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Strain Name:
C57BL/6J-MtgxR2027Btlr/Mmmh
Stock Number:
040035-MU
Citation ID:
RRID:MMRRC_040035-MU
Other Names:
R2027 (G1), C57BL/6J-MtgxR2027Btlr
Major Collection:

Strain Information

Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381305
Homologene: 19036
Efemp1
Name: epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216616
HGNC: HGNC:3218
Homologene: 3028
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Slc25a13
Name: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
Synonyms: Ctrn, citrin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50799
Homologene: 22800
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 92,573,767 bp
  • C to T, chromosome 1 at 160,954,937 bp
  • A to G, chromosome 2 at 25,938,526 bp
  • A to T, chromosome 2 at 80,535,518 bp
  • A to C, chromosome 2 at 85,918,770 bp
  • T to A, chromosome 2 at 87,071,553 bp
  • A to T, chromosome 2 at 180,689,181 bp
  • T to A, chromosome 3 at 37,047,961 bp
  • G to A, chromosome 3 at 41,730,888 bp
  • T to C, chromosome 3 at 121,463,410 bp
  • T to A, chromosome 4 at 12,171,216 bp
  • T to C, chromosome 4 at 21,958,439 bp
  • T to C, chromosome 4 at 40,941,652 bp
  • T to C, chromosome 4 at 43,495,874 bp
  • A to G, chromosome 4 at 73,403,058 bp
  • T to G, chromosome 4 at 122,862,893 bp
  • C to T, chromosome 4 at 123,371,918 bp
  • A to G, chromosome 4 at 144,271,704 bp
  • A to G, chromosome 4 at 155,735,435 bp
  • T to C, chromosome 5 at 30,421,014 bp
  • T to C, chromosome 5 at 104,327,091 bp
  • A to G, chromosome 5 at 130,208,779 bp
  • A to G, chromosome 6 at 6,073,487 bp
  • A to G, chromosome 6 at 84,584,483 bp
  • C to A, chromosome 6 at 108,386,853 bp
  • A to G, chromosome 6 at 148,877,887 bp
  • C to A, chromosome 7 at 4,993,521 bp
  • G to A, chromosome 7 at 13,131,896 bp
  • C to A, chromosome 7 at 30,315,413 bp
  • G to A, chromosome 7 at 35,454,137 bp
  • C to A, chromosome 7 at 45,708,276 bp
  • A to T, chromosome 7 at 103,041,524 bp
  • T to C, chromosome 7 at 116,350,822 bp
  • T to C, chromosome 7 at 131,320,091 bp
  • T to C, chromosome 8 at 26,038,098 bp
  • T to A, chromosome 8 at 70,177,520 bp
  • A to G, chromosome 8 at 70,515,450 bp
  • G to T, chromosome 8 at 80,695,337 bp
  • A to G, chromosome 8 at 83,671,378 bp
  • A to T, chromosome 8 at 85,072,611 bp
  • A to G, chromosome 8 at 90,907,991 bp
  • T to C, chromosome 8 at 117,187,499 bp
  • C to T, chromosome 9 at 4,317,075 bp
  • T to C, chromosome 9 at 9,005,903 bp
  • G to A, chromosome 9 at 46,243,000 bp
  • T to A, chromosome 9 at 65,078,774 bp
  • T to C, chromosome 9 at 79,645,793 bp
  • A to T, chromosome 9 at 88,731,385 bp
  • T to C, chromosome 9 at 99,620,201 bp
  • G to A, chromosome 9 at 108,947,015 bp
  • T to C, chromosome 10 at 76,038,494 bp
  • A to G, chromosome 10 at 130,162,735 bp
  • A to T, chromosome 11 at 28,914,696 bp
  • T to G, chromosome 11 at 65,955,338 bp
  • A to T, chromosome 11 at 109,957,049 bp
  • A to G, chromosome 11 at 120,009,317 bp
  • A to G, chromosome 13 at 59,742,587 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • C to T, chromosome 13 at 81,595,182 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • G to A, chromosome 14 at 48,256,145 bp
  • A to G, chromosome 14 at 50,237,949 bp
  • A to G, chromosome 14 at 54,908,086 bp
  • T to C, chromosome 14 at 122,921,265 bp
  • A to T, chromosome 15 at 8,157,760 bp
  • A to G, chromosome 15 at 66,917,409 bp
  • A to T, chromosome 15 at 84,701,379 bp
  • A to G, chromosome 16 at 14,232,668 bp
  • T to A, chromosome 17 at 18,929,682 bp
  • A to T, chromosome 17 at 19,522,072 bp
  • A to G, chromosome 17 at 35,982,958 bp
  • G to T, chromosome 17 at 57,839,095 bp
  • A to G, chromosome 18 at 20,583,004 bp
  • A to G, chromosome 18 at 31,984,960 bp
  • T to A, chromosome 19 at 4,087,126 bp
  • A to T, chromosome 19 at 5,089,872 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2027 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040035-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.