Strain Name:
C57BL/6J-MtgxR2144Btlr/Mmmh
Stock Number:
040147-MU
Citation ID:
RRID:MMRRC_040147-MU
Other Names:
R2144 (G1), C57BL/6J-MtgxR2144Btlr
Major Collection:

Gene Information

Gene Symbol: Npy1r [MGI:104963] (Mus musculus (mouse))
Name: neuropeptide Y receptor Y1
Synonyms: Npyr, Y1-R
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Ep400 [MGI:1276124] (Mus musculus (mouse))
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, mDomino, p400
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Kansl2 [MGI:1916862] (Mus musculus (mouse))
Name: KAT8 regulatory NSL complex subunit 2
Synonyms: 2310037I24Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Rtel1 [MGI:2139369] (Mus musculus (mouse))
Name: regulator of telomere elongation helicase 1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Pepd [MGI:97542] (Mus musculus (mouse))
Name: peptidase D
Synonyms: dal, Pep4, Pep-4, peptidase D
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Osbpl1a [MGI:1927551] (Mus musculus (mouse))
Name: oxysterol binding protein-like 1A
Synonyms: G430090F17Rik, LOC328902
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Nphs1 [MGI:1859637] (Mus musculus (mouse))
Name: nephrosis 1, nephrin
Synonyms: nephrin
Chromosome: 7
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,746,831 bp
  • C to A, chromosome 1 at 75,363,464 bp
  • A to G, chromosome 1 at 75,366,804 bp
  • T to A, chromosome 1 at 93,271,684 bp
  • C to T, chromosome 1 at 93,321,297 bp
  • C to T, chromosome 1 at 116,101,710 bp
  • T to C, chromosome 1 at 130,698,723 bp
  • C to A, chromosome 1 at 131,273,474 bp
  • T to A, chromosome 1 at 132,463,375 bp
  • A to T, chromosome 1 at 191,061,267 bp
  • A to T, chromosome 2 at 5,810,806 bp
  • T to C, chromosome 2 at 37,518,231 bp
  • G to A, chromosome 2 at 49,728,575 bp
  • A to G, chromosome 2 at 111,873,423 bp
  • A to G, chromosome 2 at 130,397,410 bp
  • C to A, chromosome 2 at 146,388,604 bp
  • A to G, chromosome 2 at 153,003,007 bp
  • T to A, chromosome 2 at 181,323,706 bp
  • T to C, chromosome 4 at 58,812,632 bp
  • T to A, chromosome 4 at 65,180,949 bp
  • G to A, chromosome 4 at 88,868,174 bp
  • T to A, chromosome 4 at 88,868,175 bp
  • G to T, chromosome 4 at 108,625,087 bp
  • T to C, chromosome 4 at 113,236,260 bp
  • T to A, chromosome 4 at 114,228,885 bp
  • T to C, chromosome 4 at 134,093,737 bp
  • C to A, chromosome 4 at 134,371,044 bp
  • T to C, chromosome 4 at 134,580,767 bp
  • T to C, chromosome 4 at 150,616,931 bp
  • G to A, chromosome 4 at 155,847,816 bp
  • C to T, chromosome 5 at 14,858,752 bp
  • T to A, chromosome 5 at 22,547,927 bp
  • A to T, chromosome 5 at 24,100,863 bp
  • A to G, chromosome 5 at 31,154,189 bp
  • A to T, chromosome 5 at 88,328,395 bp
  • A to T, chromosome 5 at 110,703,518 bp
  • G to T, chromosome 5 at 145,178,762 bp
  • A to G, chromosome 5 at 145,456,084 bp
  • A to C, chromosome 5 at 149,630,337 bp
  • A to T, chromosome 6 at 17,886,007 bp
  • A to T, chromosome 6 at 42,642,804 bp
  • A to G, chromosome 6 at 48,975,291 bp
  • G to A, chromosome 6 at 60,945,075 bp
  • A to G, chromosome 6 at 106,778,039 bp
  • A to G, chromosome 6 at 119,948,988 bp
  • A to G, chromosome 6 at 122,315,367 bp
  • A to G, chromosome 6 at 125,174,372 bp
  • T to C, chromosome 6 at 141,490,111 bp
  • T to C, chromosome 6 at 141,809,378 bp
  • A to T, chromosome 7 at 3,844,345 bp
  • A to C, chromosome 7 at 10,381,811 bp
  • A to T, chromosome 7 at 27,034,769 bp
  • C to A, chromosome 7 at 28,541,069 bp
  • A to G, chromosome 7 at 30,105,340 bp
  • A to G, chromosome 7 at 30,460,970 bp
  • A to G, chromosome 7 at 30,641,110 bp
  • G to T, chromosome 7 at 31,291,763 bp
  • A to T, chromosome 7 at 34,921,418 bp
  • A to G, chromosome 7 at 45,887,040 bp
  • T to C, chromosome 7 at 47,235,463 bp
  • G to A, chromosome 7 at 65,655,791 bp
  • G to A, chromosome 7 at 66,296,163 bp
  • A to G, chromosome 7 at 80,363,516 bp
  • A to G, chromosome 7 at 81,038,202 bp
  • T to A, chromosome 7 at 86,695,280 bp
  • C to T, chromosome 7 at 86,954,482 bp
  • A to T, chromosome 7 at 106,688,957 bp
  • A to T, chromosome 7 at 109,475,113 bp
  • T to G, chromosome 8 at 3,622,355 bp
  • C to A, chromosome 8 at 24,815,480 bp
  • A to T, chromosome 8 at 46,586,343 bp
  • T to A, chromosome 8 at 66,705,184 bp
  • A to G, chromosome 8 at 68,796,805 bp
  • T to C, chromosome 8 at 72,731,265 bp
  • A to G, chromosome 8 at 75,069,088 bp
  • A to G, chromosome 8 at 111,118,036 bp
  • C to T, chromosome 9 at 38,848,339 bp
  • T to C, chromosome 9 at 96,854,492 bp
  • T to C, chromosome 9 at 107,913,168 bp
  • T to C, chromosome 10 at 53,358,126 bp
  • T to C, chromosome 10 at 84,613,499 bp
  • C to T, chromosome 10 at 88,428,506 bp
  • G to A, chromosome 10 at 127,028,445 bp
  • A to T, chromosome 11 at 54,341,778 bp
  • T to A, chromosome 11 at 68,543,611 bp
  • A to G, chromosome 11 at 70,671,575 bp
  • G to A, chromosome 11 at 74,425,976 bp
  • A to T, chromosome 11 at 75,647,191 bp
  • T to G, chromosome 11 at 95,385,914 bp
  • T to C, chromosome 11 at 97,934,012 bp
  • A to T, chromosome 11 at 98,896,214 bp
  • T to C, chromosome 11 at 99,580,818 bp
  • A to T, chromosome 11 at 99,792,963 bp
  • T to C, chromosome 11 at 119,443,690 bp
  • A to G, chromosome 12 at 69,741,054 bp
  • A to C, chromosome 12 at 98,810,605 bp
  • T to A, chromosome 12 at 108,189,090 bp
  • C to T, chromosome 13 at 11,558,041 bp
  • A to T, chromosome 13 at 46,560,502 bp
  • G to T, chromosome 13 at 58,407,983 bp
  • A to G, chromosome 14 at 47,714,652 bp
  • A to T, chromosome 14 at 55,520,850 bp
  • T to A, chromosome 14 at 105,115,202 bp
  • T to C, chromosome 15 at 44,197,183 bp
  • T to A, chromosome 15 at 58,627,534 bp
  • C to T, chromosome 15 at 89,162,861 bp
  • T to G, chromosome 15 at 91,844,421 bp
  • T to C, chromosome 15 at 93,254,480 bp
  • A to T, chromosome 15 at 98,526,631 bp
  • A to G, chromosome 15 at 99,280,657 bp
  • C to T, chromosome 15 at 103,475,676 bp
  • C to T, chromosome 16 at 18,284,256 bp
  • C to T, chromosome 16 at 59,216,026 bp
  • C to G, chromosome 16 at 63,773,317 bp
  • G to A, chromosome 16 at 90,432,532 bp
  • A to T, chromosome 16 at 91,630,081 bp
  • A to G, chromosome 17 at 23,994,682 bp
  • A to G, chromosome 17 at 34,283,827 bp
  • A to T, chromosome 17 at 68,842,249 bp
  • A to T, chromosome 17 at 74,660,413 bp
  • A to T, chromosome 17 at 88,015,724 bp
  • C to T, chromosome 18 at 7,127,229 bp
  • T to C, chromosome 18 at 9,849,163 bp
  • A to T, chromosome 18 at 12,871,173 bp
  • A to G, chromosome 18 at 19,980,686 bp
  • A to C, chromosome 18 at 25,168,541 bp
  • A to G, chromosome 18 at 34,625,604 bp
  • A to G, chromosome 18 at 36,762,366 bp
  • A to G, chromosome 18 at 58,052,993 bp
  • G to T, chromosome 18 at 77,954,197 bp
  • G to A, chromosome 18 at 90,517,490 bp
  • G to A, chromosome 19 at 10,228,674 bp
  • A to G, chromosome 19 at 37,981,221 bp
  • A to G, chromosome X at 21,081,951 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2144 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040147-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.