Strain Name:
C57BL/6J-MtgxR2194Btlr/Mmmh
Stock Number:
040196-MU
Citation ID:
RRID:MMRRC_040196-MU
Other Names:
R2194 (G1), C57BL/6J-MtgxR2194Btlr
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Sl, Mgf, Gb, grizzle-belly, Steel, SCF, blz, Kitlg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Hsp90aa1
Name: heat shock protein 90, alpha (cytosolic), class A member 1
Synonyms: hsp4, Hsp90, Hsp86-1, Hsp89, Hspca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 15519
HGNC: HGNC:5253
Homologene: 68464
Fkbp5
Name: FK506 binding protein 5
Synonyms: FKBP51, Dit1, D17Ertd592e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14229
HGNC: HGNC:3721
Homologene: 3038
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: SKD2, N-ethylmaleimide sensitive factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Hnrnpul1
Name: heterogeneous nuclear ribonucleoprotein U-like 1
Synonyms: E1B-AP5, E1BAP5, E130317O14Rik, Hnrnpul, Hnrpul1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232989
Homologene: 31419
Rnf40
Name: ring finger protein 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233900
Homologene: 8856
Hif1a
Name: hypoxia inducible factor 1, alpha subunit
Synonyms: MOP1, HIF-1alpha, HIF1alpha, bHLHe78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 15251
HGNC: HGNC:4910
Homologene: 1171
Slc25a40
Name: solute carrier family 25, member 40
Synonyms: B230315F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319653
Homologene: 6040
St7
Name: suppression of tumorigenicity 7
Synonyms: RAY1, HELG, SEN4, TSG7, Fam4a2, 9430001H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64213
Homologene: 10185
Dab1
Name: disabled 1
Synonyms: C630028C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13131
HGNC: HGNC:2661
Homologene: 32084
Gata2
Name: GATA binding protein 2
Synonyms: Gata-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14461
HGNC: HGNC:4171
Homologene: 32030
Ighmbp2
Name: immunoglobulin mu DNA binding protein 2
Synonyms: Catf1, AEP, sma, Smbp2, Smubp2, p110 subunit, RIPE3b1, Smbp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20589
HGNC: HGNC:5542
Homologene: 1642
Tnks1bp1
Name: tankyrase 1 binding protein 1
Synonyms: TAB182
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228140
Homologene: 14117
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola2, Cola-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Pcdhb4
Name: protocadherin beta 4
Synonyms: Pcdhb5A, PcdhbD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93875
HGNC: HGNC:8690
Homologene: 62176
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
RP24-170A20.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Zfp607a
Name: zinc finger protein 607A
Synonyms: 4732475C15Rik, Zfp607
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545938
Homologene: 134321
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268527
Homologene: 8780
Rfc4
Name: replication factor C (activator 1) 4
Synonyms: RFC37, A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106344
HGNC: HGNC:9972
Homologene: 6288
Cdh2
Name: cadherin 2
Synonyms: N-cadherin, Ncad, N-CAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Polb
Name: polymerase (DNA directed), beta
Synonyms: Pol beta, A430088C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18970
HGNC: HGNC:9174
Homologene: 2013
Or2t49
Name: olfactory receptor family 2 subfamily T member 49
Synonyms: GA_x6K02T2NKPP-912840-913784, MOR275-4, Olfr331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100502887
Homologene: 133015
Gm8104
Name: predicted gene 8104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 101056159
Rpl31-ps17
Name: ribosomal protein L31, pseudogene 17
Synonyms: Gm16382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 673582
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 85,063,065 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to T, chromosome 5 at 8,428,621 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • A to G, chromosome 6 at 17,942,719 bp
  • C to T, chromosome 6 at 88,204,574 bp
  • A to G, chromosome 7 at 25,725,922 bp
  • A to G, chromosome 7 at 27,879,380 bp
  • C to A, chromosome 7 at 127,597,235 bp
  • C to T, chromosome 8 at 22,647,467 bp
  • T to A, chromosome 10 at 39,892,923 bp
  • T to G, chromosome 10 at 100,016,037 bp
  • T to C, chromosome 11 at 58,501,642 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • T to C, chromosome 12 at 16,690,908 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • A to G, chromosome 12 at 73,930,747 bp
  • T to A, chromosome 12 at 110,695,680 bp
  • C to A, chromosome 12 at 110,695,681 bp
  • T to C, chromosome 14 at 43,101,560 bp
  • TTTGTTGTTGTTG to TTTGTTGTTG, chromosome 16 at 23,114,152 bp
  • T to C, chromosome 17 at 28,438,027 bp
  • T to C, chromosome 18 at 16,640,448 bp
  • T to C, chromosome 18 at 37,308,735 bp
  • T to C, chromosome 19 at 3,265,116 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2194 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040196-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.