Strain Name:
C57BL/6J-MtgxR1865Btlr/Mmmh
Stock Number:
039888-MU
Citation ID:
RRID:MMRRC_039888-MU
Other Names:
R1865 (G1), C57BL/6J-MtgxR1865Btlr
Major Collection:

Gene Information

Gene Symbol: Rest [MGI:104897] (Mus musculus (mouse))
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ubn2 [MGI:2444236] (Mus musculus (mouse))
Name: ubinuclein 2
Synonyms: D130059P03Rik, 6030408G03Rik, 2900060J04Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Tagln3 [MGI:1926784] (Mus musculus (mouse))
Name: transgelin 3
Synonyms: Np25, 2700038H05Rik, 2900005O10Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Atn1 [MGI:104725] (Mus musculus (mouse))
Name: atrophin 1
Synonyms: atrophin-1, Drpla, Atr1
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Ctbp2 [MGI:1201686] (Mus musculus (mouse))
Name: C-terminal binding protein 2
Synonyms: D7Ertd45e, Gtrgeo6, Ribeye
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Itch [MGI:1202301] (Mus musculus (mouse))
Name: itchy, E3 ubiquitin protein ligase
Synonyms: AIP4, C230047C07Rik, 8030492O04Rik, 6720481N21Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Mcm7 [MGI:1298398] (Mus musculus (mouse))
Name: minichromosome maintenance complex component 7
Synonyms: mCDC47, Mcmd7
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,960,225 bp
  • A to G, chromosome 1 at 120,068,222 bp
  • A to T, chromosome 1 at 121,499,227 bp
  • A to T, chromosome 1 at 150,603,812 bp
  • A to G, chromosome 1 at 158,969,524 bp
  • T to C, chromosome 2 at 20,861,204 bp
  • T to C, chromosome 2 at 32,385,422 bp
  • T to C, chromosome 2 at 34,774,541 bp
  • T to A, chromosome 2 at 84,946,901 bp
  • T to G, chromosome 2 at 86,181,538 bp
  • T to C, chromosome 2 at 91,635,194 bp
  • A to T, chromosome 2 at 130,654,295 bp
  • G to A, chromosome 2 at 155,168,746 bp
  • C to A, chromosome 2 at 165,278,383 bp
  • G to A, chromosome 2 at 167,038,809 bp
  • T to C, chromosome 2 at 181,015,813 bp
  • A to G, chromosome 3 at 9,474,491 bp
  • A to T, chromosome 3 at 113,621,910 bp
  • A to T, chromosome 3 at 123,671,471 bp
  • A to T, chromosome 4 at 14,812,572 bp
  • A to T, chromosome 4 at 22,486,917 bp
  • A to G, chromosome 4 at 111,936,995 bp
  • A to G, chromosome 4 at 155,222,241 bp
  • GCTCT to GCTCTCT, chromosome 4 at 156,166,519 bp
  • G to T, chromosome 5 at 45,531,889 bp
  • A to G, chromosome 5 at 62,698,263 bp
  • T to A, chromosome 5 at 77,280,898 bp
  • T to A, chromosome 5 at 108,193,802 bp
  • A to T, chromosome 5 at 118,232,475 bp
  • A to T, chromosome 5 at 120,529,652 bp
  • A to T, chromosome 5 at 123,355,969 bp
  • C to A, chromosome 5 at 124,619,080 bp
  • A to C, chromosome 5 at 124,832,526 bp
  • A to T, chromosome 5 at 137,363,310 bp
  • T to A, chromosome 5 at 138,170,375 bp
  • A to T, chromosome 6 at 14,718,405 bp
  • G to A, chromosome 6 at 29,908,355 bp
  • A to G, chromosome 6 at 38,109,552 bp
  • T to A, chromosome 6 at 38,440,490 bp
  • T to A, chromosome 6 at 42,305,541 bp
  • G to A, chromosome 6 at 71,966,524 bp
  • A to T, chromosome 6 at 115,969,441 bp
  • T to C, chromosome 6 at 124,745,296 bp
  • T to A, chromosome 6 at 142,570,240 bp
  • A to T, chromosome 6 at 143,039,019 bp
  • T to C, chromosome 6 at 147,592,225 bp
  • T to C, chromosome 7 at 4,119,731 bp
  • T to C, chromosome 7 at 41,837,258 bp
  • A to G, chromosome 7 at 49,548,195 bp
  • G to A, chromosome 7 at 57,489,192 bp
  • T to C, chromosome 7 at 86,386,561 bp
  • A to G, chromosome 7 at 86,725,906 bp
  • A to G, chromosome 7 at 126,585,968 bp
  • C to T, chromosome 7 at 132,990,554 bp
  • A to T, chromosome 7 at 133,737,296 bp
  • G to A, chromosome 7 at 140,202,372 bp
  • A to G, chromosome 8 at 13,142,589 bp
  • A to G, chromosome 8 at 13,935,248 bp
  • A to T, chromosome 8 at 22,580,537 bp
  • T to C, chromosome 8 at 55,991,111 bp
  • A to G, chromosome 8 at 67,501,552 bp
  • A to G, chromosome 8 at 84,772,275 bp
  • A to G, chromosome 8 at 85,275,036 bp
  • G to T, chromosome 8 at 93,274,265 bp
  • G to A, chromosome 8 at 120,010,567 bp
  • T to A, chromosome 8 at 128,720,457 bp
  • T to C, chromosome 9 at 38,975,904 bp
  • A to C, chromosome 9 at 44,164,165 bp
  • G to A, chromosome 9 at 59,737,552 bp
  • A to G, chromosome 9 at 64,680,299 bp
  • A to C, chromosome 9 at 71,112,376 bp
  • A to T, chromosome 9 at 82,945,792 bp
  • A to T, chromosome 9 at 111,257,024 bp
  • A to G, chromosome 11 at 68,492,492 bp
  • G to A, chromosome 11 at 99,041,686 bp
  • T to G, chromosome 11 at 115,012,618 bp
  • G to A, chromosome 11 at 120,010,222 bp
  • T to A, chromosome 12 at 8,952,041 bp
  • G to T, chromosome 13 at 40,728,408 bp
  • T to A, chromosome 13 at 43,380,490 bp
  • G to T, chromosome 13 at 45,542,625 bp
  • T to C, chromosome 13 at 49,530,145 bp
  • A to G, chromosome 13 at 61,201,579 bp
  • G to A, chromosome 13 at 76,385,148 bp
  • T to C, chromosome 13 at 78,189,926 bp
  • A to C, chromosome 13 at 95,976,775 bp
  • T to A, chromosome 13 at 102,706,009 bp
  • A to T, chromosome 13 at 102,794,117 bp
  • T to A, chromosome 14 at 97,840,209 bp
  • T to C, chromosome 15 at 18,899,604 bp
  • T to C, chromosome 15 at 78,539,968 bp
  • A to T, chromosome 15 at 85,820,324 bp
  • A to G, chromosome 16 at 20,678,648 bp
  • T to A, chromosome 16 at 34,912,230 bp
  • A to G, chromosome 16 at 45,711,650 bp
  • A to G, chromosome 16 at 57,269,989 bp
  • A to T, chromosome 16 at 91,908,497 bp
  • A to G, chromosome 17 at 27,120,023 bp
  • C to T, chromosome 17 at 34,703,457 bp
  • T to C, chromosome 17 at 37,659,863 bp
  • A to G, chromosome 17 at 74,441,881 bp
  • T to A, chromosome 17 at 75,500,631 bp
  • A to G, chromosome 17 at 86,115,304 bp
  • A to G, chromosome 18 at 36,366,676 bp
  • T to C, chromosome 18 at 37,514,595 bp
  • A to G, chromosome 19 at 15,956,004 bp
  • C to A, chromosome 19 at 16,681,861 bp
  • G to T, chromosome 19 at 39,734,289 bp
  • G to A, chromosome X at 160,482,351 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1865 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039888-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.