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Strain Name:
C57BL/6J-MtgxR2036Btlr/Mmmh
Stock Number:
040043-MU
Citation ID:
RRID:MMRRC_040043-MU
Other Names:
R2036 (G1), C57BL/6J-MtgxR2036Btlr
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Cd40
Name: CD40 antigen
Synonyms: Cd40, Bp50, p50, Tnfrsf5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21939
Homologene: 954
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101476
Homologene: 11001
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Dhdds
Name: dehydrodolichyl diphosphate synthase
Synonyms: 3222401G21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67422
Homologene: 32615
Arhgap35
Name: Rho GTPase activating protein 35
Synonyms: P190 RhoGAP, 6430596G11Rik, p190RhoGAP, p190A, Grlf1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232906
HGNC: HGNC:4591
Homologene: 35136
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 72,666,530 bp
  • A to T, chromosome 1 at 92,596,606 bp
  • A to G, chromosome 1 at 105,743,254 bp
  • A to T, chromosome 1 at 146,701,841 bp
  • C to T, chromosome 2 at 89,001,632 bp
  • T to A, chromosome 2 at 111,449,626 bp
  • T to C, chromosome 2 at 165,062,301 bp
  • C to T, chromosome 2 at 173,101,985 bp
  • T to A, chromosome 2 at 181,237,479 bp
  • A to G, chromosome 3 at 32,472,382 bp
  • A to G, chromosome 4 at 25,620,322 bp
  • A to G, chromosome 4 at 41,632,225 bp
  • G to A, chromosome 4 at 43,568,118 bp
  • C to T, chromosome 4 at 99,218,139 bp
  • T to C, chromosome 4 at 133,971,099 bp
  • A to G, chromosome 5 at 35,716,164 bp
  • C to T, chromosome 5 at 36,952,404 bp
  • A to T, chromosome 5 at 38,752,800 bp
  • T to C, chromosome 5 at 73,022,544 bp
  • C to A, chromosome 5 at 73,107,962 bp
  • T to A, chromosome 5 at 86,309,269 bp
  • C to A, chromosome 5 at 110,656,625 bp
  • G to A, chromosome 5 at 124,304,211 bp
  • C to A, chromosome 5 at 144,828,562 bp
  • T to C, chromosome 6 at 17,660,778 bp
  • C to A, chromosome 6 at 115,611,914 bp
  • A to C, chromosome 7 at 3,156,123 bp
  • A to G, chromosome 7 at 3,685,672 bp
  • T to C, chromosome 7 at 16,563,133 bp
  • A to G, chromosome 7 at 19,293,739 bp
  • A to G, chromosome 7 at 41,863,993 bp
  • G to T, chromosome 7 at 84,856,358 bp
  • T to A, chromosome 7 at 108,367,740 bp
  • T to C, chromosome 7 at 123,318,494 bp
  • A to T, chromosome 7 at 127,030,954 bp
  • A to G, chromosome 7 at 130,902,192 bp
  • A to G, chromosome 8 at 88,591,677 bp
  • T to C, chromosome 9 at 67,272,704 bp
  • T to A, chromosome 9 at 74,151,594 bp
  • T to G, chromosome 9 at 87,042,879 bp
  • T to C, chromosome 10 at 4,407,884 bp
  • A to G, chromosome 10 at 60,466,043 bp
  • T to C, chromosome 10 at 90,969,853 bp
  • G to A, chromosome 10 at 129,333,672 bp
  • A to T, chromosome 11 at 23,276,497 bp
  • A to T, chromosome 11 at 24,164,087 bp
  • T to C, chromosome 11 at 65,041,492 bp
  • G to A, chromosome 11 at 69,497,804 bp
  • G to T, chromosome 11 at 76,452,350 bp
  • C to T, chromosome 11 at 99,685,532 bp
  • C to T, chromosome 11 at 102,576,754 bp
  • T to C, chromosome 13 at 4,576,306 bp
  • A to T, chromosome 14 at 26,608,119 bp
  • A to G, chromosome 14 at 50,928,041 bp
  • A to G, chromosome 16 at 17,303,112 bp
  • T to C, chromosome 18 at 34,628,462 bp
  • G to C, chromosome 18 at 34,738,239 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • T to C, chromosome 19 at 5,568,869 bp
  • C to T, chromosome 19 at 6,213,312 bp
  • T to A, chromosome 19 at 10,610,822 bp
  • G to C, chromosome 19 at 12,680,801 bp
  • T to C, chromosome 19 at 57,731,539 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2036 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040043-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.