Strain Name:
C57BL/6J-MtgxR2079Btlr/Mmmh
Stock Number:
040084-MU
Citation ID:
RRID:MMRRC_040084-MU
Other Names:
R2079 (G1), C57BL/6J-MtgxR2079Btlr
Major Collection:

Gene Information

Gene Symbol: Gulo [MGI:1353434] (Mus musculus (mouse))
Name: gulonolactone (L-) oxidase
Synonyms: sfx, L-gulono-gamma-lactone oxidase
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Kmt2c [MGI:2444959] (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2C
Synonyms: HALR, E330008K23Rik, Mll3
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Pik3cb [MGI:1922019] (Mus musculus (mouse))
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Fancd2 [MGI:2448480] (Mus musculus (mouse))
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Hap1 [MGI:1261831] (Mus musculus (mouse))
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Atad5 [MGI:2442925] (Mus musculus (mouse))
Name: ATPase family, AAA domain containing 5
Synonyms: C130052G03Rik, LOC237877
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Zfp638 [MGI:1203484] (Mus musculus (mouse))
Name: zinc finger protein 638
Synonyms: Zfml, Np220
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 32,467,874 bp
  • T to C, chromosome 1 at 43,764,973 bp
  • G to A, chromosome 1 at 74,917,199 bp
  • A to G, chromosome 1 at 78,511,924 bp
  • A to T, chromosome 1 at 123,432,992 bp
  • A to T, chromosome 1 at 170,004,776 bp
  • A to G, chromosome 1 at 181,817,251 bp
  • A to G, chromosome 2 at 25,900,248 bp
  • A to C, chromosome 2 at 74,744,266 bp
  • G to A, chromosome 2 at 180,225,508 bp
  • T to G, chromosome 3 at 19,644,666 bp
  • G to T, chromosome 3 at 95,879,038 bp
  • A to G, chromosome 3 at 145,007,773 bp
  • C to A, chromosome 4 at 96,531,725 bp
  • T to C, chromosome 4 at 130,055,198 bp
  • T to C, chromosome 4 at 140,933,196 bp
  • T to A, chromosome 4 at 141,991,202 bp
  • T to A, chromosome 4 at 156,331,196 bp
  • C to T, chromosome 5 at 13,451,131 bp
  • T to C, chromosome 5 at 25,352,280 bp
  • T to C, chromosome 5 at 31,251,073 bp
  • T to A, chromosome 5 at 32,946,674 bp
  • C to T, chromosome 5 at 36,814,372 bp
  • T to C, chromosome 5 at 110,244,505 bp
  • A to G, chromosome 5 at 123,818,544 bp
  • T to C, chromosome 5 at 130,190,935 bp
  • A to C, chromosome 5 at 145,029,931 bp
  • A to G, chromosome 5 at 147,355,083 bp
  • T to C, chromosome 6 at 38,818,785 bp
  • T to A, chromosome 6 at 42,520,048 bp
  • T to C, chromosome 6 at 48,690,947 bp
  • T to C, chromosome 6 at 52,620,104 bp
  • T to C, chromosome 6 at 57,955,670 bp
  • T to C, chromosome 6 at 83,953,389 bp
  • T to A, chromosome 6 at 113,425,654 bp
  • T to C, chromosome 6 at 113,555,187 bp
  • A to T, chromosome 6 at 148,789,162 bp
  • A to T, chromosome 7 at 12,193,194 bp
  • G to T, chromosome 7 at 25,307,991 bp
  • T to G, chromosome 7 at 85,957,175 bp
  • T to A, chromosome 7 at 102,949,495 bp
  • A to G, chromosome 7 at 108,925,628 bp
  • T to C, chromosome 7 at 126,796,904 bp
  • C to T, chromosome 7 at 139,944,895 bp
  • A to G, chromosome 8 at 12,857,902 bp
  • C to A, chromosome 8 at 80,615,103 bp
  • T to A, chromosome 8 at 88,141,776 bp
  • T to A, chromosome 8 at 92,850,189 bp
  • A to T, chromosome 9 at 13,244,859 bp
  • C to A, chromosome 9 at 35,223,664 bp
  • C to T, chromosome 9 at 50,907,406 bp
  • T to C, chromosome 9 at 57,089,523 bp
  • T to A, chromosome 9 at 64,153,574 bp
  • G to T, chromosome 9 at 64,153,575 bp
  • A to T, chromosome 9 at 99,060,204 bp
  • G to A, chromosome 9 at 108,859,058 bp
  • A to G, chromosome 10 at 5,361,502 bp
  • A to C, chromosome 10 at 27,369,053 bp
  • A to G, chromosome 10 at 127,720,613 bp
  • T to C, chromosome 10 at 129,012,029 bp
  • G to GGGGT, chromosome 11 at 5,201,794 bp
  • T to G, chromosome 11 at 46,405,107 bp
  • T to C, chromosome 11 at 80,134,200 bp
  • T to C, chromosome 11 at 100,353,746 bp
  • C to T, chromosome 12 at 74,238,299 bp
  • A to G, chromosome 12 at 112,920,377 bp
  • A to T, chromosome 13 at 30,541,308 bp
  • A to T, chromosome 13 at 62,774,610 bp
  • A to T, chromosome 13 at 104,462,238 bp
  • A to G, chromosome 14 at 44,126,254 bp
  • A to T, chromosome 14 at 50,360,980 bp
  • A to G, chromosome 14 at 65,990,383 bp
  • T to C, chromosome 15 at 78,288,007 bp
  • C to T, chromosome 15 at 79,312,994 bp
  • A to T, chromosome 17 at 34,049,844 bp
  • T to C, chromosome 17 at 34,970,220 bp
  • A to T, chromosome 17 at 67,631,880 bp
  • T to A, chromosome 17 at 80,035,377 bp
  • A to G, chromosome 18 at 32,846,973 bp
  • A to T, chromosome 18 at 34,803,517 bp
  • T to A, chromosome 18 at 37,303,309 bp
  • A to G, chromosome 18 at 37,505,171 bp
  • A to G, chromosome 18 at 60,695,509 bp
  • A to G, chromosome 19 at 10,218,673 bp
  • A to T, chromosome 19 at 24,677,653 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2079 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040084-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.