Strain Name:
C57BL/6J-MtgxR2138Btlr/Mmmh
Stock Number:
040141-MU
Citation ID:
RRID:MMRRC_040141-MU
Other Names:
R2138 (G1), C57BL/6J-MtgxR2138Btlr
Major Collection:

Gene Information

Gene Symbol: Wasf2 [MGI:1098641] (Mus musculus (mouse))
Name: WAS protein family, member 2
Synonyms: D4Ertd13e, WAVE2
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Tln2 [MGI:1917799] (Mus musculus (mouse))
Name: talin 2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Baiap2 [MGI:2137336] (Mus musculus (mouse))
Name: brain-specific angiogenesis inhibitor 1-associated protein 2
Synonyms: IRSp53
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Lamb3 [MGI:99915] (Mus musculus (mouse))
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ptprf [MGI:102695] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: RPTP-LAR, LAR
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Bcar3 [MGI:1352501] (Mus musculus (mouse))
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Exoc6b [MGI:1923164] (Mus musculus (mouse))
Name: exocyst complex component 6B
Synonyms: 4930569O18Rik, Sec15l2, G430127E12Rik, Sec15b
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 72,300,996 bp
  • A to G, chromosome 1 at 74,283,963 bp
  • G to T, chromosome 1 at 98,443,660 bp
  • A to G, chromosome 1 at 105,640,729 bp
  • A to G, chromosome 1 at 135,607,870 bp
  • G to A, chromosome 1 at 151,696,251 bp
  • G to A, chromosome 1 at 170,880,597 bp
  • A to T, chromosome 1 at 174,250,736 bp
  • T to A, chromosome 1 at 193,331,420 bp
  • A to G, chromosome 2 at 13,444,378 bp
  • A to T, chromosome 2 at 52,052,332 bp
  • G to T, chromosome 2 at 164,952,467 bp
  • G to T, chromosome 3 at 89,446,184 bp
  • T to A, chromosome 3 at 90,352,113 bp
  • T to C, chromosome 3 at 104,804,242 bp
  • A to G, chromosome 3 at 122,512,996 bp
  • A to T, chromosome 3 at 124,412,380 bp
  • G to T, chromosome 4 at 106,572,302 bp
  • A to T, chromosome 4 at 118,238,023 bp
  • T to C, chromosome 4 at 133,192,101 bp
  • A to T, chromosome 4 at 141,250,800 bp
  • A to T, chromosome 4 at 149,718,080 bp
  • A to T, chromosome 5 at 11,419,085 bp
  • A to G, chromosome 5 at 30,461,770 bp
  • T to C, chromosome 5 at 87,676,983 bp
  • A to G, chromosome 5 at 90,499,647 bp
  • C to T, chromosome 5 at 108,606,877 bp
  • A to T, chromosome 5 at 143,324,262 bp
  • A to T, chromosome 6 at 12,471,073 bp
  • C to T, chromosome 6 at 40,756,450 bp
  • G to A, chromosome 6 at 64,345,798 bp
  • A to T, chromosome 6 at 84,989,482 bp
  • A to T, chromosome 6 at 91,498,122 bp
  • C to A, chromosome 6 at 110,646,137 bp
  • A to T, chromosome 6 at 113,796,307 bp
  • G to T, chromosome 6 at 116,305,604 bp
  • A to G, chromosome 6 at 123,023,978 bp
  • A to T, chromosome 6 at 130,333,158 bp
  • G to A, chromosome 7 at 19,917,002 bp
  • A to G, chromosome 7 at 45,981,051 bp
  • T to C, chromosome 8 at 15,929,088 bp
  • A to T, chromosome 9 at 18,444,958 bp
  • A to G, chromosome 9 at 64,889,337 bp
  • G to A, chromosome 9 at 66,470,307 bp
  • A to G, chromosome 9 at 67,221,527 bp
  • A to G, chromosome 9 at 104,025,903 bp
  • C to A, chromosome 9 at 104,032,069 bp
  • A to G, chromosome 9 at 115,232,134 bp
  • T to A, chromosome 10 at 19,632,870 bp
  • C to A, chromosome 10 at 39,147,351 bp
  • A to T, chromosome 10 at 41,581,297 bp
  • C to T, chromosome 10 at 61,179,284 bp
  • T to C, chromosome 10 at 79,756,601 bp
  • C to A, chromosome 10 at 80,527,105 bp
  • A to T, chromosome 10 at 97,682,270 bp
  • G to A, chromosome 10 at 116,795,538 bp
  • G to A, chromosome 11 at 35,841,887 bp
  • A to G, chromosome 11 at 43,601,434 bp
  • T to G, chromosome 11 at 53,372,512 bp
  • A to T, chromosome 11 at 59,003,665 bp
  • T to G, chromosome 11 at 69,805,114 bp
  • T to A, chromosome 11 at 73,165,618 bp
  • A to T, chromosome 11 at 73,984,303 bp
  • A to T, chromosome 11 at 94,648,192 bp
  • T to C, chromosome 11 at 115,883,807 bp
  • A to G, chromosome 11 at 119,957,102 bp
  • A to T, chromosome 12 at 11,241,621 bp
  • C to A, chromosome 12 at 32,838,422 bp
  • C to T, chromosome 12 at 101,761,920 bp
  • A to T, chromosome 13 at 24,818,205 bp
  • T to C, chromosome 13 at 81,445,320 bp
  • A to T, chromosome 13 at 92,439,406 bp
  • A to G, chromosome 14 at 7,923,560 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • T to A, chromosome 14 at 41,153,691 bp
  • T to C, chromosome 14 at 50,963,704 bp
  • T to C, chromosome 14 at 62,361,345 bp
  • A to C, chromosome 15 at 44,501,457 bp
  • G to T, chromosome 15 at 78,674,038 bp
  • C to T, chromosome 16 at 4,992,206 bp
  • A to G, chromosome 16 at 16,673,205 bp
  • A to G, chromosome 16 at 20,644,439 bp
  • A to G, chromosome 16 at 59,622,467 bp
  • T to C, chromosome 16 at 91,659,372 bp
  • A to G, chromosome 17 at 69,207,880 bp
  • G to T, chromosome 17 at 80,263,933 bp
  • A to G, chromosome 18 at 31,955,407 bp
  • A to G, chromosome 19 at 5,496,129 bp
  • A to T, chromosome 19 at 10,997,331 bp
  • G to A, chromosome 19 at 25,411,753 bp
  • T to A, chromosome 19 at 56,842,589 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2138 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040141-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.