Strain Name:
C57BL/6J-MtgxR2009Btlr/Mmmh
Stock Number:
040018-MU
Citation ID:
RRID:MMRRC_040018-MU
Other Names:
R2009 (G1), C57BL/6J-MtgxR2009Btlr
Major Collection:

Gene Information

Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56632
Homologene: 32456
Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, separase, SSE, ESP1, PRCE, Cerp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Etv4
Name: ets variant 4
Synonyms: Pea-3, Pea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18612
HGNC: HGNC:3493
Homologene: 1504
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: wolframin, Wolfram syndrome 1 homolog (human)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22393
Homologene: 4380
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Eif4g2
Name: eukaryotic translation initiation factor 4, gamma 2
Synonyms: Nat1, DAP-5, Natm1, E130105L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13690
HGNC: HGNC:3297
Homologene: 37477
Mib1
Name: mindbomb E3 ubiquitin protein ligase 1
Synonyms: E430019M12Rik, Mib, skeletrophin, mind bomb-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225164
Homologene: 10810
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Zc3h3
Name: zinc finger CCCH type containing 3
Synonyms: Smicl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223642
VEGA: 15
Homologene: 9029
Sgk1
Name: serum/glucocorticoid regulated kinase 1
Synonyms: Sgk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20393
Homologene: 48364
Trim39
Name: tripartite motif-containing 39
Synonyms: RING-B box-coiled-coil-B30.2, RBCC-B30.2, tfp, 1100001D15Rik, Rnf23, E130103K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 79263
Homologene: 10940
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207212
Homologene: 129601
Dmp1
Name: dentin matrix protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13406
HGNC: HGNC:2932
Homologene: 68396
Limd1
Name: LIM domains containing 1
Synonyms: D9Ertd192e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 29806
VEGA: 9
HGNC: HGNC:6612
Homologene: 8478
Wdr4
Name: WD repeat domain 4
Synonyms: D530049K22Rik, Wh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57773
Homologene: 32422
Lap3
Name: leucine aminopeptidase 3
Synonyms: Pep-S, Pep-7, LAP, peptidase S, Pep7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66988
Homologene: 41072
Map4k3
Name: mitogen-activated protein kinase kinase kinase kinase 3
Synonyms: 9530052P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 225028
VEGA: 17
HGNC: HGNC:6865
Homologene: 2683
Szt2
Name: SZT2 subunit of KICSTOR complex
Synonyms: seaizure threshold 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230676
Homologene: 49413
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 544678
Homologene: 129584
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Zfp750
Name: zinc finger protein 750
Synonyms: A030007D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319530
Homologene: 36423
Trappc14
Name: trafficking protein particle complex 14
Synonyms: Map11, BC037034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231807
Homologene: 10106
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Trpm5
Name: transient receptor potential cation channel, subfamily M, member 5
Synonyms: Mtr1, Ltrpc5, 9430099A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56843
Homologene: 22818
Trim60
Name: tripartite motif-containing 60
Synonyms: 2czf45, Rnf33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234329
Homologene: 51876
Gne
Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Synonyms: 2310066H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50798
Homologene: 3996
Adamts15
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235130
VEGA: 9
Homologene: 8610
Nrg3
Name: neuregulin 3
Synonyms: ska
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18183
HGNC: HGNC:7999
Homologene: 32051
Gabbr2
Name: gamma-aminobutyric acid (GABA) B receptor, 2
Synonyms: Gababr2, LOC242425, Gpr51, GB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242425
HGNC: HGNC:4507
Homologene: 55902
Adgrf1
Name: adhesion G protein-coupled receptor F1
Synonyms: 5031409J19Rik, Gpr110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77596
Homologene: 124643
Pfpl
Name: pore forming protein-like
Synonyms: Epcs5, Epcs50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56093
VEGA: 19
Homologene: 130704
Vmn2r107
Name: vomeronasal 2, receptor 107
Synonyms: V2r6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22312
Homologene: 129750
Asz1
Name: ankyrin repeat, SAM and basic leucine zipper domain containing 1
Synonyms: ORF3, 4933400N19Rik, Gasz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74068
HGNC: HGNC:1350
Homologene: 11374
Olfr248
Name: olfactory receptor 248
Synonyms: GA_x6K02T2P20D-20771141-20770212, GA_x6K02T2MFC0-1145-1312, MOR267-7, Olfr415
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258709
C1s2
Name: complement component 1, s subcomponent 2
Synonyms: Gm5077
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 317677
HGNC: HGNC:1247
Homologene: 1314
Adgrg7
Name: adhesion G protein-coupled receptor G7
Synonyms: 9130020O16Rik, Gpr128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239853
Homologene: 13115
Pkd2l1
Name: polycystic kidney disease 2-like 1
Synonyms: polycystin-L, Pkdl, PCL, TRPP3, PKD2L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329064
HGNC: HGNC:9011
Homologene: 22946
Nkapl
Name: NFKB activating protein-like
Synonyms: 4921504I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66707
Homologene: 57034
Olfr549
Name: olfactory receptor 549
Synonyms: GA_x6K02T2PBJ9-5274337-5275287, MOR31-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259105
Homologene: 86245
Wnt2
Name: wingless-type MMTV integration site family, member 2
Synonyms: Wnt-2, Irp, Int1l1, m-irp, Mirp, 2610510E18Rik, Wnt2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22413
Homologene: 20719
Rnase2b
Name: ribonuclease, RNase A family, 2B (liver, eosinophil-derived neurotoxin)
Synonyms: murine ribonuclease 5, mR5, Ear5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 54159
VEGA: 14
Homologene: 121614
C2cd6
Name: C2 calcium dependent domain containing 6
Synonyms: 1700052H20Rik, 4930408G06Rik, Als2cr11, Als2cr11b, Gm33589, C2cd6b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 102636554
Homologene: 134310
Prss2
Name: protease, serine 2
Synonyms: TRYP, Ta, Tesp4, TRY8, Try2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22072
HGNC: HGNC:9483
Homologene: 122141
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12041
Homologene: 37642
Tmem88
Name: transmembrane protein 88
Synonyms: 2600017H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67020
Homologene: 12073
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 59,003,232 bp
  • G to T, chromosome 1 at 174,391,429 bp
  • C to A, chromosome 2 at 71,816,681 bp
  • T to C, chromosome 4 at 44,055,273 bp
  • A to T, chromosome 4 at 46,734,119 bp
  • G to A, chromosome 4 at 98,456,169 bp
  • A to G, chromosome 4 at 118,378,064 bp
  • G to A, chromosome 4 at 155,420,267 bp
  • A to T, chromosome 5 at 36,968,309 bp
  • C to T, chromosome 5 at 45,493,557 bp
  • A to G, chromosome 5 at 104,212,840 bp
  • C to A, chromosome 5 at 138,260,929 bp
  • A to T, chromosome 6 at 18,030,209 bp
  • A to T, chromosome 6 at 18,076,493 bp
  • A to T, chromosome 6 at 41,523,976 bp
  • A to T, chromosome 6 at 124,635,089 bp
  • A to T, chromosome 7 at 45,711,013 bp
  • A to G, chromosome 7 at 100,881,781 bp
  • A to G, chromosome 7 at 102,554,944 bp
  • A to T, chromosome 7 at 111,074,198 bp
  • A to C, chromosome 7 at 116,364,503 bp
  • T to C, chromosome 7 at 127,905,740 bp
  • A to G, chromosome 7 at 143,087,738 bp
  • T to A, chromosome 8 at 65,001,323 bp
  • T to A, chromosome 9 at 30,922,137 bp
  • A to T, chromosome 9 at 108,968,875 bp
  • A to G, chromosome 9 at 123,479,499 bp
  • C to T, chromosome 10 at 21,996,601 bp
  • G to T, chromosome 10 at 127,544,516 bp
  • T to C, chromosome 11 at 62,325,601 bp
  • C to A, chromosome 11 at 69,397,776 bp
  • T to C, chromosome 11 at 101,774,237 bp
  • G to A, chromosome 11 at 121,513,125 bp
  • A to C, chromosome 13 at 21,467,437 bp
  • A to G, chromosome 14 at 38,370,814 bp
  • A to G, chromosome 14 at 51,162,890 bp
  • A to T, chromosome 15 at 75,779,309 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • T to C, chromosome 16 at 56,761,873 bp
  • A to T, chromosome 17 at 20,375,467 bp
  • G to A, chromosome 17 at 31,500,610 bp
  • A to G, chromosome 17 at 36,263,754 bp
  • A to T, chromosome 17 at 43,321,221 bp
  • A to G, chromosome 17 at 80,664,088 bp
  • T to C, chromosome 18 at 10,812,118 bp
  • A to C, chromosome 19 at 12,429,955 bp
  • C to A, chromosome 19 at 44,155,964 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2009 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040018-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.