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Strain Name:
C57BL/6J-MtgxR2075Btlr/Mmmh
Stock Number:
040080-MU
Citation ID:
RRID:MMRRC_040080-MU
Other Names:
R2075 (G1), C57BL/6J-MtgxR2075Btlr
Major Collection:

Strain Information

Esr2
Name: estrogen receptor 2 (beta)
Synonyms: oestrogen receptor beta, ER beta, ERbeta, Estrb
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13983
HGNC: HGNC:3468
Homologene: 1100
Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Atic
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108147
HGNC: HGNC:794
Homologene: 2983
Btbd17
Name: BTB domain containing 17
Synonyms: 1500005I02Rik, Tango10a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72014
Homologene: 46324
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,228,161 bp
  • C to T, chromosome 1 at 60,091,063 bp
  • T to A, chromosome 1 at 71,576,127 bp
  • T to A, chromosome 1 at 150,577,323 bp
  • A to G, chromosome 2 at 26,440,239 bp
  • A to T, chromosome 2 at 67,510,201 bp
  • T to A, chromosome 2 at 82,988,579 bp
  • A to G, chromosome 2 at 89,609,478 bp
  • A to C, chromosome 2 at 101,697,053 bp
  • T to A, chromosome 2 at 106,744,802 bp
  • T to C, chromosome 2 at 111,200,418 bp
  • T to A, chromosome 2 at 122,295,158 bp
  • T to C, chromosome 2 at 130,369,519 bp
  • T to C, chromosome 3 at 39,358,412 bp
  • A to G, chromosome 4 at 32,716,058 bp
  • A to G, chromosome 4 at 42,939,507 bp
  • C to T, chromosome 4 at 48,456,800 bp
  • G to A, chromosome 4 at 63,995,666 bp
  • A to G, chromosome 4 at 94,827,729 bp
  • T to C, chromosome 4 at 96,723,945 bp
  • G to A, chromosome 4 at 103,050,193 bp
  • A to T, chromosome 4 at 154,989,909 bp
  • A to G, chromosome 5 at 35,604,739 bp
  • A to G, chromosome 5 at 52,439,168 bp
  • A to G, chromosome 5 at 69,570,812 bp
  • T to C, chromosome 5 at 75,187,948 bp
  • T to C, chromosome 5 at 120,521,872 bp
  • T to A, chromosome 6 at 95,126,683 bp
  • T to A, chromosome 6 at 124,200,551 bp
  • T to A, chromosome 6 at 124,703,103 bp
  • C to T, chromosome 6 at 129,326,703 bp
  • G to A, chromosome 6 at 140,633,694 bp
  • C to A, chromosome 7 at 4,482,699 bp
  • C to G, chromosome 7 at 30,869,698 bp
  • T to A, chromosome 7 at 103,892,920 bp
  • T to A, chromosome 7 at 104,039,973 bp
  • C to A, chromosome 7 at 127,927,094 bp
  • T to C, chromosome 7 at 133,721,292 bp
  • T to A, chromosome 8 at 33,322,329 bp
  • C to T, chromosome 8 at 70,892,439 bp
  • T to C, chromosome 8 at 80,774,931 bp
  • T to A, chromosome 8 at 93,048,075 bp
  • A to G, chromosome 8 at 94,863,567 bp
  • G to T, chromosome 8 at 95,739,833 bp
  • T to C, chromosome 8 at 109,928,492 bp
  • A to T, chromosome 8 at 123,208,022 bp
  • C to T, chromosome 8 at 123,421,270 bp
  • C to A, chromosome 9 at 22,333,168 bp
  • T to C, chromosome 9 at 50,751,346 bp
  • A to T, chromosome 9 at 53,240,505 bp
  • A to T, chromosome 9 at 75,189,918 bp
  • G to T, chromosome 9 at 78,084,516 bp
  • A to G, chromosome 9 at 102,542,746 bp
  • T to A, chromosome 9 at 123,595,034 bp
  • T to A, chromosome 10 at 19,631,795 bp
  • T to C, chromosome 10 at 52,115,880 bp
  • A to G, chromosome 10 at 62,969,822 bp
  • A to G, chromosome 10 at 77,836,129 bp
  • T to C, chromosome 10 at 127,245,369 bp
  • T to C, chromosome 11 at 9,522,382 bp
  • A to G, chromosome 11 at 16,883,622 bp
  • T to A, chromosome 11 at 75,764,660 bp
  • T to A, chromosome 11 at 106,699,577 bp
  • T to C, chromosome 11 at 106,783,413 bp
  • A to T, chromosome 11 at 114,791,952 bp
  • G to A, chromosome 11 at 120,271,689 bp
  • A to G, chromosome 12 at 76,165,447 bp
  • A to T, chromosome 12 at 85,269,141 bp
  • C to T, chromosome 12 at 102,573,912 bp
  • G to A, chromosome 12 at 117,247,717 bp
  • A to G, chromosome 13 at 6,555,383 bp
  • A to T, chromosome 13 at 22,383,265 bp
  • A to G, chromosome 13 at 42,156,318 bp
  • T to C, chromosome 13 at 55,310,500 bp
  • A to T, chromosome 14 at 32,917,854 bp
  • C to T, chromosome 14 at 51,523,541 bp
  • T to C, chromosome 14 at 63,138,987 bp
  • T to C, chromosome 14 at 77,068,951 bp
  • T to C, chromosome 14 at 78,935,173 bp
  • A to G, chromosome 15 at 12,385,819 bp
  • T to C, chromosome 15 at 34,959,049 bp
  • G to T, chromosome 15 at 44,558,639 bp
  • T to A, chromosome 15 at 59,323,901 bp
  • G to A, chromosome 16 at 10,741,616 bp
  • T to C, chromosome 16 at 59,268,911 bp
  • A to T, chromosome 17 at 22,339,197 bp
  • A to C, chromosome 17 at 22,559,062 bp
  • A to G, chromosome 17 at 23,945,158 bp
  • A to G, chromosome 17 at 85,687,505 bp
  • C to T, chromosome 18 at 12,219,167 bp
  • C to T, chromosome 18 at 63,081,734 bp
  • T to A, chromosome 18 at 67,681,475 bp
  • C to T, chromosome 18 at 73,589,769 bp
  • T to C, chromosome 19 at 7,598,845 bp
  • G to C, chromosome X at 84,312,483 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2075 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040080-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.