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Strain Name:
C57BL/6J-MtgxR2111Btlr/Mmmh
Stock Number:
040115-MU
Citation ID:
RRID:MMRRC_040115-MU
Other Names:
R2111 (G1), C57BL/6J-MtgxR2111Btlr
Major Collection:

Strain Information

Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Tagln3
Name: transgelin 3
Synonyms: 2900005O10Rik, 2700038H05Rik, Np25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56370
Homologene: 22337
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,169,178 bp
  • T to A, chromosome 1 at 67,176,980 bp
  • T to G, chromosome 1 at 83,275,881 bp
  • A to T, chromosome 1 at 87,126,885 bp
  • A to G, chromosome 1 at 87,440,730 bp
  • A to G, chromosome 1 at 87,950,214 bp
  • C to T, chromosome 1 at 135,449,004 bp
  • T to A, chromosome 1 at 139,774,679 bp
  • A to T, chromosome 1 at 173,270,312 bp
  • A to T, chromosome 2 at 25,437,505 bp
  • G to T, chromosome 2 at 34,684,317 bp
  • A to G, chromosome 2 at 52,284,263 bp
  • A to G, chromosome 2 at 53,105,537 bp
  • A to T, chromosome 2 at 74,763,366 bp
  • G to T, chromosome 2 at 86,658,437 bp
  • A to G, chromosome 2 at 88,224,474 bp
  • A to G, chromosome 2 at 121,954,825 bp
  • G to A, chromosome 2 at 156,748,017 bp
  • A to G, chromosome 3 at 87,977,311 bp
  • A to G, chromosome 4 at 32,700,409 bp
  • A to G, chromosome 4 at 96,728,334 bp
  • T to C, chromosome 4 at 135,155,316 bp
  • G to A, chromosome 5 at 31,286,079 bp
  • T to C, chromosome 5 at 87,092,241 bp
  • T to C, chromosome 5 at 97,456,305 bp
  • A to G, chromosome 5 at 122,459,546 bp
  • T to C, chromosome 5 at 128,773,501 bp
  • C to T, chromosome 5 at 136,030,577 bp
  • C to A, chromosome 6 at 68,161,629 bp
  • C to A, chromosome 6 at 81,923,778 bp
  • T to G, chromosome 6 at 108,378,309 bp
  • T to C, chromosome 6 at 116,540,650 bp
  • T to C, chromosome 6 at 127,015,760 bp
  • A to G, chromosome 7 at 4,445,483 bp
  • G to A, chromosome 7 at 29,935,018 bp
  • C to A, chromosome 7 at 140,773,634 bp
  • A to G, chromosome 8 at 3,169,748 bp
  • A to T, chromosome 8 at 13,001,867 bp
  • A to G, chromosome 8 at 13,248,694 bp
  • C to T, chromosome 8 at 24,982,126 bp
  • G to T, chromosome 8 at 41,022,571 bp
  • T to A, chromosome 8 at 42,082,968 bp
  • T to A, chromosome 8 at 61,124,326 bp
  • A to T, chromosome 8 at 70,889,751 bp
  • T to A, chromosome 8 at 72,427,268 bp
  • A to T, chromosome 8 at 77,667,979 bp
  • T to C, chromosome 8 at 105,614,712 bp
  • T to A, chromosome 9 at 38,539,280 bp
  • T to C, chromosome 9 at 40,282,445 bp
  • A to G, chromosome 9 at 75,509,363 bp
  • C to A, chromosome 9 at 96,039,832 bp
  • C to T, chromosome 10 at 7,793,034 bp
  • G to T, chromosome 10 at 60,305,583 bp
  • T to C, chromosome 10 at 76,420,526 bp
  • G to T, chromosome 10 at 79,612,421 bp
  • G to A, chromosome 10 at 79,976,785 bp
  • A to C, chromosome 10 at 115,240,914 bp
  • T to C, chromosome 11 at 3,432,393 bp
  • G to A, chromosome 11 at 60,388,946 bp
  • G to A, chromosome 11 at 67,214,620 bp
  • A to G, chromosome 11 at 73,964,914 bp
  • G to A, chromosome 11 at 82,911,689 bp
  • C to A, chromosome 12 at 18,853,894 bp
  • A to T, chromosome 12 at 35,138,085 bp
  • T to A, chromosome 12 at 54,911,385 bp
  • T to C, chromosome 12 at 103,407,589 bp
  • G to A, chromosome 12 at 104,116,222 bp
  • C to T, chromosome 12 at 113,131,628 bp
  • A to T, chromosome 13 at 12,244,601 bp
  • G to A, chromosome 13 at 19,116,266 bp
  • A to G, chromosome 13 at 34,990,716 bp
  • A to G, chromosome 13 at 46,683,928 bp
  • A to G, chromosome 13 at 63,834,749 bp
  • T to C, chromosome 13 at 65,199,353 bp
  • C to T, chromosome 13 at 73,785,155 bp
  • T to A, chromosome 13 at 89,693,303 bp
  • A to T, chromosome 13 at 107,769,723 bp
  • T to C, chromosome 14 at 30,519,370 bp
  • A to G, chromosome 15 at 8,121,467 bp
  • A to T, chromosome 15 at 9,589,573 bp
  • A to G, chromosome 15 at 57,932,644 bp
  • C to T, chromosome 15 at 69,024,824 bp
  • C to T, chromosome 15 at 78,847,492 bp
  • G to A, chromosome 16 at 13,603,069 bp
  • A to G, chromosome 16 at 36,721,979 bp
  • A to G, chromosome 16 at 37,978,751 bp
  • A to G, chromosome 16 at 45,711,594 bp
  • A to G, chromosome 17 at 6,956,860 bp
  • A to G, chromosome 17 at 46,963,145 bp
  • T to A, chromosome 17 at 64,290,036 bp
  • T to C, chromosome 17 at 75,500,758 bp
  • T to C, chromosome 17 at 84,577,329 bp
  • T to C, chromosome 18 at 65,349,774 bp
  • G to A, chromosome 19 at 13,410,943 bp
  • T to C, chromosome 19 at 17,208,238 bp
  • T to A, chromosome X at 100,614,641 bp
  • A to T, chromosome X at 101,407,387 bp
  • A to G, chromosome X at 150,997,294 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2111 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040115-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.