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Strain Name:
C57BL/6J-MtgxR2129Btlr/Mmmh
Stock Number:
040132-MU
Citation ID:
RRID:MMRRC_040132-MU
Other Names:
R2129 (G1), C57BL/6J-MtgxR2129Btlr
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Gpr6
Name: G protein-coupled receptor 6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 140741
VEGA: 10
HGNC: HGNC:4515
Homologene: 38026
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 38,865,695 bp
  • A to G, chromosome 1 at 78,196,658 bp
  • T to C, chromosome 1 at 139,457,635 bp
  • A to T, chromosome 1 at 160,075,375 bp
  • A to T, chromosome 1 at 160,738,957 bp
  • T to C, chromosome 1 at 163,966,457 bp
  • A to G, chromosome 1 at 180,965,540 bp
  • A to T, chromosome 2 at 16,512,091 bp
  • G to A, chromosome 2 at 26,846,574 bp
  • A to G, chromosome 2 at 104,728,173 bp
  • A to G, chromosome 2 at 112,678,370 bp
  • A to T, chromosome 2 at 120,182,811 bp
  • T to C, chromosome 2 at 120,942,553 bp
  • T to C, chromosome 2 at 140,048,244 bp
  • C to A, chromosome 3 at 53,477,945 bp
  • A to T, chromosome 3 at 87,758,359 bp
  • T to A, chromosome 3 at 89,357,545 bp
  • A to T, chromosome 3 at 134,854,860 bp
  • T to C, chromosome 3 at 145,016,765 bp
  • T to G, chromosome 4 at 21,900,342 bp
  • G to T, chromosome 4 at 24,898,608 bp
  • T to C, chromosome 4 at 32,640,142 bp
  • T to A, chromosome 4 at 41,098,061 bp
  • C to T, chromosome 4 at 56,977,010 bp
  • A to G, chromosome 4 at 123,368,815 bp
  • T to C, chromosome 4 at 132,719,197 bp
  • T to A, chromosome 4 at 134,018,154 bp
  • G to A, chromosome 4 at 141,017,096 bp
  • G to A, chromosome 4 at 149,187,640 bp
  • G to A, chromosome 4 at 155,860,470 bp
  • G to T, chromosome 5 at 32,831,828 bp
  • A to G, chromosome 5 at 65,594,926 bp
  • A to T, chromosome 5 at 89,568,065 bp
  • A to T, chromosome 5 at 99,234,466 bp
  • A to G, chromosome 5 at 103,609,056 bp
  • A to T, chromosome 5 at 112,831,078 bp
  • A to G, chromosome 5 at 114,111,163 bp
  • T to A, chromosome 5 at 125,451,671 bp
  • C to A, chromosome 5 at 142,435,188 bp
  • A to G, chromosome 6 at 29,757,314 bp
  • A to C, chromosome 6 at 131,168,284 bp
  • T to C, chromosome 7 at 25,330,715 bp
  • T to C, chromosome 7 at 30,591,493 bp
  • T to G, chromosome 7 at 45,282,773 bp
  • T to A, chromosome 7 at 108,055,904 bp
  • C to T, chromosome 8 at 54,632,381 bp
  • T to C, chromosome 8 at 61,877,361 bp
  • T to C, chromosome 8 at 71,333,699 bp
  • T to A, chromosome 8 at 104,548,973 bp
  • G to A, chromosome 8 at 105,138,097 bp
  • T to C, chromosome 8 at 105,385,172 bp
  • A to G, chromosome 9 at 7,175,289 bp
  • T to C, chromosome 9 at 30,904,503 bp
  • C to T, chromosome 9 at 54,714,255 bp
  • T to G, chromosome 9 at 64,905,974 bp
  • A to T, chromosome 9 at 69,476,128 bp
  • T to A, chromosome 9 at 95,561,069 bp
  • A to T, chromosome 9 at 106,440,865 bp
  • T to A, chromosome 9 at 121,152,182 bp
  • G to A, chromosome 10 at 41,071,172 bp
  • A to G, chromosome 10 at 61,348,306 bp
  • A to G, chromosome 10 at 76,457,515 bp
  • A to G, chromosome 10 at 79,665,572 bp
  • T to C, chromosome 10 at 86,695,706 bp
  • A to G, chromosome 10 at 87,230,204 bp
  • T to G, chromosome 10 at 88,551,452 bp
  • A to G, chromosome 10 at 88,814,055 bp
  • G to A, chromosome 10 at 103,214,857 bp
  • A to G, chromosome 10 at 109,767,648 bp
  • C to A, chromosome 11 at 4,888,932 bp
  • T to C, chromosome 11 at 34,727,415 bp
  • T to C, chromosome 11 at 58,529,611 bp
  • G to T, chromosome 11 at 98,327,588 bp
  • A to T, chromosome 11 at 115,176,444 bp
  • A to G, chromosome 11 at 116,649,842 bp
  • A to G, chromosome 12 at 100,678,538 bp
  • G to A, chromosome 12 at 104,722,031 bp
  • T to C, chromosome 13 at 22,620,612 bp
  • A to T, chromosome 13 at 35,898,621 bp
  • A to G, chromosome 13 at 76,824,822 bp
  • A to T, chromosome 13 at 81,557,080 bp
  • T to C, chromosome 13 at 117,267,546 bp
  • C to A, chromosome 14 at 27,000,845 bp
  • A to G, chromosome 14 at 50,710,636 bp
  • T to A, chromosome 14 at 64,028,966 bp
  • G to T, chromosome 15 at 6,336,383 bp
  • T to C, chromosome 15 at 25,781,799 bp
  • A to G, chromosome 15 at 28,408,321 bp
  • A to G, chromosome 15 at 66,694,894 bp
  • T to A, chromosome 15 at 73,756,790 bp
  • T to C, chromosome 16 at 21,946,161 bp
  • C to T, chromosome 16 at 23,927,707 bp
  • T to C, chromosome 16 at 88,877,975 bp
  • G to C, chromosome 17 at 35,039,421 bp
  • G to A, chromosome 17 at 46,188,088 bp
  • T to C, chromosome 17 at 80,183,218 bp
  • T to A, chromosome 17 at 83,425,243 bp
  • T to A, chromosome 17 at 86,496,035 bp
  • A to T, chromosome 18 at 5,767,681 bp
  • A to G, chromosome 18 at 46,465,664 bp
  • A to T, chromosome 19 at 20,795,506 bp
  • T to A, chromosome 19 at 21,681,049 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2129 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040132-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.