Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR2120Btlr/Mmmh
Stock Number:
040124-MU
Citation ID:
RRID:MMRRC_040124-MU
Other Names:
R2120 (G1), C57BL/6J-MtgxR2120Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Rasgrp2 [MGI:1333849] (Mus musculus (mouse))
Name: RAS, guanyl releasing protein 2
Synonyms: Caldaggef1, CalDAG-GEFI
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Jarid2 [MGI:104813] (Mus musculus (mouse))
Name: jumonji, AT rich interactive domain 2
Synonyms: jumonji, Jmj
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Pum1 [MGI:1931749] (Mus musculus (mouse))
Name: pumilio RNA-binding family member 1
Synonyms: Pumm
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Fgd4 [MGI:2183747] (Mus musculus (mouse))
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin, ZFYVE6, Frabin-gamma, Frabin-beta, Frabin-alpha, 9330209B17Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef10 [MGI:2444453] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF) 10
Synonyms: 6430549H08Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Bicral [MGI:2673855] (Mus musculus (mouse))
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Chromosome: 17
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,715,228 bp
  • A to G, chromosome 1 at 74,564,425 bp
  • T to C, chromosome 1 at 75,220,460 bp
  • T to A, chromosome 1 at 86,078,700 bp
  • A to G, chromosome 1 at 86,545,802 bp
  • A to G, chromosome 1 at 87,148,275 bp
  • G to T, chromosome 1 at 93,402,133 bp
  • C to T, chromosome 1 at 97,066,083 bp
  • A to G, chromosome 1 at 107,605,887 bp
  • G to A, chromosome 1 at 132,516,512 bp
  • A to G, chromosome 1 at 182,441,639 bp
  • A to T, chromosome 1 at 185,261,367 bp
  • T to A, chromosome 2 at 6,440,937 bp
  • C to T, chromosome 2 at 49,547,609 bp
  • A to G, chromosome 2 at 52,264,064 bp
  • C to T, chromosome 2 at 66,226,754 bp
  • G to A, chromosome 2 at 86,333,345 bp
  • A to G, chromosome 2 at 111,315,499 bp
  • T to A, chromosome 2 at 122,003,892 bp
  • T to C, chromosome 2 at 157,033,325 bp
  • A to T, chromosome 2 at 168,653,537 bp
  • T to C, chromosome 3 at 108,434,156 bp
  • T to A, chromosome 4 at 98,456,225 bp
  • C to T, chromosome 4 at 108,023,361 bp
  • T to A, chromosome 4 at 123,824,919 bp
  • C to T, chromosome 4 at 126,128,847 bp
  • G to A, chromosome 4 at 126,152,330 bp
  • C to A, chromosome 4 at 130,669,270 bp
  • C to T, chromosome 4 at 154,667,094 bp
  • A to G, chromosome 4 at 155,792,073 bp
  • G to A, chromosome 5 at 21,969,085 bp
  • G to A, chromosome 5 at 124,127,269 bp
  • A to G, chromosome 5 at 128,788,518 bp
  • G to A, chromosome 6 at 40,515,360 bp
  • T to C, chromosome 6 at 113,371,015 bp
  • A to T, chromosome 6 at 116,540,455 bp
  • C to T, chromosome 6 at 124,508,713 bp
  • A to G, chromosome 6 at 125,309,477 bp
  • C to T, chromosome 6 at 126,934,702 bp
  • A to T, chromosome 6 at 131,678,354 bp
  • T to A, chromosome 6 at 132,737,507 bp
  • T to C, chromosome 7 at 5,053,009 bp
  • T to A, chromosome 7 at 18,681,022 bp
  • A to G, chromosome 7 at 27,036,646 bp
  • T to C, chromosome 7 at 80,340,098 bp
  • G to T, chromosome 7 at 110,049,631 bp
  • A to G, chromosome 7 at 120,792,418 bp
  • T to C, chromosome 7 at 140,926,444 bp
  • A to G, chromosome 8 at 14,934,820 bp
  • T to C, chromosome 8 at 22,667,217 bp
  • A to T, chromosome 8 at 80,615,457 bp
  • A to G, chromosome 8 at 85,093,024 bp
  • A to G, chromosome 8 at 105,300,341 bp
  • T to C, chromosome 9 at 14,357,653 bp
  • C to T, chromosome 9 at 37,522,931 bp
  • T to A, chromosome 9 at 45,101,064 bp
  • C to A, chromosome 9 at 57,447,225 bp
  • A to G, chromosome 9 at 67,919,334 bp
  • A to T, chromosome 9 at 73,578,505 bp
  • A to G, chromosome 9 at 110,549,864 bp
  • T to A, chromosome 9 at 120,051,683 bp
  • T to A, chromosome 9 at 121,472,997 bp
  • A to T, chromosome 10 at 12,699,125 bp
  • T to A, chromosome 10 at 62,507,634 bp
  • A to T, chromosome 10 at 79,903,082 bp
  • T to A, chromosome 10 at 82,887,233 bp
  • T to C, chromosome 11 at 4,269,051 bp
  • T to C, chromosome 11 at 9,309,013 bp
  • A to C, chromosome 11 at 33,118,003 bp
  • T to C, chromosome 11 at 99,321,197 bp
  • T to C, chromosome 11 at 113,649,598 bp
  • C to T, chromosome 11 at 117,148,532 bp
  • A to T, chromosome 12 at 29,783,619 bp
  • T to C, chromosome 12 at 101,147,697 bp
  • G to T, chromosome 13 at 44,906,336 bp
  • T to C, chromosome 14 at 50,533,946 bp
  • A to T, chromosome 14 at 51,413,208 bp
  • T to C, chromosome 14 at 60,296,659 bp
  • C to T, chromosome 15 at 11,310,579 bp
  • C to A, chromosome 15 at 89,007,627 bp
  • C to T, chromosome 15 at 89,790,776 bp
  • A to G, chromosome 15 at 98,839,529 bp
  • C to T, chromosome 16 at 16,425,828 bp
  • G to T, chromosome 16 at 95,718,933 bp
  • T to G, chromosome 17 at 23,359,323 bp
  • T to G, chromosome 17 at 36,670,037 bp
  • C to T, chromosome 17 at 46,824,815 bp
  • T to A, chromosome 17 at 75,310,159 bp
  • T to A, chromosome 17 at 84,671,147 bp
  • A to T, chromosome 18 at 34,276,601 bp
  • T to A, chromosome 19 at 6,404,395 bp
  • G to C, chromosome X at 84,312,483 bp
Genotype Determination
ES Cell Line
N/A

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice

    Strain Origin

    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R2120 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

    Appearance

    Coat Color
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

    Reproductive Statistics

    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    040124-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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