Strain Name:
C57BL/6J-MtgxR1820Btlr/Mmmh
Stock Number:
039848-MU
Citation ID:
RRID:MMRRC_039848-MU
Other Names:
R1820 (G1), C57BL/6J-MtgxR1820Btlr
Major Collection:

Strain Information

Itprip
Name: inositol 1,4,5-triphosphate receptor interacting protein
Synonyms: DANGER
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 414801
VEGA: 19
Homologene: 62157
Gabrg1
Name: gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Kdm5b
Name: lysine (K)-specific demethylase 5B
Synonyms: 2010009J12Rik, 2210016I17Rik, D1Ertd202e, Rb-Bp2, Jarid1b, PLU-1, Plu1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75605
Homologene: 48448
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 9530077A04Rik, 1810033J14Rik, Mll5, D230038D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69188
Homologene: 18822
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: 2310057J16Rik, Nezha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69697
Homologene: 18966
Ambra1
Name: autophagy/beclin 1 regulator 1
Synonyms: 2310079H06Rik, D030051N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228361
Homologene: 18204
Luc7l2
Name: LUC7-like 2 (S. cerevisiae)
Synonyms: CGI-59, 4930471C18Rik, CGI-74
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 192196
Homologene: 56737
Arhgap35
Name: Rho GTPase activating protein 35
Synonyms: Grlf1, p190A, p190RhoGAP, 6430596G11Rik, P190 RhoGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232906
HGNC: HGNC:4591
Homologene: 35136
Ptprg
Name: protein tyrosine phosphatase receptor type G
Synonyms: RPTPgamma, 5430405N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Sp1
Name: trans-acting transcription factor 1
Synonyms: Sp1-1, 1110003E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20683
Homologene: 8276
Acbd3
Name: acyl-Coenzyme A binding domain containing 3
Synonyms: Pap7, 8430407O11Rik, D1Ertd10e, Gocap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 170760
Homologene: 11227
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: MICAL-3, C130040D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194401
Homologene: 85288
Myo9b
Name: myosin IXb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Lrrc28
Name: leucine rich repeat containing 28
Synonyms: 2210012C09Rik, 1300004K21Rik, 2310058O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67867
Homologene: 16944
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Kif23
Name: kinesin family member 23
Synonyms: CHO1, 3110001D19Rik, MKLP-1, MKLP1, C87313, Knsl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71819
VEGA: 9
HGNC: HGNC:6392
Homologene: 11491
Scimp
Name: SLP adaptor and CSK interacting membrane protein
Synonyms: A430084P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327957
Homologene: 52602
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Stau2
Name: staufen double-stranded RNA binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 29819
Homologene: 8666
Arl16
Name: ADP-ribosylation factor-like 16
Synonyms: 2600005N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70317
Homologene: 53461
Nup58
Name: nucleoporin 58
Synonyms: Nupl1, 1700017F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Cwf19l1
Name: CWF19-like 1, cell cycle control (S. pombe)
Synonyms: 2610528C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72502
VEGA: 19
Homologene: 41244
2700049A03Rik
Name: RIKEN cDNA 2700049A03 gene
Synonyms: Ta3, talpid3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76967
Homologene: 8839
Sfrp2
Name: secreted frizzled-related protein 2
Synonyms: Sdf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20319
Homologene: 56438
Adarb1
Name: adenosine deaminase, RNA-specific, B1
Synonyms: RED1, D10Bwg0447e, 1700057H01Rik, ADAR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 110532
HGNC: HGNC:226
Homologene: 8280
Shf
Name: Src homology 2 domain containing F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 435684
Homologene: 44524
Spink5
Name: serine peptidase inhibitor, Kazal type 5
Synonyms: 2310065D10Rik, LEKT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72432
Homologene: 4987
Ank1
Name: ankyrin 1, erythroid
Synonyms: pale, Ank-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546144
Homologene: 52326
Rnf157
Name: ring finger protein 157
Synonyms: 2610036E23Rik, A130073L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217340
Homologene: 28235
Fhip1a
Name: FHF complex subunit HOOK interacting protein 1A
Synonyms: 9930021J17Rik, Fam160a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229488
Homologene: 85149
Psmd5
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Synonyms: 1500032A03Rik, S5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66998
HGNC: HGNC:9563
Homologene: 37999
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: Si-s, 2010204N08Rik, sucrase-isomaltase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Cplane1
Name: ciliogenesis and planar polarity effector 1
Synonyms: b2b012Clo, 2410089E03Rik, Hug, Jbts17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 73692
Homologene: 11315
Nynrin
Name: NYN domain and retroviral integrase containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 277154
VEGA: 14
Homologene: 19483
Plekhs1
Name: pleckstrin homology domain containing, family S member 1
Synonyms: 9930023K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226245
VEGA: 19
Homologene: 11770
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: 6030457M03Rik, CTF18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214901
Homologene: 32532
Tdrd5
Name: tudor domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 214575
Homologene: 18312
Pnp2
Name: purine-nucleoside phosphorylase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 667034
HGNC: HGNC:7892
Homologene: 227
Scd3
Name: stearoyl-coenzyme A desaturase 3
Synonyms: 4930513N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 30049
VEGA: 19
Homologene: 137370
Rfx6
Name: regulatory factor X, 6
Synonyms: Rfxdc1, 4930572O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320995
Homologene: 18318
Grik5
Name: glutamate receptor, ionotropic, kainate 5 (gamma 2)
Synonyms: GluRgamma2, KA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14809
HGNC: HGNC:4583
Homologene: 1578
St8sia3
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Synonyms: ST8SiaIII, Siat8c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20451
Homologene: 8285
Adhfe1
Name: alcohol dehydrogenase, iron containing, 1
Synonyms: 6330565B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76187
Homologene: 5865
Man2a2
Name: mannosidase 2, alpha 2
Synonyms: 1700052O22Rik, 4931438M07Rik, alpha mannosidase IIx, MX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 140481
HGNC: HGNC:6825
Homologene: 55954
Kctd8
Name: potassium channel tetramerisation domain containing 8
Synonyms: A730087N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243043
Homologene: 18464
Scai
Name: suppressor of cancer cell invasion
Synonyms: A930041I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320271
Homologene: 15523
Fdps
Name: farnesyl diphosphate synthetase
Synonyms: Fdpsl1, 6030492I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 110196
HGNC: HGNC:3631
Homologene: 1519
Aldh1l2
Name: aldehyde dehydrogenase 1 family, member L2
Synonyms: D330038I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216188
Homologene: 51942
Fam75d3
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Prag1
Name: PEAK1 related kinase activating pseudokinase 1
Synonyms: NACK, D8Ertd82e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244418
Homologene: 18256
Or5an1c
Name: olfactory receptor family 5 subfamily AN member 1C
Synonyms: Olfr262, MOR214-1, GA_x6K02T2N4A9-18144-19082, MOR214-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258683
Ccdc66
Name: coiled-coil domain containing 66
Synonyms: E230015L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 320234
VEGA: 14
Homologene: 35309
Pank1
Name: pantothenate kinase 1
Synonyms: Pank1, 5430426F23Rik, Pank1a, Pank1b, 4632412I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75735
VEGA: 19
HGNC: HGNC:8598
Homologene: 56979
Actr3b
Name: ARP3 actin-related protein 3B
Synonyms: ARP11, Arp3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242894
Homologene: 4180
Phc2
Name: polyhomeotic 2
Synonyms: D4Ertd810e, Edr2, Mph2, D130050K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 54383
HGNC: HGNC:3183
Homologene: 75090
Zscan5b
Name: zinc finger and SCAN domain containing 5B
Synonyms: Zfg1, Zfp371
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170734
Homologene: 129555
Aldh5a1
Name: aldhehyde dehydrogenase family 5, subfamily A1
Synonyms: D630032B01Rik, SSADH, OTTMUSG00000000613, 6330403E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 214579
HGNC: HGNC:408
Homologene: 840
Dcun1d1
Name: DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
Synonyms: SCCRO, Tes3, pTes3, Rp42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 114893
Homologene: 10773
Sgsh
Name: N-sulfoglucosamine sulfohydrolase (sulfamidase)
Synonyms: 4632406A19Rik, sulphamidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27029
Homologene: 167
9230109A22Rik
Name: RIKEN cDNA 9230109A22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Lipo5
Name: lipase, member O5
Synonyms: Gm8975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 668095
Rhbdd2
Name: rhomboid domain containing 2
Synonyms: Rhbdl7, Usmg1, 0610011L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 215160
Homologene: 32481
Or8g28
Name: olfactory receptor family 8 subfamily G member 28
Synonyms: GA_x6K02T2PVTD-32955932-32954982, MOR171-20, Olfr945
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258499
VEGA: 9
HGNC: HGNC:8484
Homologene: 133643
C330011M18Rik
Name: RIKEN cDNA C330011M18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
1700001O22Rik
Name: RIKEN cDNA 1700001O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73598
Homologene: 18859
Chac2
Name: ChaC, cation transport regulator 2
Synonyms: 2510006C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68044
Homologene: 48696
Arhgef9
Name: CDC42 guanine nucleotide exchange factor (GEF) 9
Synonyms: 9630036L12Rik, collybistin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236915
Homologene: 9053
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 9,576,182 bp
  • A to G, chromosome 1 at 16,460,431 bp
  • G to T, chromosome 1 at 134,597,670 bp
  • A to T, chromosome 1 at 156,301,504 bp
  • A to G, chromosome 1 at 180,745,138 bp
  • C to T, chromosome 2 at 30,797,992 bp
  • T to C, chromosome 2 at 34,870,746 bp
  • A to T, chromosome 2 at 39,106,978 bp
  • T to A, chromosome 2 at 91,917,317 bp
  • G to A, chromosome 2 at 122,368,682 bp
  • A to T, chromosome 3 at 35,919,004 bp
  • T to C, chromosome 3 at 72,921,142 bp
  • A to G, chromosome 3 at 83,773,154 bp
  • T to A, chromosome 3 at 85,665,829 bp
  • A to T, chromosome 3 at 89,095,043 bp
  • C to T, chromosome 4 at 128,743,543 bp
  • A to G, chromosome 5 at 23,473,547 bp
  • T to C, chromosome 5 at 25,849,158 bp
  • T to A, chromosome 5 at 69,340,341 bp
  • T to A, chromosome 5 at 70,774,413 bp
  • G to A, chromosome 5 at 135,636,049 bp
  • G to A, chromosome 6 at 38,598,819 bp
  • A to G, chromosome 6 at 121,024,843 bp
  • C to A, chromosome 7 at 6,239,163 bp
  • G to A, chromosome 7 at 16,561,949 bp
  • G to T, chromosome 7 at 25,058,273 bp
  • G to T, chromosome 7 at 67,641,111 bp
  • A to G, chromosome 7 at 80,358,933 bp
  • T to C, chromosome 8 at 3,603,485 bp
  • T to A, chromosome 8 at 23,085,689 bp
  • A to G, chromosome 8 at 36,103,804 bp
  • T to C, chromosome 8 at 71,333,358 bp
  • A to T, chromosome 8 at 84,066,194 bp
  • A to G, chromosome 9 at 39,258,399 bp
  • T to C, chromosome 9 at 61,926,438 bp
  • A to G, chromosome 9 at 74,139,741 bp
  • T to G, chromosome 10 at 51,723,125 bp
  • A to T, chromosome 10 at 77,294,972 bp
  • C to A, chromosome 10 at 83,533,957 bp
  • T to A, chromosome 11 at 30,977,496 bp
  • A to T, chromosome 11 at 70,791,597 bp
  • G to T, chromosome 11 at 116,354,651 bp
  • A to G, chromosome 11 at 119,347,678 bp
  • T to C, chromosome 11 at 120,466,761 bp
  • T to C, chromosome 12 at 71,150,244 bp
  • A to G, chromosome 13 at 11,587,316 bp
  • T to C, chromosome 13 at 24,927,572 bp
  • A to T, chromosome 13 at 59,701,255 bp
  • T to C, chromosome 14 at 12,091,487 bp
  • G to A, chromosome 14 at 27,497,463 bp
  • C to A, chromosome 14 at 50,964,457 bp
  • A to T, chromosome 14 at 55,870,378 bp
  • A to T, chromosome 14 at 60,244,670 bp
  • A to G, chromosome 15 at 8,269,645 bp
  • T to C, chromosome 15 at 25,139,090 bp
  • T to A, chromosome 15 at 102,409,076 bp
  • T to A, chromosome 16 at 37,029,418 bp
  • C to A, chromosome 17 at 25,725,939 bp
  • A to G, chromosome 18 at 43,989,419 bp
  • T to C, chromosome 18 at 64,269,632 bp
  • A to G, chromosome 19 at 4,726,596 bp
  • A to T, chromosome 19 at 12,241,248 bp
  • A to T, chromosome 19 at 33,464,595 bp
  • A to G, chromosome 19 at 34,877,684 bp
  • T to A, chromosome 19 at 44,127,387 bp
  • A to T, chromosome 19 at 44,241,806 bp
  • T to A, chromosome 19 at 47,897,216 bp
  • A to G, chromosome 19 at 56,478,522 bp
  • T to A, chromosome X at 95,081,536 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1820 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039848-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.