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Strain Name:
C57BL/6J-MtgxR2057Btlr/Mmmh
Stock Number:
040062-MU
Citation ID:
RRID:MMRRC_040062-MU
Other Names:
R2057 (G1), C57BL/6J-MtgxR2057Btlr
Major Collection:

Strain Information

Pak1
Name: p21 (RAC1) activated kinase 1
Synonyms: PAK-1, Paka
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18479
HGNC: HGNC:8590
Homologene: 1936
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Cops3
Name: COP9 signalosome subunit 3
Synonyms: Sgn3, COP9 complex S3, Csn3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26572
HGNC: HGNC:2239
Homologene: 2710
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Rarg
Name: retinoic acid receptor, gamma
Synonyms: RARgamma2, RAR gamma 2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19411
HGNC: HGNC:9866
Homologene: 20263
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 21,384,310 bp
  • T to C, chromosome 1 at 63,721,582 bp
  • T to C, chromosome 1 at 139,314,750 bp
  • T to A, chromosome 1 at 150,425,625 bp
  • G to A, chromosome 1 at 158,814,327 bp
  • A to G, chromosome 1 at 171,872,750 bp
  • T to C, chromosome 1 at 194,959,142 bp
  • T to C, chromosome 2 at 5,008,470 bp
  • A to G, chromosome 2 at 5,942,619 bp
  • T to A, chromosome 2 at 25,316,820 bp
  • G to T, chromosome 2 at 34,899,608 bp
  • A to T, chromosome 2 at 37,375,439 bp
  • A to G, chromosome 2 at 40,665,193 bp
  • A to T, chromosome 2 at 53,144,839 bp
  • T to A, chromosome 2 at 87,508,715 bp
  • T to A, chromosome 2 at 160,703,704 bp
  • A to T, chromosome 3 at 31,237,496 bp
  • T to A, chromosome 3 at 75,894,887 bp
  • C to T, chromosome 3 at 108,112,496 bp
  • C to T, chromosome 4 at 11,606,088 bp
  • T to C, chromosome 4 at 33,368,752 bp
  • C to T, chromosome 4 at 126,443,228 bp
  • G to A, chromosome 5 at 24,591,222 bp
  • A to T, chromosome 5 at 121,869,504 bp
  • G to A, chromosome 6 at 41,032,381 bp
  • G to A, chromosome 6 at 48,836,333 bp
  • T to C, chromosome 6 at 58,690,540 bp
  • T to C, chromosome 6 at 72,235,128 bp
  • T to C, chromosome 6 at 72,615,670 bp
  • T to A, chromosome 7 at 26,557,362 bp
  • A to G, chromosome 7 at 42,116,686 bp
  • A to T, chromosome 7 at 59,987,456 bp
  • T to A, chromosome 7 at 97,907,797 bp
  • A to T, chromosome 7 at 99,925,408 bp
  • G to A, chromosome 7 at 131,106,170 bp
  • A to G, chromosome 7 at 144,648,052 bp
  • T to A, chromosome 8 at 22,318,339 bp
  • C to T, chromosome 8 at 22,359,390 bp
  • A to T, chromosome 8 at 24,672,066 bp
  • T to A, chromosome 8 at 63,245,170 bp
  • T to C, chromosome 8 at 85,050,113 bp
  • C to A, chromosome 8 at 104,621,965 bp
  • T to C, chromosome 8 at 111,333,610 bp
  • T to C, chromosome 9 at 44,614,209 bp
  • G to A, chromosome 9 at 100,553,276 bp
  • A to T, chromosome 9 at 109,109,226 bp
  • C to G, chromosome 10 at 24,660,192 bp
  • T to A, chromosome 10 at 45,343,135 bp
  • T to A, chromosome 10 at 88,124,962 bp
  • C to T, chromosome 10 at 97,513,749 bp
  • A to T, chromosome 10 at 130,111,990 bp
  • T to C, chromosome 11 at 50,304,425 bp
  • C to T, chromosome 11 at 50,832,601 bp
  • C to T, chromosome 11 at 55,281,860 bp
  • C to T, chromosome 11 at 59,832,870 bp
  • G to A, chromosome 11 at 67,188,839 bp
  • A to G, chromosome 11 at 74,288,826 bp
  • A to G, chromosome 11 at 102,605,933 bp
  • A to G, chromosome 11 at 106,335,724 bp
  • A to T, chromosome 12 at 8,002,164 bp
  • A to C, chromosome 13 at 13,500,473 bp
  • A to T, chromosome 13 at 33,156,871 bp
  • A to G, chromosome 13 at 38,023,380 bp
  • T to C, chromosome 13 at 41,109,954 bp
  • A to G, chromosome 13 at 54,726,854 bp
  • A to G, chromosome 13 at 93,459,703 bp
  • T to A, chromosome 13 at 100,425,573 bp
  • T to C, chromosome 14 at 26,668,880 bp
  • T to A, chromosome 14 at 29,997,154 bp
  • A to T, chromosome 14 at 32,960,014 bp
  • A to T, chromosome 14 at 33,630,985 bp
  • A to G, chromosome 14 at 101,477,155 bp
  • G to C, chromosome 14 at 101,887,178 bp
  • A to T, chromosome 15 at 36,600,864 bp
  • T to A, chromosome 15 at 37,397,179 bp
  • G to T, chromosome 15 at 83,310,300 bp
  • C to A, chromosome 15 at 102,239,504 bp
  • A to G, chromosome 16 at 9,669,744 bp
  • A to G, chromosome 16 at 15,727,605 bp
  • A to G, chromosome 16 at 18,228,346 bp
  • T to A, chromosome 16 at 93,894,907 bp
  • T to C, chromosome 17 at 22,039,441 bp
  • T to C, chromosome 17 at 23,742,717 bp
  • G to T, chromosome 17 at 24,496,459 bp
  • T to C, chromosome 17 at 25,855,199 bp
  • G to A, chromosome 17 at 31,008,766 bp
  • A to G, chromosome 17 at 31,848,797 bp
  • A to T, chromosome 17 at 34,622,844 bp
  • A to T, chromosome 17 at 34,648,575 bp
  • A to G, chromosome 17 at 34,728,620 bp
  • T to A, chromosome 17 at 35,988,507 bp
  • A to G, chromosome 17 at 43,428,586 bp
  • G to T, chromosome 17 at 46,824,888 bp
  • A to G, chromosome 17 at 50,668,111 bp
  • A to T, chromosome 17 at 52,894,124 bp
  • T to C, chromosome 18 at 5,214,085 bp
  • A to T, chromosome 18 at 46,196,162 bp
  • G to A, chromosome 19 at 4,059,282 bp
  • A to G, chromosome 19 at 43,818,038 bp
  • A to G, chromosome 19 at 47,580,355 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2057 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040062-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.