Strain Name:
C57BL/6J-MtgxR1857Btlr/Mmmh
Stock Number:
039881-MU
Citation ID:
RRID:MMRRC_039881-MU
Other Names:
R1857 (G1), C57BL/6J-MtgxR1857Btlr
Major Collection:

Gene Information

Gene Symbol: Vangl2 [MGI:2135272] (Mus musculus (mouse))
Name: VANGL planar cell polarity 2
Synonyms: C530001F03Rik, skam17Jus, strabismus, Lpp1, Lootl, loop-tail, ska17, Ltap
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Frem2 [MGI:2444465] (Mus musculus (mouse))
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, my, ne, b2b1562Clo, 6030440P17Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Eif3h [MGI:1915385] (Mus musculus (mouse))
Name: eukaryotic translation initiation factor 3, subunit H
Synonyms: EIF3-P40, EIF3-gamma, Eif3s3, 1110008A16Rik, 9430017H16Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Psmd7 [MGI:1351511] (Mus musculus (mouse))
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Synonyms: Mov34, Mov-34
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Pfdn1 [MGI:1914449] (Mus musculus (mouse))
Name: prefoldin 1
Synonyms: 2700086I23Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Mtor [MGI:1928394] (Mus musculus (mouse))
Name: mechanistic target of rapamycin kinase
Synonyms: 2610315D21Rik, RAPT1, RAFT1, mechanistic target of rapamycin (serine/threonine kinase), Frap1, FKBP-rapamycin-associated protein FRAP, flat
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Neurl4 [MGI:1921092] (Mus musculus (mouse))
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,247,905 bp
  • T to C, chromosome 1 at 143,770,750 bp
  • T to G, chromosome 1 at 161,970,877 bp
  • A to G, chromosome 1 at 163,039,145 bp
  • A to T, chromosome 1 at 172,009,897 bp
  • A to G, chromosome 1 at 181,450,073 bp
  • A to T, chromosome 2 at 24,292,805 bp
  • C to A, chromosome 2 at 70,476,869 bp
  • T to C, chromosome 2 at 87,418,648 bp
  • T to C, chromosome 2 at 106,783,644 bp
  • T to A, chromosome 2 at 127,770,376 bp
  • A to T, chromosome 3 at 28,903,390 bp
  • C to A, chromosome 3 at 45,379,937 bp
  • T to A, chromosome 3 at 53,654,873 bp
  • G to T, chromosome 4 at 11,053,474 bp
  • T to C, chromosome 4 at 96,659,486 bp
  • A to T, chromosome 4 at 100,441,503 bp
  • T to C, chromosome 4 at 101,835,573 bp
  • A to G, chromosome 4 at 111,656,318 bp
  • T to A, chromosome 4 at 113,483,862 bp
  • T to G, chromosome 4 at 132,113,681 bp
  • A to T, chromosome 4 at 138,175,876 bp
  • A to G, chromosome 4 at 138,569,102 bp
  • C to T, chromosome 4 at 144,208,521 bp
  • G to T, chromosome 4 at 148,480,879 bp
  • G to T, chromosome 5 at 5,582,518 bp
  • C to A, chromosome 5 at 74,212,301 bp
  • T to C, chromosome 5 at 86,904,382 bp
  • G to C, chromosome 5 at 104,814,857 bp
  • T to A, chromosome 5 at 145,566,850 bp
  • T to C, chromosome 5 at 151,095,438 bp
  • A to G, chromosome 6 at 89,844,615 bp
  • T to A, chromosome 6 at 143,960,815 bp
  • A to G, chromosome 7 at 28,750,012 bp
  • T to C, chromosome 7 at 67,679,073 bp
  • T to G, chromosome 7 at 105,121,544 bp
  • T to C, chromosome 7 at 120,361,369 bp
  • C to A, chromosome 7 at 139,653,408 bp
  • A to G, chromosome 7 at 141,207,508 bp
  • G to A, chromosome 8 at 72,200,460 bp
  • G to A, chromosome 8 at 85,328,594 bp
  • A to T, chromosome 8 at 107,584,893 bp
  • T to A, chromosome 8 at 111,040,157 bp
  • T to C, chromosome 8 at 117,040,669 bp
  • T to A, chromosome 9 at 14,357,109 bp
  • T to C, chromosome 9 at 18,312,045 bp
  • T to C, chromosome 9 at 49,278,676 bp
  • T to C, chromosome 9 at 58,032,708 bp
  • G to T, chromosome 9 at 59,314,965 bp
  • C to T, chromosome 9 at 87,041,279 bp
  • T to A, chromosome 9 at 95,865,097 bp
  • T to A, chromosome 9 at 101,212,893 bp
  • T to C, chromosome 9 at 104,021,294 bp
  • T to C, chromosome 10 at 34,153,229 bp
  • A to G, chromosome 10 at 58,235,780 bp
  • A to G, chromosome 10 at 60,323,297 bp
  • A to T, chromosome 10 at 75,928,357 bp
  • T to C, chromosome 10 at 76,166,735 bp
  • A to T, chromosome 10 at 93,531,554 bp
  • G to T, chromosome 11 at 69,905,535 bp
  • T to A, chromosome 11 at 75,495,423 bp
  • A to G, chromosome 11 at 101,320,820 bp
  • T to G, chromosome 12 at 13,413,519 bp
  • T to A, chromosome 12 at 110,662,625 bp
  • A to G, chromosome 13 at 21,347,176 bp
  • A to T, chromosome 13 at 27,659,180 bp
  • G to A, chromosome 13 at 55,506,045 bp
  • A to G, chromosome 14 at 32,376,674 bp
  • A to G, chromosome 15 at 34,678,633 bp
  • T to C, chromosome 15 at 51,799,278 bp
  • A to G, chromosome 15 at 78,452,552 bp
  • A to G, chromosome 15 at 99,709,414 bp
  • T to A, chromosome 15 at 102,446,777 bp
  • T to C, chromosome 16 at 37,082,166 bp
  • G to A, chromosome 16 at 55,860,298 bp
  • T to C, chromosome 17 at 20,961,689 bp
  • T to C, chromosome 17 at 24,823,570 bp
  • T to A, chromosome 17 at 26,742,963 bp
  • A to G, chromosome 18 at 6,441,107 bp
  • A to G, chromosome 18 at 23,971,467 bp
  • C to A, chromosome 18 at 36,451,100 bp
  • A to G, chromosome 18 at 43,766,835 bp
  • A to G, chromosome 18 at 62,515,418 bp
  • A to C, chromosome 19 at 60,782,197 bp
  • C to T, chromosome X at 37,157,126 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1857 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039881-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.