Strain Name:
C57BL/6J-MtgxR2225Btlr/Mmmh
Stock Number:
040226-MU
Citation ID:
RRID:MMRRC_040226-MU
Other Names:
R2225 (G1), C57BL/6J-MtgxR2225Btlr
Major Collection:

Strain Information

Aggf1
Name: angiogenic factor with G patch and FHA domains 1
Synonyms: 2310029P06Rik, VG5Q, 2010009L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66549
Homologene: 41220
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268281
Homologene: 6489
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 271639
Homologene: 10188
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224273
Homologene: 28544
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: P140SRA-1, Shyc, pl-1, l(7)1Rl, Sra-1, E030028J09Rik, l7Rl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20430
Homologene: 22628
Hspa4
Name: heat shock protein 4
Synonyms: APG-2, Hsp70RY, Hsp110, 70kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15525
HGNC: HGNC:5237
Homologene: 1624
Sf3b3
Name: splicing factor 3b, subunit 3
Synonyms: 5730409A01Rik, 1810061H24Rik, SAP130, RSE1, D8Ertd633e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101943
Homologene: 6579
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Kpna1
Name: karyopherin (importin) alpha 1
Synonyms: mSRP1, m-importin-alpha-S1, Rch2, NPI1, importin alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16646
HGNC: HGNC:6394
Homologene: 55642
Cpsf6
Name: cleavage and polyadenylation specific factor 6
Synonyms: HPBRII-7, HPBRII-4, CFIM68, 4733401N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432508
Homologene: 134126
Sfxn1
Name: sideroflexin 1
Synonyms: 2810002O05Rik, A930015P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Aen
Name: apoptosis enhancing nuclease
Synonyms: 2700083B06Rik, Isg20l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68048
Homologene: 23382
Gins4
Name: GINS complex subunit 4 (Sld5 homolog)
Synonyms: 4933405K01Rik, SLD5, 2810037C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109145
Homologene: 5759
Limk1
Name: LIM-domain containing, protein kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16885
HGNC: HGNC:6613
Homologene: 1738
Itgb3
Name: integrin beta 3
Synonyms: platelet glycoprotein IIIa (GP3A), CD61
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16416
HGNC: HGNC:6156
Homologene: 55444
Zfp738
Name: zinc finger protein 738
Synonyms: 6720487G11Rik, 3830402I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408068
Homologene: 133713
Zfp451
Name: zinc finger protein 451
Synonyms: Kiaa0576-hp, 4933435G09Rik, 4930515K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98403
Homologene: 9188
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53614
Homologene: 9622
Srsf6
Name: serine and arginine-rich splicing factor 6
Synonyms: 1210001E11Rik, Sfrs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67996
Homologene: 110783
Cwc22
Name: CWC22 spliceosome-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 80744
Homologene: 6048
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Eif5b
Name: eukaryotic translation initiation factor 5B
Synonyms: IF2, A030003E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226982
Homologene: 134613
Lonrf1
Name: LON peptidase N-terminal domain and ring finger 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244421
Homologene: 34980
Rundc1
Name: RUN domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217201
Homologene: 15095
Rpgrip1l
Name: Rpgrip1-like
Synonyms: Ftm, 1700047E16Rik, fantom, Nphp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244585
Homologene: 18296
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Nkpd1
Name: NTPase, KAP family P-loop domain containing 1
Synonyms: 2310015G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69547
Homologene: 18876
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329278
Homologene: 18634
Rnf185
Name: ring finger protein 185
Synonyms: 1700022N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193670
Homologene: 34298
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f1, Myhs-f, Myhsf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Htra4
Name: HtrA serine peptidase 4
Synonyms: B430206E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330723
Homologene: 17801
Or8g33
Name: olfactory receptor family 8 subfamily G member 33
Synonyms: GA_x6K02T2PVTD-33124064-33123120, MOR171-21, Olfr952
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235248
VEGA: 9
Homologene: 133698
Or10d5
Name: olfactory receptor family 10 subfamily D member 5
Synonyms: GA_x6K02T2PVTD-33651220-33650288, MOR224-2, Olfr975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258825
VEGA: 9
Homologene: 27275
Itih1
Name: inter-alpha trypsin inhibitor, heavy chain 1
Synonyms: inter-alpha (globulin) inhibitor, H1 polypeptide, Intin1, Itih-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16424
HGNC: HGNC:6166
Homologene: 1667
Naalad2
Name: N-acetylated alpha-linked acidic dipeptidase 2
Synonyms: NAALADASE2, NAADALASE2, D9Ertd285e, GCPIII, GCP3, Folh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72560
Homologene: 3294
Tmem270
Name: transmembrane protein 270
Synonyms: 1700111I05Rik, Wbscr28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76629
Homologene: 18790
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330222
Homologene: 27395
Eri2
Name: exoribonuclease 2
Synonyms: 4933424N09Rik, Exod1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71151
Homologene: 12383
Tph1
Name: tryptophan hydroxylase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21990
Homologene: 121565
Wiz
Name: widely-interspaced zinc finger motifs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22404
Homologene: 7997
Tbxa2r
Name: thromboxane A2 receptor
Synonyms: TP, Tp receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21390
VEGA: 10
Homologene: 825
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: LOC382077, 4930535E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Fam75d3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Hdac7b, Mitr, D030072B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 79221
Homologene: 128578
Abtb1
Name: ankyrin repeat and BTB (POZ) domain containing 1
Synonyms: BPOZ, EF1ABP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 80283
Homologene: 32731
Or2l5
Name: olfactory receptor family 2 subfamily L member 5
Synonyms: GA_x54KRFPKG5P-15963726-15962788, MOR272-1, Olfr167
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258937
Homologene: 78689
Tlr5
Name: toll-like receptor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 53791
Homologene: 20698
Tnnt2
Name: troponin T2, cardiac
Synonyms: Tnt, cTnT, cardiac TnT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21956
Homologene: 68050
Dld
Name: dihydrolipoamide dehydrogenase
Synonyms: dihydrolipoyl dehydrogenase, branched chain alpha-keto acid dehydrogenase complex subunit E3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13382
VEGA: 12
HGNC: HGNC:2898
Homologene: 84
Plekhg2
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 2
Synonyms: Clg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101497
Homologene: 16341
Ddah2
Name: dimethylarginine dimethylaminohydrolase 2
Synonyms: Clone 7u, Ddah, NG30, G6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 51793
HGNC: HGNC:2716
Homologene: 8510
Tmtc2
Name: transmembrane and tetratricopeptide repeat containing 2
Synonyms: D330034A10Rik, 8430438D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 278279
Homologene: 25521
Btd
Name: biotinidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 26363
HGNC: HGNC:1122
Homologene: 37255
Hoxa10
Name: homeobox A10
Synonyms: Hoxa-10, Hox-1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15395
HGNC: HGNC:5100
Homologene: 7365
Mvb12b
Name: multivesicular body subunit 12B
Synonyms: 2610200O14Rik, 2610528K11Rik, Fam125b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72543
Homologene: 25444
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 33,770,907 bp
  • A to T, chromosome 1 at 38,019,223 bp
  • A to G, chromosome 1 at 135,843,791 bp
  • A to T, chromosome 1 at 160,147,465 bp
  • A to G, chromosome 1 at 165,518,260 bp
  • A to G, chromosome 1 at 182,972,376 bp
  • G to A, chromosome 2 at 33,840,199 bp
  • T to A, chromosome 2 at 77,908,151 bp
  • T to C, chromosome 2 at 125,581,784 bp
  • G to A, chromosome 2 at 162,931,699 bp
  • A to G, chromosome 4 at 43,922,837 bp
  • A to T, chromosome 5 at 4,077,271 bp
  • G to T, chromosome 5 at 134,661,556 bp
  • A to G, chromosome 5 at 134,906,638 bp
  • T to C, chromosome 5 at 142,158,655 bp
  • T to C, chromosome 6 at 52,232,636 bp
  • A to C, chromosome 6 at 88,836,367 bp
  • A to T, chromosome 7 at 19,519,820 bp
  • G to A, chromosome 7 at 28,360,335 bp
  • A to G, chromosome 7 at 46,665,174 bp
  • G to A, chromosome 7 at 55,926,275 bp
  • C to A, chromosome 7 at 78,902,451 bp
  • A to G, chromosome 7 at 119,772,622 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • T to C, chromosome 8 at 23,236,966 bp
  • T to C, chromosome 8 at 25,025,720 bp
  • T to C, chromosome 8 at 36,236,098 bp
  • T to C, chromosome 8 at 91,221,467 bp
  • T to C, chromosome 8 at 110,814,573 bp
  • A to T, chromosome 9 at 18,376,533 bp
  • T to C, chromosome 9 at 39,426,619 bp
  • T to C, chromosome 9 at 39,950,537 bp
  • A to G, chromosome 9 at 58,082,022 bp
  • T to C, chromosome 10 at 11,162,235 bp
  • A to G, chromosome 10 at 81,333,149 bp
  • T to C, chromosome 10 at 105,370,357 bp
  • A to T, chromosome 10 at 117,363,036 bp
  • T to C, chromosome 11 at 3,432,445 bp
  • C to T, chromosome 11 at 53,286,933 bp
  • C to T, chromosome 11 at 67,193,729 bp
  • A to G, chromosome 11 at 101,431,344 bp
  • A to G, chromosome 11 at 104,665,510 bp
  • T to G, chromosome 12 at 31,341,449 bp
  • C to T, chromosome 12 at 34,407,802 bp
  • TCATTGTCC to TCATTGTCCATTGTCC, chromosome 13 at 47,064,088 bp
  • A to G, chromosome 13 at 54,085,517 bp
  • T to A, chromosome 13 at 59,703,715 bp
  • A to G, chromosome 13 at 67,670,312 bp
  • A to G, chromosome 13 at 95,370,846 bp
  • A to G, chromosome 14 at 30,929,577 bp
  • A to G, chromosome 14 at 31,667,060 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to C, chromosome 16 at 19,515,246 bp
  • A to T, chromosome 16 at 32,755,891 bp
  • G to A, chromosome 16 at 32,766,942 bp
  • G to A, chromosome 16 at 36,031,221 bp
  • A to G, chromosome 16 at 59,554,678 bp
  • C to T, chromosome 17 at 32,356,925 bp
  • T to A, chromosome 17 at 35,060,211 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2225 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040226-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.