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Strain Name:
C57BL/6J-MtgxR2205Btlr/Mmmh
Stock Number:
040207-MU
Citation ID:
RRID:MMRRC_040207-MU
Other Names:
R2205 (G1), C57BL/6J-MtgxR2205Btlr
Major Collection:

Strain Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Ywhah
Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
Synonyms: 14-3-3 eta
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22629
Homologene: 37767
Nudt5
Name: nudix hydrolase 5
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 119,936,104 bp
  • T to C, chromosome 1 at 172,532,161 bp
  • A to T, chromosome 2 at 5,855,983 bp
  • C to T, chromosome 2 at 66,235,123 bp
  • T to C, chromosome 2 at 76,851,563 bp
  • T to A, chromosome 2 at 153,261,247 bp
  • A to G, chromosome 2 at 155,851,485 bp
  • G to A, chromosome 2 at 163,472,596 bp
  • T to A, chromosome 3 at 98,291,183 bp
  • T to C, chromosome 3 at 106,164,246 bp
  • C to T, chromosome 3 at 108,475,043 bp
  • A to G, chromosome 3 at 108,757,293 bp
  • T to A, chromosome 3 at 123,556,833 bp
  • A to T, chromosome 4 at 11,225,712 bp
  • G to T, chromosome 4 at 43,030,243 bp
  • A to C, chromosome 4 at 129,693,923 bp
  • A to G, chromosome 4 at 133,065,909 bp
  • A to G, chromosome 5 at 33,027,140 bp
  • A to T, chromosome 5 at 74,225,367 bp
  • A to G, chromosome 5 at 90,569,562 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • G to A, chromosome 5 at 114,389,074 bp
  • A to G, chromosome 5 at 121,818,691 bp
  • T to A, chromosome 5 at 122,681,101 bp
  • G to A, chromosome 5 at 140,705,133 bp
  • T to A, chromosome 5 at 146,493,729 bp
  • T to C, chromosome 6 at 8,558,488 bp
  • T to G, chromosome 6 at 18,408,694 bp
  • C to T, chromosome 6 at 29,459,508 bp
  • A to G, chromosome 6 at 30,641,819 bp
  • T to C, chromosome 6 at 57,926,619 bp
  • T to A, chromosome 6 at 118,523,882 bp
  • A to T, chromosome 7 at 16,498,025 bp
  • C to T, chromosome 7 at 25,341,748 bp
  • T to A, chromosome 7 at 29,154,410 bp
  • G to A, chromosome 7 at 29,185,246 bp
  • T to A, chromosome 7 at 30,258,149 bp
  • C to A, chromosome 7 at 35,291,772 bp
  • T to C, chromosome 7 at 46,002,567 bp
  • T to C, chromosome 7 at 120,247,280 bp
  • G to T, chromosome 8 at 84,225,869 bp
  • A to G, chromosome 9 at 60,003,035 bp
  • A to T, chromosome 9 at 90,180,676 bp
  • T to C, chromosome 9 at 106,717,798 bp
  • C to A, chromosome 9 at 109,082,226 bp
  • A to G, chromosome 9 at 121,762,637 bp
  • C to A, chromosome 11 at 4,889,974 bp
  • T to C, chromosome 11 at 8,531,719 bp
  • A to G, chromosome 11 at 23,385,147 bp
  • A to G, chromosome 11 at 46,528,567 bp
  • C to G, chromosome 11 at 49,019,322 bp
  • G to A, chromosome 11 at 54,324,007 bp
  • A to G, chromosome 11 at 76,802,244 bp
  • T to C, chromosome 11 at 94,363,685 bp
  • T to A, chromosome 11 at 96,345,668 bp
  • C to A, chromosome 11 at 101,634,803 bp
  • A to T, chromosome 12 at 72,656,370 bp
  • A to G, chromosome 12 at 111,317,902 bp
  • T to C, chromosome 13 at 112,898,890 bp
  • C to T, chromosome 14 at 70,164,779 bp
  • A to G, chromosome 15 at 57,953,520 bp
  • G to A, chromosome 15 at 84,202,314 bp
  • A to T, chromosome 17 at 20,029,821 bp
  • C to T, chromosome 17 at 25,380,260 bp
  • A to T, chromosome 17 at 26,092,140 bp
  • G to A, chromosome 17 at 35,144,607 bp
  • A to C, chromosome 17 at 56,199,287 bp
  • T to C, chromosome 17 at 57,190,638 bp
  • A to G, chromosome 19 at 4,269,073 bp
  • A to T, chromosome 19 at 20,940,905 bp
  • C to T, chromosome 19 at 34,594,341 bp
  • C to A, chromosome 19 at 42,044,119 bp
  • C to A, chromosome 19 at 42,183,446 bp
  • T to A, chromosome X at 52,397,206 bp
  • G to A, chromosome X at 74,017,138 bp
  • A to G, chromosome X at 134,973,112 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2205 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040207-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.