Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR1885Btlr/Mmmh
Stock Number:
039906-MU
Citation ID:
RRID:MMRRC_039906-MU
Other Names:
R1885 (G1), C57BL/6J-MtgxR1885Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Apobec3 [MGI:1933111] (Mus musculus (mouse))
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Synonyms: Gm20117, Rfv3, Rfv-3, CEM15
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Dap3 [MGI:1929538] (Mus musculus (mouse))
Name: death associated protein 3
Synonyms: 4921514D13Rik, DAP-3
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Kl [MGI:1101771] (Mus musculus (mouse))
Name: klotho
Synonyms: alpha-kl
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Trdmt1 [MGI:1274787] (Mus musculus (mouse))
Name: tRNA aspartic acid methyltransferase 1
Synonyms: Rnmt2, Dnmt2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Adcy10 [MGI:2660854] (Mus musculus (mouse))
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, Sacy, 4930431D04Rik, sAC
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ncoa7 [MGI:2444847] (Mus musculus (mouse))
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ap2b1 [MGI:1919020] (Mus musculus (mouse))
Name: adaptor-related protein complex 2, beta 1 subunit
Synonyms: 1300012O03Rik
Chromosome: 11
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,935,480 bp
  • A to G, chromosome 1 at 165,570,808 bp
  • T to G, chromosome 2 at 13,523,414 bp
  • T to A, chromosome 2 at 20,743,984 bp
  • T to C, chromosome 2 at 87,532,824 bp
  • T to C, chromosome 2 at 104,230,455 bp
  • G to T, chromosome 2 at 111,664,754 bp
  • T to G, chromosome 2 at 147,049,361 bp
  • A to T, chromosome 3 at 8,541,904 bp
  • C to T, chromosome 3 at 27,279,296 bp
  • A to G, chromosome 3 at 88,930,974 bp
  • A to C, chromosome 3 at 105,904,836 bp
  • T to C, chromosome 3 at 130,629,462 bp
  • A to T, chromosome 3 at 157,543,156 bp
  • A to T, chromosome 4 at 63,170,417 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to C, chromosome 4 at 136,940,355 bp
  • G to A, chromosome 4 at 154,890,876 bp
  • T to C, chromosome 5 at 6,770,376 bp
  • A to T, chromosome 5 at 24,388,254 bp
  • A to G, chromosome 5 at 67,747,318 bp
  • A to G, chromosome 5 at 76,856,742 bp
  • C to T, chromosome 5 at 109,092,076 bp
  • A to G, chromosome 5 at 150,953,494 bp
  • T to C, chromosome 6 at 120,223,640 bp
  • G to A, chromosome 6 at 121,428,367 bp
  • G to T, chromosome 6 at 126,877,181 bp
  • A to T, chromosome 6 at 141,683,225 bp
  • T to C, chromosome 6 at 145,232,117 bp
  • A to T, chromosome 6 at 148,877,885 bp
  • T to C, chromosome 7 at 19,377,571 bp
  • T to C, chromosome 7 at 45,286,632 bp
  • G to A, chromosome 7 at 62,418,738 bp
  • T to C, chromosome 7 at 89,866,611 bp
  • G to A, chromosome 7 at 99,459,997 bp
  • A to T, chromosome 7 at 104,150,065 bp
  • A to G, chromosome 7 at 105,756,561 bp
  • A to G, chromosome 7 at 107,436,529 bp
  • T to C, chromosome 7 at 108,066,778 bp
  • A to T, chromosome 7 at 118,561,087 bp
  • A to G, chromosome 7 at 127,405,039 bp
  • T to G, chromosome 7 at 139,096,807 bp
  • T to A, chromosome 8 at 10,322,656 bp
  • A to T, chromosome 8 at 67,491,001 bp
  • T to C, chromosome 8 at 72,704,994 bp
  • A to G, chromosome 8 at 105,293,186 bp
  • A to G, chromosome 8 at 106,131,821 bp
  • T to C, chromosome 9 at 56,925,837 bp
  • A to G, chromosome 9 at 75,249,761 bp
  • T to A, chromosome 9 at 108,393,610 bp
  • C to A, chromosome 9 at 111,258,556 bp
  • T to C, chromosome 10 at 24,891,111 bp
  • T to C, chromosome 10 at 30,648,452 bp
  • A to G, chromosome 10 at 42,912,227 bp
  • C to T, chromosome 10 at 75,581,289 bp
  • G to A, chromosome 10 at 80,691,234 bp
  • T to C, chromosome 10 at 81,341,347 bp
  • A to G, chromosome 11 at 3,969,482 bp
  • T to C, chromosome 11 at 49,340,835 bp
  • A to G, chromosome 11 at 67,086,627 bp
  • C to T, chromosome 11 at 69,945,007 bp
  • C to A, chromosome 11 at 79,020,378 bp
  • T to C, chromosome 11 at 79,036,521 bp
  • T to A, chromosome 11 at 83,390,735 bp
  • C to A, chromosome 11 at 86,138,815 bp
  • T to A, chromosome 11 at 100,875,672 bp
  • T to C, chromosome 11 at 101,411,401 bp
  • T to C, chromosome 11 at 105,131,620 bp
  • T to A, chromosome 12 at 4,134,951 bp
  • C to A, chromosome 12 at 84,372,464 bp
  • G to T, chromosome 13 at 45,567,804 bp
  • T to G, chromosome 13 at 59,702,007 bp
  • A to T, chromosome 13 at 76,113,047 bp
  • A to G, chromosome 13 at 76,130,235 bp
  • C to T, chromosome 14 at 8,248,102 bp
  • A to C, chromosome 14 at 13,828,607 bp
  • T to A, chromosome 14 at 16,383,226 bp
  • T to C, chromosome 14 at 64,028,390 bp
  • G to T, chromosome 14 at 120,919,908 bp
  • A to G, chromosome 15 at 31,502,806 bp
  • G to T, chromosome 15 at 75,926,989 bp
  • A to T, chromosome 15 at 79,897,705 bp
  • A to G, chromosome 15 at 80,211,619 bp
  • A to G, chromosome 15 at 80,358,944 bp
  • A to G, chromosome 16 at 25,412,891 bp
  • A to C, chromosome 16 at 45,096,720 bp
  • T to C, chromosome 16 at 73,916,145 bp
  • ATCTTCT to ATCT, chromosome 17 at 23,359,988 bp
  • T to C, chromosome 17 at 46,262,421 bp
  • T to A, chromosome 17 at 64,138,914 bp
  • A to T, chromosome 17 at 78,932,116 bp
  • A to G, chromosome 17 at 86,805,295 bp
  • G to A, chromosome 18 at 24,601,482 bp
  • G to A, chromosome 19 at 11,752,324 bp
  • T to C, chromosome 19 at 56,860,252 bp
  • GTCATCATCATCATC to GTCATCATCATCATCATC, chromosome X at 6,082,591 bp
  • G to A, chromosome X at 73,795,446 bp
  • G to T, chromosome Y at 940,781 bp
Genotype Determination
ES Cell Line
N/A

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice

    Strain Origin

    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1885 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

    Appearance

    Coat Color
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

    Reproductive Statistics

    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039906-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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