Strain Name:
C57BL/6J-MtgxR2204Btlr/Mmmh
Stock Number:
040206-MU
Citation ID:
RRID:MMRRC_040206-MU
Other Names:
R2204 (G1), C57BL/6J-MtgxR2204Btlr
Major Collection:

Gene Information

Chrnb4
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108015
VEGA: 9
HGNC: HGNC:1964
Homologene: 20196
Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, separase, SSE, ESP1, PRCE, Cerp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Fiz1
Name: Flt3 interacting zinc finger protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23877
Homologene: 22663
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227723
Homologene: 106649
Ift52
Name: intraflagellar transport 52
Synonyms: NGD5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245866
Homologene: 9335
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Nudt5
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Glcci1
Name: glucocorticoid induced transcript 1
Synonyms: GIG18, 2310047L21Rik, Tssn1, A130036A18Rik, Fam117c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 170772
Homologene: 15773
Trip4
Name: thyroid hormone receptor interactor 4
Synonyms: ASC-1, 4930558E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56404
Homologene: 9426
Abca1
Name: ATP-binding cassette, sub-family A (ABC1), member 1
Synonyms: ABC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Nop56
Name: NOP56 ribonucleoprotein
Synonyms: NOP56, 56kDa with KKE/D repeat, 2310044F10Rik, Nol5a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67134
Homologene: 4660
Atg16l1
Name: autophagy related 16-like 1 (S. cerevisiae)
Synonyms: APG16L, WDR30, 1500009K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77040
Homologene: 41786
Pdcl
Name: phosducin-like
Synonyms: 1200011E13Rik, PhLP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67466
HGNC: HGNC:8770
Homologene: 38043
Rrn3
Name: RRN3 RNA polymerase I transcription factor homolog (yeast)
Synonyms: E130302O19Rik, TIF-1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106298
VEGA: 16
Homologene: 39531
Map3k14
Name: mitogen-activated protein kinase kinase kinase 14
Synonyms: Nik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53859
HGNC: HGNC:6853
Homologene: 2940
Cartpt
Name: CART prepropeptide
Synonyms: Cart
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27220
VEGA: 13
Homologene: 3169
Susd1
Name: sushi domain containing 1
Synonyms: Gm12528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 634731
Homologene: 11204
Elapor1
Name: endosome-lysosome associated apoptosis and autophagy regulator 1
Synonyms: Inceptor, Iir, 5330417C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229722
Homologene: 23249
Cdan1
Name: congenital dyserythropoietic anemia, type I (human)
Synonyms: CDA-I, CDA1, 1500015A01Rik, codanin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68968
HGNC: HGNC:1713
Homologene: 16324
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Knstrn
Name: kinetochore-localized astrin/SPAG5 binding
Synonyms: 1700025D04Rik, D2Ertd750e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51944
Homologene: 12198
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Ankrd26
Name: ankyrin repeat domain 26
Synonyms: 5730521P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232339
Homologene: 45968
Zfyve27
Name: zinc finger, FYVE domain containing 27
Synonyms: 9530077C24Rik, 2210011N02Rik, protrudin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 319740
Homologene: 16939
Nsl1
Name: NSL1, MIS12 kinetochore complex component
Synonyms: LOC381318, 4833432M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381318
Homologene: 22898
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Cyp7b1
Name: cytochrome P450, family 7, subfamily b, polypeptide 1
Synonyms: hct-1, D3Ertd552e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13123
HGNC: HGNC:2652
Homologene: 3544
Pramel32
Name: PRAME like 32
Synonyms: C87499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381590
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Tnfsf14
Name: tumor necrosis factor (ligand) superfamily, member 14
Synonyms: LIGHT, HVEM-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 50930
VEGA: 17
Homologene: 2822
Cpa1
Name: carboxypeptidase A1, pancreatic
Synonyms: 0910001L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 109697
HGNC: HGNC:2296
Homologene: 55609
Nuak1
Name: NUAK family, SNF1-like kinase, 1
Synonyms: B230104P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77976
Homologene: 8896
Stard9
Name: START domain containing 9
Synonyms: N-3 kinesin, Kif16a, 4831403C07Rik, E230025N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Slc4a11
Name: solute carrier family 4, sodium bicarbonate transporter-like, member 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269356
Homologene: 12931
Trpc3
Name: transient receptor potential cation channel, subfamily C, member 3
Synonyms: Trp3, Trrp3, Trcp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 22065
Homologene: 20708
Adamts7
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
Synonyms: ADAM-TS7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108153
HGNC: HGNC:223
Homologene: 22803
Mrpl12
Name: mitochondrial ribosomal protein L12
Synonyms: MRP-L12, Rpml12, 1500031N16Rik, 0610034O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56282
Homologene: 2212
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: EG235580, 1110001D15Rik, Dvwa, Vwa6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68553
Homologene: 130754
Flnc
Name: filamin C, gamma
Synonyms: Fln2, 1110055E19Rik, actin binding protein 280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Speg
Name: SPEG complex locus
Synonyms: BPEG, SPEGbeta, SPEGalpha, D1Bwg1450e, SPEG, Apeg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11790
Homologene: 55619
Ankrd2
Name: ankyrin repeat domain 2 (stretch responsive muscle)
Synonyms: mArpp, Arpp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56642
VEGA: 19
HGNC: HGNC:495
Homologene: 10568
Bap1
Name: Brca1 associated protein 1
Synonyms: 2300006C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 104416
HGNC: HGNC:950
Homologene: 3421
Chil3
Name: chitinase-like 3
Synonyms: Ym1, Chi3l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12655
Homologene: 74931
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, Cortbp2, 9130022E09Rik, 4732477G22Rik, 3010022N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 30785
Homologene: 14125
Zfp651
Name: zinc finger protein 651
Synonyms: 4732420M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270210
VEGA: 9
Homologene: 136302
Cfap45
Name: cilia and flagella associated protein 45
Synonyms: 1700028D05Rik, Nesg1, Ccdc19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71870
Homologene: 71837
Tssk2
Name: testis-specific serine kinase 2
Synonyms: Tsk2, SPOGA2, DGS-G, Stk22b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22115
VEGA: 16
Homologene: 31512
Acsf3
Name: acyl-CoA synthetase family member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257633
Homologene: 14958
Tmc1
Name: transmembrane channel-like gene family 1
Synonyms: Bth, Beethoven, 4933416G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13409
VEGA: 19
Homologene: 23670
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 207596
Homologene: 49796
Stoml2
Name: stomatin (Epb7.2)-like 2
Synonyms: 0610038F01Rik, SLP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66592
Homologene: 8389
Gm6370
Name: predicted gene 6370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622924
Homologene: 86127
Ssh3
Name: slingshot protein phosphatase 3
Synonyms: SSH-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 245857
VEGA: 19
Homologene: 32372
Vmn1r23
Name: vomeronasal 1 receptor 23
Synonyms: V1rc24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171197
Homologene: 110825
Ifit1bl1
Name: interferon induced protein with tetratricpeptide repeats 1B like 1
Synonyms: Gm14446
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 667373
Homologene: 78213
Tmem39b
Name: transmembrane protein 39b
Synonyms: 6330509E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230770
Homologene: 23069
Olfr418
Name: olfactory receptor 418
Synonyms: GA_x6K02T2P20D-20826777-20827719, GA_x6K02T2R7CC-581296-580364, MOR267-12P, MOR267-8, Olfr1403, Olfr418-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258645
HGNC: HGNC:8175
Homologene: 8218
Hmgcs2
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
Synonyms: mHS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15360
HGNC: HGNC:5008
Homologene: 38066
4932411G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Trabd2b
Name: TraB domain containing 2B
Synonyms: Gm12824, Hkat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666048
Homologene: 85034
Olfr1198
Name: olfactory receptor 1198
Synonyms: GA_x6K02T2Q125-50221692-50220766, MOR225-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 404330
Homologene: 138634
Hax1
Name: HCLS1 associated X-1
Synonyms: mHAX-1s, HAX-1, Silg111, Hs1bp1, HS1-associated protein X-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23897
Homologene: 4463
Olfr867
Name: olfactory receptor 867
Synonyms: GA_x6K02T2PVTD-13795933-13794938, MOR143-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 257898
Homologene: 128152
Sars2
Name: seryl-aminoacyl-tRNA synthetase 2
Synonyms: 2410015F05Rik, D7Ertd353e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71984
Homologene: 6073
Ceacam11
Name: carcinoembryonic antigen-related cell adhesion molecule 11
Synonyms: 1600015D01Rik, Ceacam11-C4, Ceacam11-C3, Ceacam11-C1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66996
Homologene: 135962
AC132405.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Ndufb7
Name: NADH:ubiquinone oxidoreductase subunit B7
Synonyms: 1110002H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66916
HGNC: HGNC:7702
Homologene: 3059
P3h4
Name: prolyl 3-hydroxylase family member 4 (non-enzymatic)
Synonyms: 1110036O03Rik, Leprel4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66180
Homologene: 4713
Taco1
Name: translational activator of mitochondrially encoded cytochrome c oxidase I
Synonyms: 2310066I18Rik, Ccdc44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70207
Homologene: 39079
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 75,430,477 bp
  • A to C, chromosome 1 at 87,767,015 bp
  • T to C, chromosome 1 at 172,532,161 bp
  • T to A, chromosome 1 at 173,270,136 bp
  • A to G, chromosome 1 at 191,065,164 bp
  • A to T, chromosome 2 at 5,855,983 bp
  • C to A, chromosome 2 at 32,223,464 bp
  • A to T, chromosome 2 at 37,352,044 bp
  • T to C, chromosome 2 at 76,771,641 bp
  • C to G, chromosome 2 at 88,746,609 bp
  • T to A, chromosome 2 at 118,830,975 bp
  • GAAA to GAA, chromosome 2 at 120,698,531 bp
  • C to A, chromosome 2 at 120,720,760 bp
  • T to A, chromosome 2 at 130,277,568 bp
  • A to G, chromosome 2 at 130,691,480 bp
  • T to C, chromosome 2 at 136,002,594 bp
  • G to A, chromosome 2 at 163,031,230 bp
  • T to A, chromosome 3 at 18,079,294 bp
  • A to G, chromosome 3 at 36,650,149 bp
  • A to G, chromosome 3 at 89,997,381 bp
  • T to A, chromosome 3 at 98,291,183 bp
  • T to C, chromosome 3 at 106,164,246 bp
  • A to G, chromosome 3 at 107,233,627 bp
  • C to T, chromosome 3 at 108,475,043 bp
  • G to T, chromosome 4 at 43,030,243 bp
  • G to A, chromosome 4 at 53,090,291 bp
  • A to G, chromosome 4 at 59,349,843 bp
  • A to T, chromosome 4 at 88,628,118 bp
  • T to C, chromosome 4 at 114,602,994 bp
  • A to C, chromosome 4 at 129,693,923 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to A, chromosome 5 at 146,493,729 bp
  • T to C, chromosome 6 at 8,558,488 bp
  • T to G, chromosome 6 at 18,408,694 bp
  • C to T, chromosome 6 at 29,459,508 bp
  • A to G, chromosome 6 at 30,641,819 bp
  • T to C, chromosome 6 at 57,926,619 bp
  • T to A, chromosome 6 at 118,523,882 bp
  • C to T, chromosome 7 at 5,008,686 bp
  • C to T, chromosome 7 at 17,975,348 bp
  • T to C, chromosome 7 at 28,749,674 bp
  • G to T, chromosome 8 at 45,023,700 bp
  • T to C, chromosome 8 at 48,674,550 bp
  • A to G, chromosome 8 at 83,570,899 bp
  • C to T, chromosome 8 at 122,813,644 bp
  • T to C, chromosome 9 at 20,055,211 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • G to A, chromosome 9 at 55,043,848 bp
  • A to G, chromosome 9 at 60,003,035 bp
  • T to A, chromosome 9 at 65,864,265 bp
  • A to T, chromosome 9 at 90,180,676 bp
  • T to C, chromosome 9 at 106,060,132 bp
  • A to G, chromosome 9 at 121,762,637 bp
  • A to G, chromosome 10 at 84,392,327 bp
  • G to A, chromosome 11 at 100,414,006 bp
  • T to A, chromosome 11 at 103,239,454 bp
  • C to T, chromosome 11 at 106,071,934 bp
  • T to C, chromosome 11 at 120,488,308 bp
  • A to G, chromosome 13 at 59,731,621 bp
  • A to G, chromosome 13 at 96,656,633 bp
  • A to T, chromosome 13 at 99,900,625 bp
  • T to C, chromosome 14 at 31,256,701 bp
  • A to G, chromosome 15 at 102,305,905 bp
  • T to A, chromosome 16 at 13,806,547 bp
  • A to G, chromosome 16 at 17,898,739 bp
  • T to C, chromosome 16 at 94,231,152 bp
  • T to C, chromosome 17 at 57,190,638 bp
  • A to G, chromosome 19 at 4,269,073 bp
  • A to T, chromosome 19 at 20,940,905 bp
  • C to T, chromosome 19 at 34,594,341 bp
  • C to A, chromosome 19 at 42,044,119 bp
  • C to A, chromosome 19 at 42,183,446 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2204 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040206-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.