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Strain Name:
C57BL/6J-MtgxR2109Btlr/Mmmh
Stock Number:
040113-MU
Citation ID:
RRID:MMRRC_040113-MU
Other Names:
R2109 (G1), C57BL/6J-MtgxR2109Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Hsp90ab1
Name: heat shock protein 90 alpha (cytosolic), class B member 1
Synonyms: Hsp84, Hsp90, Hsp84-1, C81438, Hspcb
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15516
Homologene: 74306
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 93,337,333 bp
  • T to C, chromosome 1 at 189,679,067 bp
  • A to T, chromosome 2 at 20,785,342 bp
  • A to G, chromosome 2 at 25,569,390 bp
  • A to G, chromosome 2 at 26,478,593 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • T to A, chromosome 2 at 76,974,254 bp
  • A to T, chromosome 2 at 125,173,699 bp
  • A to T, chromosome 2 at 178,069,858 bp
  • A to T, chromosome 2 at 181,037,405 bp
  • A to G, chromosome 3 at 9,474,525 bp
  • T to C, chromosome 3 at 33,815,501 bp
  • A to G, chromosome 3 at 90,276,872 bp
  • A to T, chromosome 3 at 93,148,440 bp
  • T to A, chromosome 3 at 94,878,965 bp
  • T to A, chromosome 3 at 98,297,021 bp
  • T to A, chromosome 3 at 135,246,474 bp
  • A to T, chromosome 3 at 138,095,002 bp
  • C to A, chromosome 4 at 46,145,488 bp
  • A to T, chromosome 4 at 52,474,987 bp
  • T to C, chromosome 4 at 55,008,496 bp
  • A to G, chromosome 4 at 58,206,030 bp
  • A to G, chromosome 4 at 115,432,913 bp
  • T to A, chromosome 4 at 132,998,475 bp
  • T to A, chromosome 4 at 139,083,604 bp
  • A to T, chromosome 4 at 144,159,512 bp
  • T to C, chromosome 4 at 149,372,841 bp
  • G to T, chromosome 4 at 154,177,121 bp
  • T to C, chromosome 4 at 154,984,597 bp
  • A to G, chromosome 4 at 156,334,449 bp
  • T to A, chromosome 5 at 4,044,847 bp
  • T to A, chromosome 5 at 25,831,711 bp
  • G to A, chromosome 5 at 30,480,507 bp
  • G to T, chromosome 5 at 121,663,359 bp
  • T to G, chromosome 5 at 144,817,924 bp
  • A to G, chromosome 5 at 150,390,632 bp
  • T to C, chromosome 6 at 3,555,379 bp
  • T to C, chromosome 6 at 124,635,045 bp
  • A to G, chromosome 6 at 140,424,216 bp
  • A to T, chromosome 7 at 19,489,134 bp
  • G to T, chromosome 7 at 44,251,011 bp
  • A to G, chromosome 7 at 47,086,059 bp
  • A to G, chromosome 7 at 48,875,107 bp
  • A to G, chromosome 7 at 82,270,800 bp
  • A to G, chromosome 7 at 105,749,970 bp
  • A to G, chromosome 7 at 109,750,127 bp
  • G to T, chromosome 7 at 110,461,212 bp
  • A to T, chromosome 7 at 119,452,932 bp
  • A to T, chromosome 7 at 126,472,364 bp
  • A to G, chromosome 7 at 134,737,740 bp
  • G to A, chromosome 7 at 135,697,863 bp
  • A to C, chromosome 8 at 48,343,349 bp
  • C to A, chromosome 9 at 42,979,472 bp
  • A to G, chromosome 9 at 54,393,813 bp
  • A to G, chromosome 9 at 64,871,816 bp
  • C to T, chromosome 9 at 85,706,550 bp
  • A to T, chromosome 9 at 94,524,445 bp
  • A to G, chromosome 9 at 95,979,220 bp
  • A to G, chromosome 9 at 120,494,230 bp
  • A to T, chromosome 9 at 121,908,960 bp
  • T to A, chromosome 10 at 21,341,932 bp
  • C to T, chromosome 10 at 83,365,792 bp
  • T to G, chromosome 11 at 45,991,768 bp
  • T to G, chromosome 11 at 49,552,433 bp
  • T to C, chromosome 11 at 50,817,142 bp
  • T to A, chromosome 11 at 51,254,437 bp
  • G to A, chromosome 11 at 55,181,555 bp
  • A to G, chromosome 11 at 55,489,160 bp
  • G to T, chromosome 11 at 66,037,585 bp
  • T to A, chromosome 11 at 69,324,417 bp
  • A to T, chromosome 11 at 69,855,183 bp
  • G to A, chromosome 11 at 114,926,039 bp
  • A to G, chromosome 11 at 115,815,723 bp
  • A to T, chromosome 11 at 116,080,619 bp
  • T to A, chromosome 11 at 119,442,663 bp
  • A to G, chromosome 12 at 32,193,710 bp
  • A to G, chromosome 12 at 34,985,522 bp
  • A to G, chromosome 12 at 55,776,188 bp
  • C to T, chromosome 12 at 81,150,676 bp
  • A to G, chromosome 12 at 111,448,093 bp
  • T to A, chromosome 12 at 116,873,641 bp
  • C to T, chromosome 13 at 13,712,820 bp
  • T to G, chromosome 13 at 104,189,888 bp
  • G to T, chromosome 14 at 61,173,453 bp
  • A to T, chromosome 15 at 36,099,734 bp
  • C to T, chromosome 15 at 66,729,594 bp
  • C to A, chromosome 15 at 76,536,973 bp
  • C to T, chromosome 16 at 5,167,104 bp
  • T to C, chromosome 16 at 15,687,390 bp
  • A to C, chromosome 16 at 78,941,363 bp
  • T to C, chromosome 16 at 87,415,642 bp
  • A to C, chromosome 16 at 90,554,291 bp
  • A to G, chromosome 17 at 6,013,691 bp
  • G to T, chromosome 17 at 33,019,778 bp
  • C to T, chromosome 17 at 34,379,604 bp
  • G to T, chromosome 17 at 45,569,328 bp
  • A to T, chromosome 17 at 55,653,413 bp
  • T to G, chromosome 18 at 12,759,400 bp
  • A to G, chromosome 18 at 20,592,289 bp
  • C to T, chromosome 18 at 88,986,073 bp
  • A to T, chromosome 19 at 4,838,816 bp
  • T to A, chromosome 19 at 6,014,358 bp
  • T to A, chromosome 19 at 18,641,438 bp
  • C to T, chromosome 19 at 50,678,192 bp
  • A to G, chromosome 19 at 61,069,403 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2109 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040113-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.