Strain Name:
C57BL/6J-MtgxR1840Btlr/Mmmh
Stock Number:
039866-MU
Citation ID:
RRID:MMRRC_039866-MU
Other Names:
R1840 (G1), C57BL/6J-MtgxR1840Btlr
Major Collection:

Gene Information

Gene Symbol: Nrp2 [MGI:1100492] (Mus musculus (mouse))
Name: neuropilin 2
Synonyms: 1110048P06Rik, Npn-2, NP-2, Npn2, NP2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Tln2 [MGI:1917799] (Mus musculus (mouse))
Name: talin 2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Ptk2 [MGI:95481] (Mus musculus (mouse))
Name: PTK2 protein tyrosine kinase 2
Synonyms: FRNK, FAK, Fadk
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Hes5 [MGI:104876] (Mus musculus (mouse))
Name: hes family bHLH transcription factor 5
Synonyms: bHLHb38
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Eya1 [MGI:109344] (Mus musculus (mouse))
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Sez6 [MGI:104745] (Mus musculus (mouse))
Name: seizure related gene 6
Synonyms: D11Bhm177e, sez-6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Nrg4 [MGI:1933833] (Mus musculus (mouse))
Name: neuregulin 4
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 14,229,504 bp
  • T to C, chromosome 1 at 34,828,854 bp
  • T to C, chromosome 1 at 62,738,339 bp
  • T to A, chromosome 1 at 66,778,032 bp
  • A to G, chromosome 1 at 165,399,748 bp
  • A to T, chromosome 1 at 175,153,148 bp
  • A to G, chromosome 1 at 178,003,015 bp
  • T to A, chromosome 1 at 186,100,217 bp
  • G to A, chromosome 1 at 189,786,851 bp
  • T to C, chromosome 2 at 16,669,856 bp
  • T to C, chromosome 2 at 38,760,437 bp
  • T to A, chromosome 2 at 80,502,250 bp
  • G to A, chromosome 2 at 85,400,168 bp
  • T to C, chromosome 2 at 88,412,067 bp
  • T to C, chromosome 2 at 92,383,209 bp
  • T to C, chromosome 2 at 112,750,820 bp
  • T to C, chromosome 2 at 121,379,296 bp
  • A to G, chromosome 2 at 122,101,756 bp
  • T to A, chromosome 2 at 180,026,728 bp
  • G to A, chromosome 3 at 84,445,821 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to G, chromosome 3 at 108,150,481 bp
  • T to A, chromosome 3 at 135,357,468 bp
  • T to A, chromosome 4 at 9,601,340 bp
  • C to T, chromosome 4 at 56,903,947 bp
  • C to T, chromosome 4 at 59,219,517 bp
  • A to T, chromosome 4 at 118,365,657 bp
  • A to G, chromosome 4 at 123,241,390 bp
  • A to G, chromosome 4 at 129,118,600 bp
  • A to C, chromosome 4 at 137,572,184 bp
  • C to T, chromosome 4 at 149,188,132 bp
  • G to A, chromosome 4 at 149,906,631 bp
  • A to T, chromosome 4 at 149,982,050 bp
  • A to G, chromosome 4 at 154,961,254 bp
  • G to A, chromosome 4 at 156,167,415 bp
  • A to G, chromosome 5 at 24,500,231 bp
  • T to A, chromosome 5 at 108,104,139 bp
  • T to C, chromosome 5 at 113,819,844 bp
  • T to A, chromosome 5 at 135,249,571 bp
  • T to A, chromosome 6 at 12,330,974 bp
  • C to T, chromosome 6 at 42,363,588 bp
  • T to C, chromosome 6 at 57,071,537 bp
  • T to A, chromosome 6 at 57,658,106 bp
  • G to A, chromosome 6 at 71,398,455 bp
  • A to T, chromosome 6 at 104,774,480 bp
  • A to G, chromosome 7 at 14,254,829 bp
  • T to A, chromosome 7 at 18,610,438 bp
  • C to A, chromosome 7 at 64,759,195 bp
  • C to A, chromosome 7 at 90,452,884 bp
  • A to T, chromosome 7 at 99,767,596 bp
  • A to G, chromosome 7 at 104,004,117 bp
  • A to G, chromosome 8 at 33,742,348 bp
  • T to A, chromosome 8 at 61,969,553 bp
  • A to G, chromosome 8 at 95,365,420 bp
  • C to A, chromosome 8 at 104,126,616 bp
  • A to T, chromosome 9 at 24,725,676 bp
  • A to G, chromosome 9 at 46,286,408 bp
  • A to T, chromosome 9 at 53,456,530 bp
  • A to C, chromosome 9 at 55,282,606 bp
  • A to G, chromosome 9 at 56,620,558 bp
  • A to G, chromosome 9 at 58,140,530 bp
  • G to A, chromosome 9 at 67,342,043 bp
  • A to G, chromosome 9 at 75,249,735 bp
  • A to T, chromosome 9 at 86,815,465 bp
  • A to T, chromosome 9 at 92,258,074 bp
  • G to A, chromosome 9 at 95,670,117 bp
  • A to T, chromosome 9 at 95,670,203 bp
  • T to A, chromosome 10 at 7,710,939 bp
  • T to A, chromosome 10 at 50,690,161 bp
  • T to A, chromosome 10 at 58,478,766 bp
  • T to A, chromosome 10 at 62,763,510 bp
  • C to T, chromosome 10 at 78,812,946 bp
  • T to A, chromosome 10 at 84,604,599 bp
  • T to C, chromosome 10 at 89,422,251 bp
  • A to G, chromosome 10 at 99,022,929 bp
  • A to T, chromosome 10 at 126,013,389 bp
  • T to A, chromosome 11 at 49,216,388 bp
  • C to A, chromosome 11 at 55,395,866 bp
  • A to T, chromosome 11 at 65,834,198 bp
  • A to G, chromosome 11 at 67,186,487 bp
  • C to A, chromosome 11 at 77,953,717 bp
  • C to T, chromosome 11 at 86,233,085 bp
  • C to T, chromosome 11 at 90,685,056 bp
  • T to A, chromosome 11 at 100,745,341 bp
  • C to T, chromosome 11 at 101,295,566 bp
  • T to G, chromosome 11 at 120,013,732 bp
  • T to A, chromosome 12 at 16,928,989 bp
  • A to G, chromosome 12 at 33,371,033 bp
  • G to A, chromosome 12 at 84,158,350 bp
  • T to C, chromosome 12 at 85,803,221 bp
  • T to C, chromosome 12 at 88,329,411 bp
  • T to C, chromosome 12 at 101,147,862 bp
  • A to G, chromosome 13 at 8,979,464 bp
  • A to G, chromosome 13 at 34,178,056 bp
  • C to A, chromosome 13 at 34,960,041 bp
  • A to T, chromosome 13 at 49,901,765 bp
  • C to T, chromosome 13 at 70,606,218 bp
  • A to G, chromosome 13 at 73,974,652 bp
  • T to C, chromosome 13 at 92,511,525 bp
  • T to C, chromosome 13 at 95,981,844 bp
  • A to T, chromosome 13 at 103,834,947 bp
  • T to C, chromosome 14 at 52,313,725 bp
  • G to A, chromosome 14 at 70,534,776 bp
  • T to G, chromosome 14 at 121,994,262 bp
  • T to A, chromosome 15 at 5,031,879 bp
  • G to A, chromosome 15 at 37,980,917 bp
  • C to T, chromosome 15 at 47,607,164 bp
  • T to C, chromosome 15 at 73,210,884 bp
  • G to T, chromosome 15 at 76,120,642 bp
  • G to T, chromosome 15 at 81,427,353 bp
  • T to C, chromosome 15 at 86,130,192 bp
  • C to T, chromosome 16 at 11,161,026 bp
  • T to A, chromosome 16 at 21,653,486 bp
  • T to C, chromosome 16 at 32,826,172 bp
  • T to A, chromosome 16 at 35,863,449 bp
  • T to C, chromosome 16 at 46,099,092 bp
  • T to A, chromosome 17 at 27,111,458 bp
  • T to C, chromosome 17 at 32,543,006 bp
  • A to T, chromosome 17 at 37,850,748 bp
  • T to A, chromosome 17 at 55,610,406 bp
  • T to C, chromosome 17 at 87,999,641 bp
  • C to T, chromosome 18 at 11,734,473 bp
  • T to C, chromosome 18 at 20,682,809 bp
  • A to G, chromosome 19 at 3,886,460 bp
  • C to T, chromosome 19 at 4,801,795 bp
  • C to T, chromosome 19 at 5,609,238 bp
  • A to G, chromosome 19 at 18,866,267 bp
  • A to G, chromosome 19 at 56,842,312 bp
  • A to T, chromosome 19 at 60,534,250 bp
  • A to G, chromosome X at 111,420,932 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1840 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039866-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.