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Strain Name:
C57BL/6J-MtgxR2168Btlr/Mmmh
Stock Number:
040171-MU
Citation ID:
RRID:MMRRC_040171-MU
Other Names:
R2168 (G1), C57BL/6J-MtgxR2168Btlr
Major Collection:

Strain Information

Sod1
Name: superoxide dismutase 1, soluble
Synonyms: CuZnSOD, SODC, Cu/Zn-SOD, Cu(2+)-Zn2+ superoxide dismutase, Sod-1, Ipo1, Ipo-1, B430204E11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20655
VEGA: 16
Homologene: 392
Neurog2
Name: neurogenin 2
Synonyms: Math4A, ngn2, Atoh4, bHLHa8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11924
Homologene: 7633
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: IMP-2, C330012H03Rik, IMP2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 319765
Homologene: 4774
Rfesd
Name: Rieske (Fe-S) domain containing
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218341
VEGA: 13
Homologene: 18282
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 162,710,334 bp
  • G to A, chromosome 1 at 167,078,650 bp
  • C to T, chromosome 1 at 178,015,911 bp
  • A to G, chromosome 1 at 191,983,527 bp
  • T to C, chromosome 2 at 14,244,204 bp
  • A to G, chromosome 2 at 30,104,875 bp
  • A to T, chromosome 2 at 41,375,846 bp
  • A to G, chromosome 2 at 88,910,178 bp
  • C to T, chromosome 2 at 91,628,348 bp
  • G to A, chromosome 2 at 101,886,331 bp
  • T to A, chromosome 2 at 127,878,781 bp
  • C to T, chromosome 3 at 93,201,937 bp
  • A to C, chromosome 3 at 100,187,883 bp
  • A to T, chromosome 3 at 127,634,105 bp
  • A to T, chromosome 3 at 153,867,835 bp
  • A to C, chromosome 4 at 65,156,445 bp
  • T to C, chromosome 4 at 70,433,442 bp
  • A to T, chromosome 4 at 112,086,986 bp
  • A to G, chromosome 4 at 139,410,649 bp
  • G to T, chromosome 4 at 145,087,323 bp
  • T to A, chromosome 4 at 156,221,882 bp
  • A to T, chromosome 4 at 156,338,378 bp
  • A to T, chromosome 5 at 21,575,048 bp
  • A to C, chromosome 5 at 142,506,963 bp
  • A to G, chromosome 6 at 113,591,159 bp
  • A to G, chromosome 6 at 121,870,499 bp
  • G to A, chromosome 6 at 128,488,047 bp
  • G to T, chromosome 6 at 146,111,678 bp
  • G to T, chromosome 7 at 19,730,614 bp
  • C to A, chromosome 7 at 79,915,267 bp
  • A to T, chromosome 7 at 102,942,471 bp
  • T to A, chromosome 9 at 55,743,639 bp
  • C to A, chromosome 9 at 110,888,742 bp
  • A to G, chromosome 10 at 89,414,098 bp
  • T to C, chromosome 11 at 60,187,596 bp
  • TCCCCCCCCCCC to TCCCCCCCCCCCCCCC, chromosome 11 at 119,321,798 bp
  • CCCC to CCCCCCCC, chromosome 11 at 119,321,806 bp
  • A to C, chromosome 11 at 119,415,070 bp
  • G to T, chromosome 12 at 4,633,044 bp
  • A to T, chromosome 12 at 17,373,584 bp
  • T to C, chromosome 12 at 104,149,369 bp
  • T to C, chromosome 13 at 4,081,106 bp
  • T to G, chromosome 13 at 21,537,252 bp
  • G to A, chromosome 13 at 23,114,544 bp
  • A to T, chromosome 13 at 33,666,374 bp
  • C to T, chromosome 13 at 60,800,935 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • T to C, chromosome 13 at 65,296,948 bp
  • T to A, chromosome 13 at 75,112,534 bp
  • T to C, chromosome 13 at 76,008,125 bp
  • T to C, chromosome 13 at 106,879,610 bp
  • T to A, chromosome 14 at 119,019,505 bp
  • C to A, chromosome 15 at 35,792,188 bp
  • C to A, chromosome 15 at 35,792,189 bp
  • A to T, chromosome 15 at 76,603,737 bp
  • T to A, chromosome 16 at 22,079,608 bp
  • C to A, chromosome 16 at 36,450,680 bp
  • T to A, chromosome 16 at 59,085,586 bp
  • T to C, chromosome 16 at 90,220,913 bp
  • T to C, chromosome 17 at 17,877,209 bp
  • T to A, chromosome 18 at 37,343,282 bp
  • A to G, chromosome 18 at 43,565,930 bp
  • A to T, chromosome 18 at 54,898,053 bp
  • G to A, chromosome 18 at 60,916,651 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2168 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040171-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.