Strain Name:
C57BL/6J-MtgxR2204Btlr/Mmmh
Stock Number:
040206-MU
Citation ID:
RRID:MMRRC_040206-MU
Other Names:
R2204 (G1), C57BL/6J-MtgxR2204Btlr
Major Collection:

Gene Information

Gene Symbol: Chrnb4 [MGI:87892] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Hlcs [MGI:894646] (Mus musculus (mouse))
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: D16Jhu34, 410I21.SP6
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Espl1 [MGI:2146156] (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, PRCE, separase, SSE, Cerp, ESP1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Fiz1 [MGI:1344336] (Mus musculus (mouse))
Name: Flt3 interacting zinc finger protein 1
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Prrc2b [MGI:1923304] (Mus musculus (mouse))
Name: proline-rich coiled-coil 2B
Synonyms: Bat2l, 5830434P21Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ift52 [MGI:2387217] (Mus musculus (mouse))
Name: intraflagellar transport 52
Synonyms: NGD5
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Plcb4 [MGI:107464] (Mus musculus (mouse))
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 75,430,477 bp
  • A to C, chromosome 1 at 87,767,015 bp
  • T to C, chromosome 1 at 172,532,161 bp
  • T to A, chromosome 1 at 173,270,136 bp
  • A to G, chromosome 1 at 191,065,164 bp
  • A to T, chromosome 2 at 5,855,983 bp
  • C to A, chromosome 2 at 32,223,464 bp
  • A to T, chromosome 2 at 37,352,044 bp
  • T to C, chromosome 2 at 76,771,641 bp
  • C to G, chromosome 2 at 88,746,609 bp
  • T to A, chromosome 2 at 118,830,975 bp
  • GAAA to GAA, chromosome 2 at 120,698,531 bp
  • C to A, chromosome 2 at 120,720,760 bp
  • T to A, chromosome 2 at 130,277,568 bp
  • A to G, chromosome 2 at 130,691,480 bp
  • T to C, chromosome 2 at 136,002,594 bp
  • G to A, chromosome 2 at 163,031,230 bp
  • T to A, chromosome 3 at 18,079,294 bp
  • A to G, chromosome 3 at 36,650,149 bp
  • A to G, chromosome 3 at 89,997,381 bp
  • T to A, chromosome 3 at 98,291,183 bp
  • T to C, chromosome 3 at 106,164,246 bp
  • A to G, chromosome 3 at 107,233,627 bp
  • C to T, chromosome 3 at 108,475,043 bp
  • G to T, chromosome 4 at 43,030,243 bp
  • G to A, chromosome 4 at 53,090,291 bp
  • A to G, chromosome 4 at 59,349,843 bp
  • A to T, chromosome 4 at 88,628,118 bp
  • T to C, chromosome 4 at 114,602,994 bp
  • A to C, chromosome 4 at 129,693,923 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to A, chromosome 5 at 146,493,729 bp
  • T to C, chromosome 6 at 8,558,488 bp
  • T to G, chromosome 6 at 18,408,694 bp
  • C to T, chromosome 6 at 29,459,508 bp
  • A to G, chromosome 6 at 30,641,819 bp
  • T to C, chromosome 6 at 57,926,619 bp
  • T to A, chromosome 6 at 118,523,882 bp
  • C to T, chromosome 7 at 5,008,686 bp
  • C to T, chromosome 7 at 17,975,348 bp
  • T to C, chromosome 7 at 28,749,674 bp
  • G to T, chromosome 8 at 45,023,700 bp
  • T to C, chromosome 8 at 48,674,550 bp
  • A to G, chromosome 8 at 83,570,899 bp
  • C to T, chromosome 8 at 122,813,644 bp
  • T to C, chromosome 9 at 20,055,211 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • G to A, chromosome 9 at 55,043,848 bp
  • A to G, chromosome 9 at 60,003,035 bp
  • T to A, chromosome 9 at 65,864,265 bp
  • A to T, chromosome 9 at 90,180,676 bp
  • T to C, chromosome 9 at 106,060,132 bp
  • A to G, chromosome 9 at 121,762,637 bp
  • A to G, chromosome 10 at 84,392,327 bp
  • G to A, chromosome 11 at 100,414,006 bp
  • T to A, chromosome 11 at 103,239,454 bp
  • C to T, chromosome 11 at 106,071,934 bp
  • T to C, chromosome 11 at 120,488,308 bp
  • A to G, chromosome 13 at 59,731,621 bp
  • A to G, chromosome 13 at 96,656,633 bp
  • A to T, chromosome 13 at 99,900,625 bp
  • T to C, chromosome 14 at 31,256,701 bp
  • A to G, chromosome 15 at 102,305,905 bp
  • T to A, chromosome 16 at 13,806,547 bp
  • A to G, chromosome 16 at 17,898,739 bp
  • T to C, chromosome 16 at 94,231,152 bp
  • T to C, chromosome 17 at 57,190,638 bp
  • A to G, chromosome 19 at 4,269,073 bp
  • A to T, chromosome 19 at 20,940,905 bp
  • C to T, chromosome 19 at 34,594,341 bp
  • C to A, chromosome 19 at 42,044,119 bp
  • C to A, chromosome 19 at 42,183,446 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2204 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040206-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.