Strain Name:
C57BL/6J-MtgxR2209Btlr/Mmmh
Stock Number:
040211-MU
Citation ID:
RRID:MMRRC_040211-MU
Other Names:
R2209 (G1), C57BL/6J-MtgxR2209Btlr
Major Collection:

Gene Information

Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Zbtb25
Name: zinc finger and BTB domain containing 25
Synonyms: Zfp50, 2900064P18Rik, 2810462M08Rik, Kup, Zfp-50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109929
Homologene: 5070
Rps19
Name: ribosomal protein S19
Synonyms: Dsk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20085
Homologene: 37416
Lrrc41
Name: leucine rich repeat containing 41
Synonyms: D730026A16Rik, D630045E04Rik, MUF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230654
Homologene: 4645
Nkx2-1
Name: NK2 homeobox 1
Synonyms: tinman, Ttf-1, thyroid transcription factor-1, Titf1, thyroid-specific enhancer-binding protein, T/EBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21869
Homologene: 2488
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: Ae2, B3RP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20535
Homologene: 128699
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Brpf3
Name: bromodomain and PHD finger containing, 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268936
Homologene: 16092
Specc1l
Name: sperm antigen with calponin homology and coiled-coil domains 1-like
Synonyms: 4930470P14Rik, 9530057A13Rik, Specc1l, Cytsa, 4932439K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74392
VEGA: 10
Homologene: 15610
Smc3
Name: structural maintenance of chromosomes 3
Synonyms: Cspg6, Mmip1, Bamacan, SmcD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13006
HGNC: HGNC:2468
Homologene: 3974
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: D3Ertd775e, Lba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Flnb
Name: filamin, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: E230024D05Rik, 9030416H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71521
Homologene: 22877
Cry1
Name: cryptochrome 1 (photolyase-like)
Synonyms: Phll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12952
VEGA: 10
HGNC: HGNC:2384
Homologene: 7042
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: Tgfb1i4, TSC-22, Egr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21807
Homologene: 7573
Lman2
Name: lectin, mannose-binding 2
Synonyms: VIP36, 1300009F09Rik, 1110003H06Rik, GP36B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66890
VEGA: 13
Homologene: 4962
Ncor2
Name: nuclear receptor co-repressor 2
Synonyms: SMRTe, SMRT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20602
HGNC: HGNC:7673
Homologene: 31370
Cenpf
Name: centromere protein F
Synonyms: mitosin, Lek1, 6530404A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Dcp2
Name: decapping mRNA 2
Synonyms: 2410015D23Rik, 5730537H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70640
Homologene: 13968
Sec23b
Name: SEC23 homolog B, COPII coat complex component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27054
Homologene: 74571
Naf1
Name: nuclear assembly factor 1 ribonucleoprotein
Synonyms: LOC234344
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234344
Homologene: 128518
Ticam2
Name: toll-like receptor adaptor molecule 2
Synonyms: TRAM, Tirp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225471
Homologene: 11014
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Igfbp5
Name: insulin-like growth factor binding protein 5
Synonyms: IGFBP-5, IGFBP-5P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16011
HGNC: HGNC:5474
Homologene: 56489
Prpf39
Name: pre-mRNA processing factor 39
Synonyms: Srcs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 328110
Homologene: 32377
Ripor2
Name: RHO family interacting cell polarization regulator 2
Synonyms: 1700108N18Rik, 6330500D04Rik, Fam65b, E430013J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 193385
Homologene: 9284
Notch1
Name: notch 1
Synonyms: 9930111A19Rik, N1, Motch A, Mis6, lin-12, Tan1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18128
HGNC: HGNC:7881
Homologene: 32049
Src
Name: Rous sarcoma oncogene
Synonyms: pp60c-src
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20779
Homologene: 21120
Mtx3
Name: metaxin 3
Synonyms: 4930470O13Rik, LOC382793, Gm6514
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 382793
VEGA: 13
Homologene: 46611
Thsd1
Name: thrombospondin, type I, domain 1
Synonyms: 4833423O18Rik, Tmtsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56229
Homologene: 10264
Igflr1
Name: IGF-like family receptor 1
Synonyms: F630003I17Rik, Tmem149
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101883
Homologene: 49779
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: SkM1, Nav1.4, mH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 110880
Homologene: 283
Nutm2
Name: NUT family member 2
Synonyms: LOC328250, Gm806
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328250
Homologene: 128615
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: C130041O22Rik, Zfh-4, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80892
Homologene: 23477
Zmiz2
Name: zinc finger, MIZ-type containing 2
Synonyms: D11Bwg0280e, Zimp7, 2410117E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52915
Homologene: 18000
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Prl8a6
Name: prolactin family 8, subfamily a, member 6
Synonyms: Prlpc, Prlpc1, PLP-Ca, PLP-calpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19112
Pde10a
Name: phosphodiesterase 10A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 23984
HGNC: HGNC:8772
Homologene: 4852
Gm4884
Name: predicted gene 4884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233164
Nudt8
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 8
Synonyms: 2310039H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66387
VEGA: 19
HGNC: HGNC:8055
Homologene: 41633
Il1r2
Name: interleukin 1 receptor, type II
Synonyms: CD121b, Il1r-2, IL-1 receptor beta chain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16178
HGNC: HGNC:5994
Homologene: 7783
Emid1
Name: EMI domain containing 1
Synonyms: CO-5, Emu1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 140703
Homologene: 135639
Prkcg
Name: protein kinase C, gamma
Synonyms: Prkcc, PKCgamma, Pkcc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18752
HGNC: HGNC:9402
Homologene: 20602
Rmnd5a
Name: required for meiotic nuclear division 5 homolog A
Synonyms: Gid2, 1110007A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68477
Homologene: 5668
Ecm2
Name: extracellular matrix protein 2, female organ and adipocyte specific
Synonyms: 9030618O22Rik, tenonectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 407800
VEGA: 13
HGNC: HGNC:3154
Homologene: 1064
Stxbp5l
Name: syntaxin binding protein 5-like
Synonyms: insulin level locus 1, A830015P08Rik, t2md1, LLGL4, T2dm1, tomosyn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207227
Homologene: 18173
Lrrc6
Name: leucine rich repeat containing 6 (testis)
Synonyms: LRTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54562
Homologene: 34534
Gm14412
Name: predicted gene 14412
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 102640477
Homologene: 134324
Dhx30
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 30
Synonyms: C130058C04Rik, helG, 2810477H02Rik, Ddx30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72831
Homologene: 15779
Flnc
Name: filamin C, gamma
Synonyms: Fln2, 1110055E19Rik, actin binding protein 280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Fes
Name: feline sarcoma oncogene
Synonyms: c-fes
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14159
HGNC: HGNC:3657
Homologene: 37563
Vmn1r63
Name: vomeronasal 1 receptor 63
Synonyms: V1R1, V1rd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81017
Homologene: 41799
6430548M08Rik
Name: RIKEN cDNA 6430548M08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234797
Homologene: 8825
Krt87
Name: keratin 87
Synonyms: Krt83, Krt2-25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406219
VEGA: 15
Homologene: 133804
Arsb
Name: arylsulfatase B
Synonyms: As-1s, As-1t, Asr-1, Ast-1, 1110007C02Rik, As-1, As-1r, As1-s, As1, As1-r, As1-t
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11881
VEGA: 13
HGNC: HGNC:714
Homologene: 73870
Mark1
Name: MAP/microtubule affinity regulating kinase 1
Synonyms: B930025N23Rik, Emk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226778
HGNC: HGNC:6896
Homologene: 49552
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: 3230402H02Rik, VE-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Phyhip
Name: phytanoyl-CoA hydroxylase interacting protein
Synonyms: PAHX-AP1, PAHX-AP#1, C630010D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105653
Homologene: 8847
Ccdc130
Name: coiled-coil domain containing 130
Synonyms: 4930527D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67736
Homologene: 12183
Tspoap1
Name: TSPO associated protein 1
Synonyms: Bzrap1, peripheral
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207777
Homologene: 37961
Rusc1
Name: RUN and SH3 domain containing 1
Synonyms: 2210403N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72296
Homologene: 75028
Tspan10
Name: tetraspanin 10
Synonyms: Ocsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 208634
Homologene: 49972
Olfr1434
Name: olfactory receptor 1434
Synonyms: GA_x6K02T2RE5P-2610001-2610414, Olfr1433, GA_x6K02T2RE5P-2618516-2619454, MOR214-4, MOR214-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258680
Homologene: 45029
Cyp21a1
Name: cytochrome P450, family 21, subfamily a, polypeptide 1
Synonyms: 21-hydroxylase, Cyp21, 21-OH, 21OH, 21OHA, Oh21-1, Oh21-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13079
Homologene: 68063
Mrpl38
Name: mitochondrial ribosomal protein L38
Synonyms: 1110036N21Rik, MRP-L3, Rpml3, 4733401F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 60441
Homologene: 11476
Zfp84
Name: zinc finger protein 84
Synonyms: 2210410P13Rik, C86188, 4633401C23Rik, Zfp69, KRAB18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74352
Homologene: 51858
Exoc8
Name: exocyst complex component 8
Synonyms: Exo84p, SEC84, EXO84
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102058
Homologene: 44545
Pet112l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Slc7a12
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 12
Synonyms: XAT1, Asc-2, asc-type amino acid transporter 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140918
Homologene: 76812
Sst
Name: somatostatin
Synonyms: preprosomatostatin, Smst, SRIF, SOM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20604
VEGA: 16
Homologene: 819
Ttc33
Name: tetratricopeptide repeat domain 33
Synonyms: 2900001O04Rik, 2410099M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67515
Homologene: 8226
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,115,138 bp
  • A to G, chromosome 1 at 72,873,937 bp
  • A to T, chromosome 1 at 184,919,504 bp
  • A to T, chromosome 1 at 189,652,598 bp
  • T to A, chromosome 2 at 20,849,520 bp
  • C to A, chromosome 2 at 26,460,007 bp
  • A to G, chromosome 2 at 32,250,862 bp
  • T to G, chromosome 2 at 144,564,662 bp
  • A to G, chromosome 2 at 157,462,790 bp
  • A to G, chromosome 2 at 177,317,436 bp
  • T to A, chromosome 3 at 5,396,918 bp
  • A to G, chromosome 3 at 14,481,064 bp
  • G to A, chromosome 3 at 85,653,805 bp
  • A to G, chromosome 3 at 86,225,519 bp
  • A to G, chromosome 3 at 89,088,821 bp
  • T to A, chromosome 4 at 116,095,780 bp
  • G to T, chromosome 5 at 24,427,646 bp
  • A to T, chromosome 5 at 65,628,014 bp
  • A to G, chromosome 5 at 125,124,757 bp
  • T to A, chromosome 6 at 29,455,845 bp
  • C to T, chromosome 6 at 71,439,983 bp
  • A to C, chromosome 7 at 3,303,581 bp
  • T to A, chromosome 7 at 5,803,213 bp
  • C to T, chromosome 7 at 24,885,127 bp
  • T to A, chromosome 7 at 29,777,182 bp
  • T to A, chromosome 7 at 30,567,797 bp
  • T to C, chromosome 7 at 41,043,321 bp
  • A to T, chromosome 7 at 80,380,283 bp
  • T to A, chromosome 8 at 22,258,871 bp
  • T to G, chromosome 8 at 66,860,536 bp
  • C to T, chromosome 8 at 84,263,869 bp
  • A to G, chromosome 8 at 120,157,488 bp
  • C to T, chromosome 8 at 124,896,179 bp
  • T to C, chromosome 9 at 79,692,352 bp
  • C to T, chromosome 9 at 110,085,142 bp
  • A to G, chromosome 10 at 75,246,576 bp
  • A to G, chromosome 10 at 85,146,755 bp
  • A to G, chromosome 10 at 116,369,357 bp
  • G to A, chromosome 11 at 5,135,407 bp
  • A to G, chromosome 11 at 6,400,889 bp
  • C to T, chromosome 11 at 87,765,891 bp
  • T to A, chromosome 11 at 106,339,225 bp
  • T to C, chromosome 11 at 116,138,462 bp
  • T to C, chromosome 11 at 120,446,163 bp
  • A to C, chromosome 12 at 8,007,752 bp
  • T to G, chromosome 12 at 56,533,508 bp
  • T to C, chromosome 12 at 65,057,915 bp
  • A to G, chromosome 12 at 76,349,129 bp
  • A to T, chromosome 13 at 24,701,612 bp
  • C to T, chromosome 13 at 27,435,386 bp
  • A to G, chromosome 13 at 49,530,156 bp
  • A to G, chromosome 13 at 50,473,135 bp
  • A to G, chromosome 13 at 55,351,502 bp
  • A to G, chromosome 13 at 92,847,604 bp
  • A to G, chromosome 13 at 93,862,101 bp
  • G to T, chromosome 14 at 7,905,507 bp
  • A to T, chromosome 14 at 70,461,894 bp
  • C to A, chromosome 14 at 76,418,740 bp
  • A to G, chromosome 15 at 5,208,443 bp
  • A to G, chromosome 15 at 66,449,551 bp
  • T to C, chromosome 15 at 101,433,108 bp
  • T to C, chromosome 16 at 23,889,808 bp
  • T to A, chromosome 16 at 37,216,036 bp
  • T to C, chromosome 17 at 8,944,930 bp
  • G to A, chromosome 17 at 28,828,420 bp
  • C to A, chromosome 17 at 34,802,727 bp
  • A to T, chromosome 18 at 44,405,514 bp
  • A to T, chromosome 18 at 46,560,400 bp
  • T to C, chromosome 19 at 4,001,902 bp
  • A to G, chromosome 19 at 6,493,007 bp
  • T to G, chromosome 19 at 12,283,860 bp
  • T to A, chromosome 19 at 53,618,760 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2209 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040211-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.