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Strain Name:
C57BL/6J-MtgxR2100Btlr/Mmmh
Stock Number:
040104-MU
Citation ID:
RRID:MMRRC_040104-MU
Other Names:
R2100 (G1), C57BL/6J-MtgxR2100Btlr
Major Collection:

Strain Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Snx10
Name: sorting nexin 10
Synonyms: 2410004M09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71982
Homologene: 32171
Arl5b
Name: ADP-ribosylation factor-like 5B
Synonyms: 4930587A11Rik, Arl8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75869
Homologene: 101695
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,305,271 bp
  • A to G, chromosome 2 at 4,606,023 bp
  • G to A, chromosome 2 at 15,073,195 bp
  • T to C, chromosome 2 at 33,046,645 bp
  • T to A, chromosome 2 at 76,692,663 bp
  • T to C, chromosome 2 at 85,500,249 bp
  • A to T, chromosome 2 at 86,754,561 bp
  • T to A, chromosome 2 at 87,544,825 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • T to A, chromosome 2 at 131,013,679 bp
  • A to G, chromosome 2 at 173,209,244 bp
  • T to C, chromosome 3 at 40,772,658 bp
  • A to G, chromosome 3 at 104,511,630 bp
  • T to C, chromosome 3 at 106,214,347 bp
  • T to C, chromosome 3 at 121,732,433 bp
  • G to A, chromosome 4 at 116,157,967 bp
  • G to A, chromosome 4 at 123,397,906 bp
  • A to T, chromosome 4 at 132,860,602 bp
  • A to T, chromosome 5 at 3,981,110 bp
  • A to G, chromosome 5 at 8,713,202 bp
  • A to G, chromosome 5 at 27,664,744 bp
  • T to G, chromosome 5 at 30,917,897 bp
  • T to A, chromosome 5 at 86,816,685 bp
  • G to T, chromosome 5 at 96,740,680 bp
  • A to G, chromosome 5 at 100,063,786 bp
  • A to G, chromosome 5 at 120,745,675 bp
  • A to T, chromosome 5 at 149,629,438 bp
  • T to A, chromosome 6 at 49,425,681 bp
  • T to C, chromosome 6 at 51,588,415 bp
  • T to C, chromosome 6 at 83,149,560 bp
  • T to A, chromosome 6 at 124,845,042 bp
  • T to A, chromosome 6 at 125,140,822 bp
  • A to G, chromosome 6 at 134,851,203 bp
  • A to G, chromosome 7 at 29,793,526 bp
  • T to C, chromosome 7 at 44,829,497 bp
  • T to A, chromosome 7 at 99,626,444 bp
  • A to T, chromosome 7 at 108,317,554 bp
  • T to G, chromosome 7 at 127,882,187 bp
  • T to C, chromosome 7 at 141,083,292 bp
  • C to T, chromosome 8 at 91,033,987 bp
  • A to T, chromosome 8 at 106,659,668 bp
  • G to T, chromosome 9 at 16,377,430 bp
  • A to G, chromosome 9 at 44,262,608 bp
  • T to C, chromosome 9 at 66,032,359 bp
  • T to A, chromosome 10 at 93,001,937 bp
  • AGGCGG to AGGCGGCGG, chromosome 11 at 5,201,788 bp
  • T to C, chromosome 11 at 69,687,349 bp
  • T to A, chromosome 11 at 70,978,102 bp
  • A to G, chromosome 11 at 74,831,351 bp
  • T to A, chromosome 11 at 82,444,531 bp
  • A to T, chromosome 11 at 109,937,782 bp
  • A to T, chromosome 11 at 119,467,302 bp
  • G to A, chromosome 13 at 23,357,290 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • T to C, chromosome 14 at 8,222,239 bp
  • T to A, chromosome 14 at 18,030,393 bp
  • C to T, chromosome 14 at 19,778,878 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • A to T, chromosome 15 at 44,525,191 bp
  • A to T, chromosome 15 at 83,892,967 bp
  • G to T, chromosome 15 at 98,846,480 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to G, chromosome 16 at 56,231,385 bp
  • C to T, chromosome 17 at 24,408,209 bp
  • T to C, chromosome 17 at 34,916,635 bp
  • G to T, chromosome 18 at 56,708,484 bp
  • A to G, chromosome 19 at 13,530,434 bp
  • A to G, chromosome 19 at 13,815,236 bp
  • C to A, chromosome 19 at 39,886,686 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2100 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040104-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.