp
  • T to G, chromosome 15 at 100,211,974 bp
  • A to G, chromosome 16 at 16,917,787 bp
  • T to A, chromosome 17 at 5,337,029 bp
  • A to G, chromosome 17 at 25,845,705 bp
  • T to C, chromosome 17 at 29,098,565 bp
  • A to G, chromosome 17 at 34,028,815 bp
  • A to T, chromosome 18 at 5,097,545 bp
  • G to T, chromosome 18 at 37,891,018 bp
  • T to C, chromosome 19 at 44,998,117 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1812 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039840-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.