Strain Name:
C57BL/6J-MtgxR1995Btlr/Mmmh
Stock Number:
040005-MU
Citation ID:
RRID:MMRRC_040005-MU
Other Names:
R1995 (G1), C57BL/6J-MtgxR1995Btlr
Major Collection:

Gene Information

Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Jarid2
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Tbcel
Name: tubulin folding cofactor E-like
Synonyms: E130107N23Rik, Lrrc35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 272589
Homologene: 16120
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Pogz
Name: pogo transposable element with ZNF domain
Synonyms: 9530006B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229584
Homologene: 9022
Pcnt
Name: pericentrin (kendrin)
Synonyms: Pcnt2, m275Asp, m239Asp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18541
VEGA: 10
Btbd9
Name: BTB (POZ) domain containing 9
Synonyms: 1700023F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224671
VEGA: 17
Homologene: 14995
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Prrc2a
Name: proline-rich coiled-coil 2A
Synonyms: G2, Bat-2, D17H6S51E, Wbp12, 3110039B05Rik, Bat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 53761
Homologene: 10567
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: 5230400C17Rik, Pip5k3, PipkIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18711
Homologene: 32115
Rock1
Name: Rho-associated coiled-coil containing protein kinase 1
Synonyms: Rock-I, 1110055K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 19877
VEGA: 18
Homologene: 55899
Kdm8
Name: lysine (K)-specific demethylase 8
Synonyms: 3110005O21Rik, Jmjd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77035
Homologene: 49791
Hipk2
Name: homeodomain interacting protein kinase 2
Synonyms: Stank, 1110014O20Rik, B230339E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15258
Homologene: 68766
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 404710
Homologene: 26400
Cep120
Name: centrosomal protein 120
Synonyms: Ccdc100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225523
VEGA: 18
Homologene: 27415
Dmp1
Name: dentin matrix protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13406
HGNC: HGNC:2932
Homologene: 68396
Pms1
Name: PMS1 homolog 1, mismatch repair system component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227099
HGNC: HGNC:9121
Homologene: 449
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Sdccag8
Name: serologically defined colon cancer antigen 8
Synonyms: CCCAP, 2700048G21Rik, 5730470G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76816
Homologene: 4839
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319675
Homologene: 27936
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22787
Homologene: 48194
Agtpbp1
Name: ATP/GTP binding protein 1
Synonyms: Nna1, 2900054O13Rik, 4930445M19Rik, 1700020N17Rik, 5730402G09Rik, 2310001G17Rik, Ccp1, atms
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67269
Homologene: 9067
Cnbd1
Name: cyclic nucleotide binding domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435772
Homologene: 27843
Cenpl
Name: centromere protein L
Synonyms: 2610300B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70454
Homologene: 19618
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Ttll7
Name: tubulin tyrosine ligase-like family, member 7
Synonyms: 1110049N09Rik, 4921517B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70892
Homologene: 41578
Mfsd12
Name: major facilitator superfamily domain containing 12
Synonyms: Wdt1, F630110N24Rik, gr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73822
Homologene: 45488
Eral1
Name: Era (G-protein)-like 1 (E. coli)
Synonyms: MERA-S, MERA-W, 9130407C09Rik, 2610524P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57837
HGNC: HGNC:3424
Homologene: 4167
Slc9c1
Name: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Synonyms: LOC208169, spermNHE, Slc9a10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208169
Homologene: 19505
Olfr965
Name: olfactory receptor 965
Synonyms: GA_x6K02T2PVTD-33416730-33417668, MOR171-28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258165
VEGA: 9
Pik3r4
Name: phosphoinositide-3-kinase regulatory subunit 4
Synonyms: Vps15, p150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75669
HGNC: HGNC:8982
Homologene: 24678
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: unc-33-like phosphoprotein 4, Ulip4, CRMP-3, Drp-4, DPY4, Crmp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: beta 1 sGC, Gucy1b3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Gm4934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
H2-M9
Name: histocompatibility 2, M region locus 9
Synonyms: M9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14997
Homologene: 110792
Cyp2j9
Name: cytochrome P450, family 2, subfamily j, polypeptide 9
Synonyms: 8430417E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74519
Homologene: 137388
Ltbp2
Name: latent transforming growth factor beta binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16997
HGNC: HGNC:6715
Homologene: 369
Olfr205
Name: olfactory receptor 205
Synonyms: GA_x54KRFPKG5P-55543875-55542958, MOR182-11P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 257881
Homologene: 79469
Speer2
Name: spermatogenesis associated glutamate (E)-rich protein 2
Synonyms: SPEER-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224318
Homologene: 69402
Flad1
Name: flavin adenine dinucleotide synthetase 1
Synonyms: Pp591, A930017E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 319945
Homologene: 115598
Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Nuggc
Name: nuclear GTPase, germinal center associated
Synonyms: LOC239151, SLIP-GC, Gm600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100503545
Homologene: 72641
Tmcc3
Name: transmembrane and coiled coil domains 3
Synonyms: C630016B22Rik, LOC380656, A230066D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 319880
Homologene: 10792
AY358078
Name: cDNA sequence AY358078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 278676
Homologene: 115686
Chil3
Name: chitinase-like 3
Synonyms: Ym1, Chi3l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12655
Homologene: 74931
Dcbld2
Name: discoidin, CUB and LCCL domain containing 2
Synonyms: 1700055P21Rik, Esdn, CLCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73379
Homologene: 12499
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2610019B15Rik, 2810470M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67242
Homologene: 11711
Itprid1
Name: ITPR interacting domain containing 1
Synonyms: D530004J12Rik, Ccdc129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232016
Homologene: 52344
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Kcnb2
Name: potassium voltage gated channel, Shab-related subfamily, member 2
Synonyms: Kv2.2, 9630047L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98741
HGNC: HGNC:6232
Homologene: 31263
Olfr1161
Name: olfactory receptor 1161
Synonyms: GA_x6K02T2Q125-49516664-49517629, MOR174-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258845
Homologene: 138318
Pkn3
Name: protein kinase N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 263803
Homologene: 50980
Sphkap
Name: SPHK1 interactor, AKAP domain containing
Synonyms: A930009L15Rik, 4930544G21Rik, SKIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77629
Homologene: 18172
Psg18
Name: pregnancy specific glycoprotein 18
Synonyms: mmCGM6, Cea-3, Cea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26438
Homologene: 110989
Ctnna3
Name: catenin (cadherin associated protein), alpha 3
Synonyms: Vr22, Catna3, 4930429L08Rik, alphaT-catenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216033
HGNC: HGNC:2511
Homologene: 56583
Serpine2
Name: serine (or cysteine) peptidase inhibitor, clade E, member 2
Synonyms: nexin, PN-1, PI7, protease nexin 1, Spi4, B230326M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20720
HGNC: HGNC:8951
Homologene: 21247
Vmn1r177
Name: vomeronasal 1 receptor 177
Synonyms: V1rd12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384572
Homologene: 122945
Slco2a1
Name: solute carrier organic anion transporter family, member 2a1
Synonyms: Pgt, 2310021C19Rik, mPgt, Slc21a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24059
Homologene: 38077
Cdc25b
Name: cell division cycle 25B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12531
HGNC: HGNC:1726
Homologene: 41451
Gpbar1
Name: G protein-coupled bile acid receptor 1
Synonyms: GPR131, M-BAR, BG37, TGR5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227289
Homologene: 18125
Tmem253
Name: transmembrane protein 253
Synonyms: G630016D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 619301
VEGA: 14
Homologene: 83921
Fbxo40
Name: F-box protein 40
Synonyms: 9830003A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207215
Homologene: 9459
Catsperb
Name: cation channel sperm associated auxiliary subunit beta
Synonyms: 4932415G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 271036
VEGA: 12
Homologene: 81904
Scd4
Name: stearoyl-coenzyme A desaturase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329065
Homologene: 77322
Olfr1122
Name: olfactory receptor 1122
Synonyms: GA_x6K02T2Q125-48880078-48881058, MOR264-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259033
Homologene: 73977
Nkain3
Name: Na+/K+ transporting ATPase interacting 3
Synonyms: E130310K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269513
Homologene: 18265
Nkain2
Name: Na+/K+ transporting ATPase interacting 2
Synonyms: 6330571D19Rik, Tcba1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432450
Homologene: 65073
Cnih2
Name: cornichon family AMPA receptor auxiliary protein 2
Synonyms: Cnil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 12794
VEGA: 19
Homologene: 7671
Tmem240
Name: transmembrane protein 240
Synonyms: Gm5151
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381582
Homologene: 19885
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 15,709,766 bp
  • A to G, chromosome 1 at 53,195,015 bp
  • T to A, chromosome 1 at 65,246,708 bp
  • G to A, chromosome 1 at 74,279,444 bp
  • A to G, chromosome 1 at 79,821,442 bp
  • G to A, chromosome 1 at 83,277,515 bp
  • T to C, chromosome 1 at 161,078,424 bp
  • A to C, chromosome 1 at 176,874,560 bp
  • G to C, chromosome 2 at 30,089,977 bp
  • C to T, chromosome 2 at 52,298,732 bp
  • T to G, chromosome 2 at 87,387,831 bp
  • T to A, chromosome 2 at 88,025,672 bp
  • T to A, chromosome 2 at 125,350,373 bp
  • T to A, chromosome 2 at 131,188,092 bp
  • G to A, chromosome 3 at 80,802,357 bp
  • A to G, chromosome 3 at 82,034,853 bp
  • G to T, chromosome 3 at 87,095,786 bp
  • A to G, chromosome 3 at 88,101,884 bp
  • T to C, chromosome 3 at 89,411,146 bp
  • C to T, chromosome 3 at 94,877,944 bp
  • T to C, chromosome 3 at 106,148,566 bp
  • G to A, chromosome 3 at 146,961,755 bp
  • G to A, chromosome 4 at 19,055,112 bp
  • A to G, chromosome 4 at 20,469,432 bp
  • G to A, chromosome 4 at 96,583,578 bp
  • T to A, chromosome 4 at 155,739,847 bp
  • T to G, chromosome 5 at 104,209,913 bp
  • C to A, chromosome 6 at 38,715,974 bp
  • T to C, chromosome 6 at 55,968,709 bp
  • A to G, chromosome 7 at 18,353,678 bp
  • T to A, chromosome 7 at 23,865,687 bp
  • C to T, chromosome 7 at 84,602,181 bp
  • T to C, chromosome 7 at 116,354,006 bp
  • T to A, chromosome 7 at 120,135,165 bp
  • G to A, chromosome 7 at 125,452,339 bp
  • A to G, chromosome 7 at 139,096,770 bp
  • C to T, chromosome 9 at 15,340,883 bp
  • T to C, chromosome 9 at 39,719,413 bp
  • C to A, chromosome 9 at 42,451,661 bp
  • T to G, chromosome 9 at 103,076,909 bp
  • T to A, chromosome 9 at 105,669,165 bp
  • A to G, chromosome 10 at 32,402,351 bp
  • T to A, chromosome 10 at 63,820,364 bp
  • G to A, chromosome 10 at 76,392,799 bp
  • T to C, chromosome 10 at 76,721,956 bp
  • C to A, chromosome 10 at 81,357,681 bp
  • T to C, chromosome 10 at 94,578,606 bp
  • C to T, chromosome 11 at 78,074,489 bp
  • A to G, chromosome 11 at 107,068,260 bp
  • T to G, chromosome 12 at 84,808,446 bp
  • T to A, chromosome 12 at 101,602,767 bp
  • T to C, chromosome 13 at 44,874,441 bp
  • T to G, chromosome 13 at 59,531,058 bp
  • G to C, chromosome 13 at 59,716,380 bp
  • T to A, chromosome 14 at 51,826,062 bp
  • A to C, chromosome 14 at 52,018,534 bp
  • A to G, chromosome 14 at 65,611,174 bp
  • T to C, chromosome 16 at 36,969,869 bp
  • A to C, chromosome 16 at 45,554,255 bp
  • A to C, chromosome 16 at 58,456,332 bp
  • A to G, chromosome 16 at 59,329,291 bp
  • A to G, chromosome 16 at 69,858,077 bp
  • A to G, chromosome 17 at 30,274,930 bp
  • A to T, chromosome 17 at 35,157,429 bp
  • A to T, chromosome 17 at 36,641,786 bp
  • A to C, chromosome 17 at 80,227,985 bp
  • G to A, chromosome 18 at 10,101,026 bp
  • G to A, chromosome 18 at 53,740,136 bp
  • G to T, chromosome 18 at 63,078,781 bp
  • T to A, chromosome 19 at 5,093,510 bp
  • T to A, chromosome 19 at 44,334,178 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1995 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040005-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.