Strain Name:
C57BL/6J-MtgxR2186Btlr/Mmmh
Stock Number:
040188-MU
Citation ID:
RRID:MMRRC_040188-MU
Other Names:
R2186 (G1), C57BL/6J-MtgxR2186Btlr
Major Collection:

Gene Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 269473
Homologene: 8882
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231051
Homologene: 46480
Lamb1
Name: laminin B1
Synonyms: Lamb-1, D130003D08Rik, Lamb1-1, C81607, C80098
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11287
Homologene: 104112
Fryl
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 2310004H21Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 72313
Homologene: 103956
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74996
Homologene: 9929
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: EG406236, Hsn2, 6430573H23Rik, Prkwnk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232341
Homologene: 14253
Ick
Name: intestinal cell kinase
Synonyms: 2210420N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56542
Homologene: 69218
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: D3Ertd775e, Lba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 77987
VEGA: 10
Homologene: 4973
Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: SF3b155, 2810001M05Rik, Prp10, Targ4, SAP155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 81898
Homologene: 6696
Exoc5
Name: exocyst complex component 5
Synonyms: SEC10, Sec10l1, PRO1912
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105504
Homologene: 38195
Zfp292
Name: zinc finger protein 292
Synonyms: 5730450D02Rik, Zfp15, 9430062L07Rik, Krox-10, Zfp-15, Zn-16, Zn-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 30046
Homologene: 8493
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Ccdc80
Name: coiled-coil domain containing 80
Synonyms: DRO1, 2610001E17Rik, Ssg1, Urb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 67896
Homologene: 12206
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: Solo, 6720464I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223435
VEGA: 15
Homologene: 20847
Mlh1
Name: mutL homolog 1
Synonyms: colon cancer, nonpolyposis type 2, 1110035C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17350
HGNC: HGNC:7127
Homologene: 208
Tlr4
Name: toll-like receptor 4
Synonyms: Rasl2-8, Lps, lipopolysaccharide response
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21898
Homologene: 41317
Fbxl18
Name: F-box and leucine-rich repeat protein 18
Synonyms: C330021B20Rik, B130019G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231863
Homologene: 41603
Med13
Name: mediator complex subunit 13
Synonyms: Trap240, 1110067M05Rik, Thrap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327987
Homologene: 21067
Rbm33
Name: RNA binding motif protein 33
Synonyms: 3200001K10Rik, 6430512A10Rik, Prr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381626
Homologene: 137386
Gpr183
Name: G protein-coupled receptor 183
Synonyms: Ebi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 321019
VEGA: 14
HGNC: HGNC:3128
Homologene: 28066
Ap1b1
Name: adaptor protein complex AP-1, beta 1 subunit
Synonyms: beta-prime adaptin, Adtb1, b2b1660Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11764
HGNC: HGNC:554
Homologene: 21972
Dnaja1
Name: DnaJ heat shock protein family (Hsp40) member A1
Synonyms: Hsj2, Nedd7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 15502
HGNC: HGNC:5229
Homologene: 55588
Cep164
Name: centrosomal protein 164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214552
Homologene: 51110
Vnn1
Name: vanin 1
Synonyms: V-1, pantetheinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22361
Homologene: 32130
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Cfap53
Name: cilia and flagella associated protein 53
Synonyms: Ccdc11, 4933415I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 74453
Homologene: 27056
Slc45a1
Name: solute carrier family 45, member 1
Synonyms: Dnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242773
Homologene: 44908
Dis3l
Name: DIS3 like exosome 3'-5' exoribonuclease
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 213550
Homologene: 15797
EU599041
Name: expressed sequence EU599041
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100170401
Cep85l
Name: centrosomal protein 85-like
Synonyms: Gm9766
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 100038725
VEGA: 10
Homologene: 52598
Cts8
Name: cathepsin 8
Synonyms: Epcs70, CTS2, Epcs68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56094
VEGA: 13
Homologene: 129897
Atp8b5
Name: ATPase, class I, type 8B, member 5
Synonyms: 4930417M19Rik, FetA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320571
Homologene: 99882
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330222
Homologene: 27395
Serpinb9d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9d
Synonyms: ovalbumin, AT2, Spi9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20726
HGNC: HGNC:8955
Homologene: 69093
Zfp286
Name: zinc finger protein 286
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192651
Homologene: 56892
Camk4
Name: calcium/calmodulin-dependent protein kinase IV
Synonyms: CaMKIV/Gr, D18Bwg0362e, Ca2+/calmodulin-dependent protein kinase type IV/Gr, A430110E23Rik, CaMKIV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12326
VEGA: 18
HGNC: HGNC:1464
Homologene: 100780
Atp8b4
Name: ATPase, class I, type 8B, member 4
Synonyms: Im
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241633
Homologene: 133162
Iqca1l
Name: IQ motif containing with AAA domain 1 like
Synonyms: 4931409K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231045
Homologene: 18602
Apbb1ip
Name: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Synonyms: 9930118P07Rik, Prp48, proline-rich protein 48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 54519
Homologene: 32434
Iqca
Name: IQ motif containing with AAA domain
Synonyms: 4930585L22Rik, 4930465P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74918
Homologene: 49784
Zbed6
Name: zinc finger, BED type containing 6
Synonyms: MGR, Gm8466, similar to Zinc finger BED domain containing protein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 667118
Homologene: 130066
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 4930589M24Rik, 3110012E06Rik, 4930438C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 75906
Homologene: 11600
Zfp28
Name: zinc finger protein 28
Synonyms: mkr-5, 2810438M17Rik, Zfp-28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22690
Homologene: 69047
Catsperb
Name: cation channel sperm associated auxiliary subunit beta
Synonyms: 4932415G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 271036
VEGA: 12
Homologene: 81904
B230325K18Rik
Name: RIKEN cDNA B230325K18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
Itpripl2
Name: inositol 1,4,5-triphosphate receptor interacting protein-like 2
Synonyms: E030018N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 319622
Homologene: 45882
Slc6a20b
Name: solute carrier family 6 (neurotransmitter transporter), member 20B
Synonyms: XT3, Xtrp3, Slc6a20, Sit1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22599
Homologene: 130652
Serpinb2
Name: serine (or cysteine) peptidase inhibitor, clade B, member 2
Synonyms: ovalbumin, PAI-2, Planh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18788
HGNC: HGNC:8584
Homologene: 20571
Asgr1
Name: asialoglycoprotein receptor 1
Synonyms: Asgr, Asgr-1, ASGPR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11889
HGNC: HGNC:742
Homologene: 1263
Tgfb2
Name: transforming growth factor, beta 2
Synonyms: Tgfb-2, Tgf-beta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 21808
Homologene: 2432
Mpp5
Name: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
Synonyms: Pals1, 3830420B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 56217
VEGA: 12
Homologene: 9512
Duoxa2
Name: dual oxidase maturation factor 2
Synonyms: 9030623N16Rik, Nip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66811
Homologene: 57037
2310033P09Rik
Name: RIKEN cDNA 2310033P09 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67862
Homologene: 134536
Habp4
Name: hyaluronic acid binding protein 4
Synonyms: 4933413D03Rik, 4933428J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56541
VEGA: 13
Homologene: 8615
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 55,007,633 bp
  • T to C, chromosome 1 at 90,081,344 bp
  • A to G, chromosome 1 at 107,523,964 bp
  • A to G, chromosome 1 at 133,658,079 bp
  • A to T, chromosome 1 at 186,704,418 bp
  • T to A, chromosome 2 at 22,835,904 bp
  • T to C, chromosome 2 at 122,299,174 bp
  • T to A, chromosome 2 at 126,358,860 bp
  • A to G, chromosome 3 at 86,304,336 bp
  • A to G, chromosome 3 at 104,468,598 bp
  • A to T, chromosome 4 at 34,807,962 bp
  • T to A, chromosome 4 at 40,732,853 bp
  • T to C, chromosome 4 at 43,366,583 bp
  • T to A, chromosome 4 at 66,839,983 bp
  • T to C, chromosome 4 at 150,638,251 bp
  • C to T, chromosome 5 at 24,554,526 bp
  • C to A, chromosome 5 at 25,287,112 bp
  • A to G, chromosome 5 at 28,394,230 bp
  • A to G, chromosome 5 at 73,064,975 bp
  • T to A, chromosome 5 at 142,046,292 bp
  • C to T, chromosome 5 at 142,878,761 bp
  • A to G, chromosome 6 at 119,948,567 bp
  • A to G, chromosome 6 at 128,498,946 bp
  • A to G, chromosome 7 at 6,394,498 bp
  • C to T, chromosome 7 at 43,225,909 bp
  • T to C, chromosome 7 at 112,087,983 bp
  • A to G, chromosome 7 at 118,491,277 bp
  • G to A, chromosome 7 at 127,985,534 bp
  • T to A, chromosome 9 at 45,768,578 bp
  • C to A, chromosome 9 at 64,339,612 bp
  • T to G, chromosome 9 at 66,439,901 bp
  • C to T, chromosome 9 at 78,131,487 bp
  • T to C, chromosome 9 at 111,258,566 bp
  • A to G, chromosome 9 at 123,603,901 bp
  • A to T, chromosome 10 at 23,897,401 bp
  • T to A, chromosome 10 at 50,718,278 bp
  • G to A, chromosome 10 at 53,348,618 bp
  • T to C, chromosome 10 at 53,638,194 bp
  • T to C, chromosome 11 at 5,015,737 bp
  • A to G, chromosome 11 at 59,208,514 bp
  • A to G, chromosome 11 at 62,780,461 bp
  • G to A, chromosome 11 at 70,056,249 bp
  • A to G, chromosome 11 at 86,288,292 bp
  • A to T, chromosome 12 at 31,318,467 bp
  • T to C, chromosome 12 at 78,819,371 bp
  • T to A, chromosome 12 at 101,480,782 bp
  • A to G, chromosome 13 at 33,203,047 bp
  • C to A, chromosome 13 at 61,251,731 bp
  • A to G, chromosome 13 at 64,182,266 bp
  • T to C, chromosome 14 at 49,015,479 bp
  • T to A, chromosome 14 at 121,954,315 bp
  • A to G, chromosome 15 at 27,823,975 bp
  • A to G, chromosome 16 at 45,118,105 bp
  • A to C, chromosome 18 at 33,182,341 bp
  • A to G, chromosome 18 at 44,812,078 bp
  • A to G, chromosome 18 at 74,329,505 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2186 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040188-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.