Strain Name:
C57BL/6J-MtgxR1852Btlr/Mmmh
Stock Number:
039876-MU
Citation ID:
RRID:MMRRC_039876-MU
Other Names:
R1852 (G1), C57BL/6J-MtgxR1852Btlr
Major Collection:

Gene Information

Gene Symbol: Pank4 [MGI:2387466] (Mus musculus (mouse))
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: Ankyrin-2, ankyrin B, Ankyrin-B, Gm4392, Ank-2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: PG-M, Cspg2, hdf, heart defect, DPEAAE, 5430420N07Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Cd36 [MGI:107899] (Mus musculus (mouse))
Name: CD36 molecule
Synonyms: fatty acid translocase, FAT, Scarb3
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Etv5 [MGI:1096867] (Mus musculus (mouse))
Name: ets variant 5
Synonyms: 8430401F14Rik, erm, 1110005E01Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Lars [MGI:1913808] (Mus musculus (mouse))
Name: leucyl-tRNA synthetase
Synonyms: 3110009L02Rik, 2310045K21Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Pcif1 [MGI:2443858] (Mus musculus (mouse))
Name: PDX1 C-terminal inhibiting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,159,279 bp
  • T to C, chromosome 1 at 74,549,361 bp
  • A to G, chromosome 1 at 75,146,675 bp
  • G to A, chromosome 1 at 85,085,131 bp
  • T to A, chromosome 1 at 90,807,534 bp
  • T to G, chromosome 1 at 139,446,866 bp
  • T to C, chromosome 2 at 52,228,542 bp
  • T to C, chromosome 2 at 87,710,973 bp
  • T to G, chromosome 2 at 88,006,521 bp
  • C to A, chromosome 2 at 112,091,847 bp
  • T to C, chromosome 2 at 120,071,644 bp
  • C to A, chromosome 2 at 131,081,500 bp
  • A to T, chromosome 2 at 156,178,955 bp
  • A to G, chromosome 2 at 157,174,903 bp
  • G to A, chromosome 2 at 164,888,466 bp
  • T to A, chromosome 2 at 172,416,553 bp
  • A to G, chromosome 2 at 174,423,978 bp
  • A to G, chromosome 3 at 63,327,983 bp
  • G to A, chromosome 3 at 63,328,046 bp
  • A to C, chromosome 3 at 64,259,394 bp
  • T to G, chromosome 3 at 85,618,877 bp
  • C to T, chromosome 3 at 106,148,801 bp
  • A to C, chromosome 3 at 126,997,851 bp
  • A to T, chromosome 3 at 141,857,402 bp
  • G to T, chromosome 4 at 47,299,278 bp
  • T to G, chromosome 4 at 99,746,362 bp
  • T to A, chromosome 4 at 126,097,963 bp
  • T to C, chromosome 4 at 134,507,669 bp
  • A to G, chromosome 4 at 137,521,730 bp
  • G to A, chromosome 4 at 141,622,268 bp
  • T to C, chromosome 4 at 143,812,826 bp
  • T to C, chromosome 4 at 154,976,359 bp
  • T to C, chromosome 5 at 17,811,236 bp
  • T to A, chromosome 5 at 21,290,545 bp
  • T to C, chromosome 5 at 29,446,878 bp
  • A to G, chromosome 5 at 35,093,391 bp
  • T to A, chromosome 5 at 88,504,465 bp
  • A to G, chromosome 5 at 101,915,376 bp
  • T to A, chromosome 5 at 114,970,711 bp
  • T to C, chromosome 5 at 123,991,505 bp
  • T to C, chromosome 5 at 134,382,580 bp
  • T to A, chromosome 5 at 137,580,156 bp
  • T to A, chromosome 5 at 138,979,172 bp
  • T to A, chromosome 5 at 142,711,950 bp
  • T to C, chromosome 6 at 43,275,185 bp
  • A to G, chromosome 6 at 47,520,830 bp
  • A to T, chromosome 6 at 103,699,159 bp
  • T to C, chromosome 6 at 108,386,706 bp
  • G to T, chromosome 6 at 123,139,125 bp
  • A to T, chromosome 6 at 133,853,673 bp
  • C to T, chromosome 7 at 4,144,039 bp
  • A to G, chromosome 7 at 12,512,702 bp
  • A to T, chromosome 7 at 30,658,501 bp
  • T to C, chromosome 7 at 35,787,751 bp
  • T to A, chromosome 7 at 40,993,613 bp
  • C to T, chromosome 7 at 102,928,441 bp
  • G to T, chromosome 7 at 122,114,314 bp
  • T to A, chromosome 7 at 140,176,561 bp
  • T to A, chromosome 8 at 19,239,975 bp
  • T to C, chromosome 8 at 70,300,983 bp
  • T to C, chromosome 8 at 71,483,487 bp
  • A to C, chromosome 8 at 91,095,563 bp
  • A to T, chromosome 8 at 94,937,800 bp
  • A to T, chromosome 8 at 106,874,079 bp
  • G to T, chromosome 8 at 119,626,208 bp
  • C to G, chromosome 9 at 46,221,089 bp
  • T to C, chromosome 10 at 7,120,539 bp
  • C to A, chromosome 10 at 9,812,298 bp
  • T to A, chromosome 10 at 19,743,019 bp
  • A to T, chromosome 10 at 77,021,877 bp
  • A to T, chromosome 10 at 81,642,941 bp
  • A to T, chromosome 10 at 92,686,309 bp
  • T to C, chromosome 10 at 129,174,328 bp
  • A to G, chromosome 11 at 5,287,455 bp
  • T to C, chromosome 11 at 8,958,993 bp
  • A to G, chromosome 11 at 16,995,194 bp
  • T to A, chromosome 11 at 51,653,865 bp
  • T to C, chromosome 11 at 54,361,076 bp
  • T to A, chromosome 11 at 54,642,811 bp
  • A to G, chromosome 11 at 61,310,042 bp
  • A to G, chromosome 11 at 78,268,473 bp
  • A to G, chromosome 11 at 82,808,507 bp
  • A to G, chromosome 11 at 106,171,221 bp
  • T to C, chromosome 11 at 110,069,386 bp
  • T to A, chromosome 11 at 118,121,916 bp
  • T to G, chromosome 11 at 120,723,494 bp
  • T to C, chromosome 11 at 120,921,673 bp
  • T to C, chromosome 12 at 29,851,661 bp
  • T to C, chromosome 12 at 101,149,161 bp
  • T to C, chromosome 13 at 9,137,303 bp
  • A to T, chromosome 13 at 19,261,091 bp
  • T to G, chromosome 13 at 35,896,914 bp
  • A to G, chromosome 13 at 41,011,232 bp
  • T to G, chromosome 13 at 89,705,392 bp
  • C to T, chromosome 13 at 97,143,096 bp
  • A to T, chromosome 13 at 106,812,257 bp
  • A to T, chromosome 13 at 112,872,927 bp
  • T to C, chromosome 14 at 55,704,941 bp
  • T to A, chromosome 14 at 60,843,240 bp
  • A to G, chromosome 14 at 72,556,843 bp
  • T to A, chromosome 14 at 73,164,680 bp
  • A to T, chromosome 15 at 43,867,163 bp
  • A to T, chromosome 15 at 89,306,568 bp
  • C to T, chromosome 15 at 92,305,140 bp
  • A to C, chromosome 16 at 22,411,931 bp
  • G to A, chromosome 16 at 48,287,884 bp
  • C to T, chromosome 16 at 78,941,258 bp
  • A to T, chromosome 16 at 97,448,203 bp
  • A to G, chromosome 17 at 18,063,174 bp
  • T to C, chromosome 17 at 29,702,255 bp
  • A to G, chromosome 17 at 57,222,823 bp
  • T to A, chromosome 17 at 68,840,617 bp
  • T to A, chromosome 17 at 88,765,176 bp
  • T to C, chromosome 18 at 42,212,608 bp
  • G to A, chromosome 18 at 61,849,395 bp
  • C to A, chromosome 18 at 77,016,023 bp
  • T to C, chromosome 18 at 86,395,884 bp
  • A to G, chromosome 19 at 6,009,103 bp
  • C to A, chromosome 19 at 11,896,885 bp
  • A to G, chromosome 19 at 12,776,847 bp
  • C to T, chromosome 19 at 13,619,603 bp
  • A to G, chromosome 19 at 17,199,139 bp
  • A to T, chromosome 19 at 18,962,083 bp
  • A to T, chromosome 19 at 25,127,128 bp
  • C to T, chromosome 19 at 39,617,367 bp
  • A to G, chromosome 19 at 46,372,180 bp
  • T to G, chromosome X at 102,864,645 bp
  • T to C, chromosome X at 104,305,399 bp
  • T to A, chromosome X at 116,478,061 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1852 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039876-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.