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Strain Name:
C57BL/6J-MtgxR1961Btlr/Mmmh
Stock Number:
039975-MU
Citation ID:
RRID:MMRRC_039975-MU
Other Names:
R1961 (G1), C57BL/6J-MtgxR1961Btlr
Major Collection:

Strain Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Btrc
Name: beta-transducin repeat containing protein
Synonyms: SCF b-TRCP, beta-TrCP, Slimb, Beta-Trcp1, Fbw1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330
Tnpo1
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238799
HGNC: HGNC:6401
Homologene: 5358
Nr2f2
Name: nuclear receptor subfamily 2, group F, member 2
Synonyms: COUP-TFII, ARP-1, Tcfcoup2, Aporp1, COUP-TF2, 9430015G03Rik, EAR3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11819
HGNC: HGNC:7976
Homologene: 7628
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Glur-4, Glur4, spkw1, Gluralpha4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Sptlc1
Name: serine palmitoyltransferase, long chain base subunit 1
Synonyms: Lcb1, Spt1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 268656
VEGA: 13
Homologene: 4681
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 74,281,513 bp
  • G to A, chromosome 1 at 83,022,798 bp
  • CAGAGAGA to CAGAGAGAGA, chromosome 1 at 85,577,224 bp
  • A to G, chromosome 1 at 177,096,995 bp
  • T to C, chromosome 2 at 29,197,098 bp
  • A to T, chromosome 2 at 51,948,620 bp
  • A to T, chromosome 2 at 65,330,339 bp
  • A to T, chromosome 2 at 76,721,760 bp
  • T to A, chromosome 2 at 76,798,212 bp
  • T to A, chromosome 2 at 80,424,601 bp
  • A to G, chromosome 2 at 137,067,681 bp
  • G to A, chromosome 3 at 27,456,451 bp
  • C to T, chromosome 3 at 88,438,176 bp
  • A to G, chromosome 3 at 135,242,493 bp
  • A to T, chromosome 3 at 146,046,494 bp
  • A to G, chromosome 3 at 146,915,795 bp
  • T to C, chromosome 3 at 152,380,628 bp
  • C to A, chromosome 4 at 6,688,886 bp
  • T to G, chromosome 4 at 43,369,688 bp
  • A to G, chromosome 4 at 102,597,460 bp
  • A to G, chromosome 4 at 112,858,162 bp
  • T to C, chromosome 4 at 129,640,262 bp
  • G to A, chromosome 4 at 132,377,248 bp
  • CTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC to CTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC, chromosome 4 at 155,614,472 bp
  • G to A, chromosome 5 at 71,700,336 bp
  • T to A, chromosome 5 at 108,798,095 bp
  • C to T, chromosome 5 at 112,424,615 bp
  • T to C, chromosome 6 at 61,313,646 bp
  • T to C, chromosome 6 at 63,908,893 bp
  • C to A, chromosome 6 at 85,348,991 bp
  • T to C, chromosome 6 at 86,096,756 bp
  • A to G, chromosome 6 at 96,165,269 bp
  • A to T, chromosome 6 at 118,630,322 bp
  • T to C, chromosome 6 at 119,969,247 bp
  • A to G, chromosome 6 at 120,982,607 bp
  • A to C, chromosome 6 at 129,350,481 bp
  • A to C, chromosome 6 at 131,168,162 bp
  • A to G, chromosome 6 at 148,184,186 bp
  • T to C, chromosome 7 at 5,327,738 bp
  • C to A, chromosome 7 at 5,481,071 bp
  • G to T, chromosome 7 at 33,527,378 bp
  • G to T, chromosome 7 at 70,358,155 bp
  • T to C, chromosome 7 at 103,219,997 bp
  • T to C, chromosome 7 at 119,708,740 bp
  • T to A, chromosome 7 at 121,118,569 bp
  • A to G, chromosome 8 at 31,124,846 bp
  • T to A, chromosome 8 at 95,540,831 bp
  • A to G, chromosome 8 at 105,381,464 bp
  • T to G, chromosome 8 at 109,530,144 bp
  • C to A, chromosome 8 at 117,131,038 bp
  • A to T, chromosome 9 at 4,519,546 bp
  • A to G, chromosome 9 at 119,452,266 bp
  • A to C, chromosome 10 at 21,388,930 bp
  • T to A, chromosome 10 at 33,450,967 bp
  • A to T, chromosome 10 at 42,823,886 bp
  • A to G, chromosome 10 at 51,590,334 bp
  • A to T, chromosome 10 at 67,039,499 bp
  • C to T, chromosome 11 at 3,490,983 bp
  • T to G, chromosome 11 at 51,027,475 bp
  • T to G, chromosome 11 at 53,372,999 bp
  • C to T, chromosome 11 at 75,416,419 bp
  • T to C, chromosome 11 at 95,491,711 bp
  • A to G, chromosome 11 at 121,644,525 bp
  • A to G, chromosome 12 at 69,953,500 bp
  • A to T, chromosome 12 at 81,559,508 bp
  • G to A, chromosome 13 at 46,864,838 bp
  • A to C, chromosome 13 at 53,358,880 bp
  • T to G, chromosome 13 at 58,293,123 bp
  • T to C, chromosome 13 at 67,763,915 bp
  • C to A, chromosome 13 at 77,902,720 bp
  • T to C, chromosome 13 at 98,852,932 bp
  • T to C, chromosome 14 at 21,041,015 bp
  • A to G, chromosome 14 at 34,398,475 bp
  • G to T, chromosome 14 at 66,156,442 bp
  • T to A, chromosome 14 at 108,912,190 bp
  • C to A, chromosome 14 at 118,611,456 bp
  • C to A, chromosome 14 at 118,611,459 bp
  • T to C, chromosome 15 at 38,295,996 bp
  • G to A, chromosome 15 at 76,297,464 bp
  • T to A, chromosome 15 at 76,662,825 bp
  • A to G, chromosome 15 at 85,511,044 bp
  • T to C, chromosome 15 at 89,557,964 bp
  • G to A, chromosome 15 at 90,970,848 bp
  • T to A, chromosome 16 at 13,677,728 bp
  • T to C, chromosome 16 at 14,396,393 bp
  • T to C, chromosome 16 at 20,602,191 bp
  • T to C, chromosome 16 at 23,452,932 bp
  • T to A, chromosome 16 at 58,718,007 bp
  • A to C, chromosome 16 at 96,378,351 bp
  • T to C, chromosome 17 at 29,704,431 bp
  • T to C, chromosome 17 at 33,933,615 bp
  • A to C, chromosome 17 at 46,480,224 bp
  • A to T, chromosome 17 at 55,662,531 bp
  • T to C, chromosome 17 at 55,791,497 bp
  • T to A, chromosome 17 at 74,780,856 bp
  • T to A, chromosome 18 at 57,212,354 bp
  • G to A, chromosome 18 at 61,061,505 bp
  • C to A, chromosome 19 at 8,169,193 bp
  • G to A, chromosome 19 at 12,462,911 bp
  • T to A, chromosome 19 at 37,687,377 bp
  • T to G, chromosome 19 at 45,527,343 bp
  • A to T, chromosome 19 at 61,297,007 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1961 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039975-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.