Strain Name:
C57BL/6J-MtgxR1816Btlr/Mmmh
Stock Number:
039844-MU
Citation ID:
RRID:MMRRC_039844-MU
Other Names:
R1816 (G1), C57BL/6J-MtgxR1816Btlr
Major Collection:

Gene Information

Gene Symbol: Myo10 [MGI:107716] (Mus musculus (mouse))
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Stx8 [MGI:1890156] (Mus musculus (mouse))
Name: syntaxin 8
Synonyms: 4930571E13Rik, 1110002H11Rik, 0610007H08Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dicer1 [MGI:2177178] (Mus musculus (mouse))
Name: dicer 1, ribonuclease type III
Synonyms: D12Ertd7e, Dicer1, 1110006F08Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Camkk2 [MGI:2444812] (Mus musculus (mouse))
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Itga6 [MGI:96605] (Mus musculus (mouse))
Name: integrin alpha 6
Synonyms: 5033401O05Rik, Cd49f
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Tfap2b [MGI:104672] (Mus musculus (mouse))
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), Tcfap2b, E130018K07Rik
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Tnpo3 [MGI:1196412] (Mus musculus (mouse))
Name: transportin 3
Synonyms: 5730544L10Rik, D6Ertd313e, C430013M08Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 19,209,212 bp
  • A to G, chromosome 1 at 53,631,742 bp
  • A to G, chromosome 1 at 54,492,057 bp
  • A to G, chromosome 1 at 59,582,399 bp
  • G to T, chromosome 1 at 116,428,888 bp
  • C to T, chromosome 1 at 133,101,370 bp
  • T to A, chromosome 1 at 140,147,646 bp
  • A to G, chromosome 1 at 171,872,750 bp
  • T to A, chromosome 1 at 183,098,575 bp
  • T to C, chromosome 2 at 71,840,809 bp
  • G to T, chromosome 2 at 85,590,925 bp
  • A to T, chromosome 2 at 86,553,667 bp
  • T to G, chromosome 2 at 88,402,599 bp
  • C to T, chromosome 2 at 143,841,679 bp
  • A to T, chromosome 2 at 155,025,629 bp
  • C to T, chromosome 2 at 156,035,199 bp
  • G to T, chromosome 2 at 158,768,150 bp
  • T to C, chromosome 3 at 19,968,220 bp
  • T to C, chromosome 3 at 83,838,209 bp
  • T to C, chromosome 3 at 89,276,387 bp
  • T to C, chromosome 3 at 90,053,091 bp
  • T to C, chromosome 3 at 130,634,562 bp
  • G to T, chromosome 3 at 146,481,207 bp
  • G to T, chromosome 4 at 55,530,977 bp
  • C to A, chromosome 4 at 63,565,710 bp
  • A to G, chromosome 5 at 3,734,028 bp
  • T to A, chromosome 5 at 31,245,813 bp
  • C to T, chromosome 5 at 34,803,740 bp
  • C to A, chromosome 5 at 35,700,584 bp
  • T to C, chromosome 5 at 52,750,746 bp
  • A to G, chromosome 5 at 87,889,470 bp
  • A to G, chromosome 5 at 104,517,834 bp
  • A to G, chromosome 5 at 110,787,831 bp
  • T to G, chromosome 5 at 121,501,725 bp
  • A to G, chromosome 5 at 122,734,180 bp
  • A to G, chromosome 5 at 138,260,341 bp
  • T to C, chromosome 6 at 29,557,017 bp
  • G to T, chromosome 6 at 90,072,803 bp
  • A to T, chromosome 6 at 111,495,791 bp
  • C to A, chromosome 6 at 124,230,371 bp
  • T to C, chromosome 7 at 4,811,555 bp
  • T to A, chromosome 7 at 18,071,765 bp
  • T to A, chromosome 7 at 40,994,798 bp
  • G to A, chromosome 7 at 103,840,378 bp
  • A to T, chromosome 7 at 106,873,488 bp
  • T to C, chromosome 7 at 108,630,157 bp
  • T to C, chromosome 7 at 135,707,387 bp
  • A to T, chromosome 8 at 4,629,147 bp
  • T to A, chromosome 8 at 31,738,958 bp
  • T to A, chromosome 8 at 41,309,537 bp
  • T to A, chromosome 8 at 105,709,859 bp
  • A to G, chromosome 9 at 24,725,804 bp
  • T to C, chromosome 9 at 95,866,694 bp
  • G to A, chromosome 10 at 121,925,971 bp
  • G to A, chromosome 11 at 32,207,565 bp
  • G to A, chromosome 11 at 54,694,488 bp
  • T to A, chromosome 11 at 68,011,326 bp
  • T to C, chromosome 11 at 72,058,376 bp
  • T to C, chromosome 11 at 73,847,199 bp
  • A to G, chromosome 11 at 100,703,152 bp
  • A to G, chromosome 11 at 102,351,230 bp
  • A to T, chromosome 11 at 107,060,579 bp
  • T to C, chromosome 12 at 104,722,151 bp
  • T to C, chromosome 14 at 31,157,465 bp
  • A to T, chromosome 14 at 57,890,143 bp
  • T to C, chromosome 15 at 25,800,200 bp
  • T to C, chromosome 15 at 44,528,239 bp
  • T to C, chromosome 15 at 91,806,728 bp
  • T to G, chromosome 16 at 36,808,009 bp
  • A to G, chromosome 16 at 37,826,724 bp
  • C to A, chromosome 17 at 14,670,713 bp
  • T to A, chromosome 17 at 14,670,714 bp
  • A to G, chromosome 17 at 18,166,677 bp
  • T to A, chromosome 17 at 27,986,573 bp
  • C to A, chromosome 17 at 56,808,305 bp
  • G to A, chromosome 17 at 59,010,136 bp
  • G to A, chromosome 19 at 6,542,653 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1816 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039844-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.