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Strain Name:
C57BL/6J-MtgxR1819Btlr/Mmmh
Stock Number:
039847-MU
Citation ID:
RRID:MMRRC_039847-MU
Other Names:
R1819 (G1), C57BL/6J-MtgxR1819Btlr
Major Collection:

Strain Information

Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643
Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Pvalb
Name: parvalbumin
Synonyms: PV, Pva, Parv
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19293
HGNC: HGNC:9704
Homologene: 2137
Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Tagln
Name: transgelin
Synonyms: Sm22a, SM-22 alpha, SM22, SM22alpha
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21345
VEGA: 9
Homologene: 2398
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,531,527 bp
  • A to C, chromosome 1 at 53,559,148 bp
  • A to G, chromosome 1 at 57,394,036 bp
  • G to T, chromosome 1 at 73,916,476 bp
  • A to G, chromosome 1 at 97,785,226 bp
  • A to T, chromosome 1 at 128,017,556 bp
  • T to G, chromosome 1 at 128,259,018 bp
  • A to G, chromosome 1 at 136,432,507 bp
  • A to G, chromosome 1 at 155,225,829 bp
  • C to T, chromosome 1 at 164,282,478 bp
  • T to A, chromosome 1 at 166,638,219 bp
  • T to C, chromosome 1 at 179,705,754 bp
  • T to A, chromosome 1 at 182,472,597 bp
  • T to A, chromosome 1 at 182,598,826 bp
  • T to A, chromosome 1 at 194,790,186 bp
  • A to G, chromosome 2 at 29,074,784 bp
  • C to T, chromosome 2 at 30,110,218 bp
  • C to T, chromosome 2 at 36,052,975 bp
  • T to G, chromosome 3 at 51,542,639 bp
  • T to C, chromosome 3 at 59,097,920 bp
  • A to G, chromosome 3 at 86,542,634 bp
  • G to T, chromosome 4 at 43,512,439 bp
  • A to G, chromosome 4 at 45,926,195 bp
  • A to G, chromosome 4 at 83,458,163 bp
  • A to G, chromosome 4 at 84,291,874 bp
  • A to T, chromosome 4 at 96,297,739 bp
  • T to C, chromosome 4 at 126,323,736 bp
  • G to T, chromosome 4 at 135,686,523 bp
  • A to C, chromosome 5 at 65,166,963 bp
  • A to T, chromosome 5 at 65,212,891 bp
  • A to C, chromosome 5 at 120,536,227 bp
  • G to A, chromosome 5 at 120,808,735 bp
  • A to T, chromosome 5 at 137,323,032 bp
  • A to G, chromosome 6 at 120,731,267 bp
  • T to C, chromosome 6 at 124,854,932 bp
  • T to C, chromosome 6 at 142,461,186 bp
  • T to A, chromosome 7 at 25,463,860 bp
  • C to T, chromosome 7 at 25,615,290 bp
  • G to T, chromosome 7 at 28,085,283 bp
  • G to C, chromosome 7 at 45,048,697 bp
  • C to T, chromosome 7 at 46,099,156 bp
  • T to A, chromosome 7 at 75,608,705 bp
  • C to T, chromosome 7 at 104,975,398 bp
  • G to A, chromosome 7 at 127,883,120 bp
  • A to G, chromosome 7 at 135,668,993 bp
  • G to A, chromosome 7 at 142,438,234 bp
  • T to A, chromosome 8 at 4,521,232 bp
  • G to A, chromosome 8 at 45,315,636 bp
  • A to T, chromosome 8 at 54,690,124 bp
  • T to A, chromosome 8 at 106,051,848 bp
  • T to A, chromosome 9 at 27,311,593 bp
  • A to T, chromosome 9 at 45,857,162 bp
  • A to G, chromosome 9 at 45,930,840 bp
  • G to T, chromosome 9 at 49,107,164 bp
  • T to C, chromosome 9 at 59,943,437 bp
  • G to A, chromosome 9 at 72,109,717 bp
  • G to A, chromosome 9 at 104,114,539 bp
  • A to T, chromosome 10 at 42,197,611 bp
  • A to G, chromosome 10 at 51,496,028 bp
  • T to C, chromosome 10 at 80,586,636 bp
  • C to T, chromosome 10 at 83,550,884 bp
  • A to C, chromosome 10 at 128,853,173 bp
  • T to C, chromosome 11 at 73,906,679 bp
  • G to T, chromosome 11 at 100,415,889 bp
  • T to C, chromosome 11 at 101,329,184 bp
  • T to C, chromosome 11 at 109,981,056 bp
  • A to T, chromosome 11 at 115,276,104 bp
  • C to T, chromosome 12 at 54,783,474 bp
  • T to C, chromosome 12 at 57,694,500 bp
  • T to C, chromosome 12 at 69,963,300 bp
  • T to C, chromosome 12 at 76,008,258 bp
  • T to A, chromosome 12 at 100,878,403 bp
  • C to T, chromosome 13 at 15,725,792 bp
  • T to G, chromosome 13 at 110,084,135 bp
  • A to G, chromosome 14 at 24,298,013 bp
  • A to G, chromosome 14 at 43,232,568 bp
  • G to A, chromosome 14 at 57,455,519 bp
  • G to T, chromosome 15 at 18,991,965 bp
  • T to A, chromosome 15 at 28,246,400 bp
  • T to C, chromosome 15 at 47,753,735 bp
  • G to T, chromosome 15 at 75,045,613 bp
  • C to T, chromosome 15 at 76,179,906 bp
  • A to C, chromosome 15 at 78,202,584 bp
  • T to C, chromosome 15 at 80,882,935 bp
  • T to A, chromosome 15 at 99,819,936 bp
  • T to C, chromosome 16 at 32,826,914 bp
  • A to G, chromosome 17 at 13,850,848 bp
  • T to C, chromosome 17 at 24,110,904 bp
  • T to C, chromosome 17 at 24,681,130 bp
  • A to G, chromosome 17 at 24,687,722 bp
  • G to T, chromosome 17 at 25,473,811 bp
  • G to A, chromosome 17 at 26,438,700 bp
  • A to T, chromosome 17 at 28,684,834 bp
  • C to A, chromosome 17 at 43,310,033 bp
  • C to T, chromosome 17 at 43,626,551 bp
  • T to C, chromosome 17 at 78,791,511 bp
  • A to T, chromosome 18 at 22,522,376 bp
  • A to G, chromosome 18 at 52,638,800 bp
  • A to G, chromosome X at 21,741,047 bp
  • A to G, chromosome X at 36,358,708 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1819 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039847-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.