Strain Name:
C57BL/6J-MtgxR1843Btlr/Mmmh
Stock Number:
039868-MU
Citation ID:
RRID:MMRRC_039868-MU
Other Names:
R1843 (G1), C57BL/6J-MtgxR1843Btlr
Major Collection:

Gene Information

Gene Symbol: Chrnb4 [MGI:87892] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Evl [MGI:1194884] (Mus musculus (mouse))
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Atp1a1 [MGI:88105] (Mus musculus (mouse))
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Tfdp2 [MGI:107167] (Mus musculus (mouse))
Name: transcription factor Dp 2
Synonyms: DP3, 1110029I05Rik, A330080J22Rik, DP3, DP-3
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Actn4 [MGI:1890773] (Mus musculus (mouse))
Name: actinin alpha 4
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Nup210l [MGI:1924845] (Mus musculus (mouse))
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Armh3 [MGI:1918867] (Mus musculus (mouse))
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 56,636,632 bp
  • A to G, chromosome 1 at 106,343,505 bp
  • C to A, chromosome 1 at 106,778,982 bp
  • T to C, chromosome 1 at 133,914,757 bp
  • A to G, chromosome 1 at 153,754,674 bp
  • A to G, chromosome 1 at 163,950,675 bp
  • A to T, chromosome 1 at 167,598,752 bp
  • T to A, chromosome 2 at 50,188,762 bp
  • C to T, chromosome 2 at 122,292,258 bp
  • A to G, chromosome 2 at 129,102,966 bp
  • T to C, chromosome 2 at 164,463,297 bp
  • T to A, chromosome 3 at 54,279,994 bp
  • T to C, chromosome 3 at 67,435,610 bp
  • T to C, chromosome 3 at 90,172,086 bp
  • T to A, chromosome 3 at 93,446,780 bp
  • T to C, chromosome 3 at 96,682,219 bp
  • T to C, chromosome 3 at 101,582,017 bp
  • T to C, chromosome 3 at 105,679,082 bp
  • T to A, chromosome 3 at 106,732,811 bp
  • T to A, chromosome 4 at 48,422,035 bp
  • T to G, chromosome 4 at 55,010,010 bp
  • T to C, chromosome 4 at 58,785,384 bp
  • G to T, chromosome 4 at 70,534,785 bp
  • A to T, chromosome 4 at 99,961,478 bp
  • G to T, chromosome 4 at 116,877,699 bp
  • A to T, chromosome 4 at 121,622,030 bp
  • T to C, chromosome 4 at 137,545,567 bp
  • A to T, chromosome 4 at 139,363,788 bp
  • G to A, chromosome 4 at 139,375,512 bp
  • G to A, chromosome 4 at 139,784,491 bp
  • A to T, chromosome 4 at 152,385,806 bp
  • A to C, chromosome 5 at 49,961,492 bp
  • G to A, chromosome 5 at 110,330,835 bp
  • A to G, chromosome 5 at 121,297,180 bp
  • A to C, chromosome 6 at 50,370,143 bp
  • A to G, chromosome 6 at 54,522,841 bp
  • A to G, chromosome 6 at 91,265,775 bp
  • A to T, chromosome 6 at 114,862,795 bp
  • A to T, chromosome 6 at 122,464,009 bp
  • A to G, chromosome 6 at 128,645,369 bp
  • A to G, chromosome 6 at 130,229,994 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to T, chromosome 7 at 35,729,760 bp
  • C to T, chromosome 7 at 35,793,725 bp
  • T to A, chromosome 7 at 45,077,309 bp
  • G to A, chromosome 7 at 46,246,283 bp
  • T to C, chromosome 7 at 109,475,839 bp
  • A to C, chromosome 7 at 127,391,488 bp
  • A to G, chromosome 7 at 137,377,066 bp
  • T to A, chromosome 7 at 140,923,093 bp
  • A to T, chromosome 7 at 143,183,120 bp
  • T to A, chromosome 8 at 19,112,738 bp
  • T to A, chromosome 8 at 33,576,654 bp
  • T to C, chromosome 8 at 70,388,152 bp
  • C to T, chromosome 8 at 70,747,950 bp
  • T to A, chromosome 8 at 91,010,794 bp
  • C to T, chromosome 8 at 93,514,231 bp
  • C to T, chromosome 8 at 105,708,974 bp
  • A to T, chromosome 9 at 21,121,554 bp
  • A to G, chromosome 9 at 21,592,227 bp
  • A to T, chromosome 9 at 36,954,831 bp
  • A to G, chromosome 9 at 39,572,735 bp
  • A to T, chromosome 9 at 55,034,818 bp
  • G to T, chromosome 9 at 65,465,336 bp
  • T to C, chromosome 9 at 96,317,804 bp
  • T to A, chromosome 10 at 90,512,889 bp
  • T to C, chromosome 10 at 95,673,314 bp
  • T to A, chromosome 10 at 103,227,173 bp
  • T to C, chromosome 10 at 107,876,933 bp
  • C to T, chromosome 11 at 60,249,079 bp
  • G to C, chromosome 11 at 86,278,751 bp
  • CTAGTA to CTA, chromosome 11 at 99,333,526 bp
  • A to T, chromosome 11 at 115,869,586 bp
  • A to T, chromosome 11 at 116,684,332 bp
  • A to G, chromosome 11 at 121,285,537 bp
  • T to C, chromosome 12 at 8,007,602 bp
  • T to C, chromosome 12 at 81,980,935 bp
  • T to A, chromosome 12 at 103,895,023 bp
  • T to A, chromosome 12 at 108,652,996 bp
  • T to A, chromosome 12 at 111,322,821 bp
  • C to A, chromosome 13 at 21,674,672 bp
  • C to T, chromosome 13 at 33,671,381 bp
  • T to C, chromosome 13 at 49,655,170 bp
  • A to G, chromosome 13 at 81,544,533 bp
  • A to G, chromosome 14 at 30,518,983 bp
  • A to T, chromosome 14 at 31,038,957 bp
  • G to T, chromosome 14 at 32,546,820 bp
  • A to G, chromosome 14 at 34,249,171 bp
  • A to G, chromosome 14 at 34,267,803 bp
  • G to T, chromosome 14 at 69,719,799 bp
  • C to T, chromosome 14 at 70,164,779 bp
  • A to G, chromosome 14 at 122,490,765 bp
  • T to C, chromosome 15 at 79,737,563 bp
  • T to A, chromosome 15 at 100,365,249 bp
  • C to T, chromosome 15 at 102,113,133 bp
  • C to T, chromosome 16 at 20,229,564 bp
  • T to G, chromosome 16 at 20,656,582 bp
  • T to C, chromosome 16 at 57,276,780 bp
  • T to A, chromosome 16 at 58,824,077 bp
  • G to T, chromosome 17 at 3,669,878 bp
  • A to G, chromosome 17 at 12,704,270 bp
  • A to C, chromosome 17 at 18,244,470 bp
  • T to G, chromosome 17 at 18,597,921 bp
  • C to T, chromosome 17 at 24,641,719 bp
  • T to C, chromosome 17 at 48,448,790 bp
  • T to A, chromosome 17 at 55,956,679 bp
  • C to T, chromosome 17 at 86,475,546 bp
  • G to A, chromosome 18 at 37,975,583 bp
  • T to A, chromosome 18 at 38,192,225 bp
  • A to T, chromosome 18 at 43,999,891 bp
  • A to G, chromosome 18 at 61,518,050 bp
  • A to G, chromosome 18 at 82,584,122 bp
  • G to A, chromosome 18 at 89,073,532 bp
  • T to C, chromosome 19 at 4,271,595 bp
  • A to G, chromosome 19 at 13,479,931 bp
  • A to G, chromosome 19 at 39,877,528 bp
  • A to C, chromosome 19 at 45,975,252 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1843 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039868-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.