Strain Name:
C57BL/6J-MtgxR2226Btlr/Mmmh
Stock Number:
040227-MU
Citation ID:
RRID:MMRRC_040227-MU
Other Names:
R2226 (G1), C57BL/6J-MtgxR2226Btlr
Major Collection:

Strain Information

Aggf1
Name: angiogenic factor with G patch and FHA domains 1
Synonyms: 2310029P06Rik, VG5Q, 2010009L17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66549
Homologene: 41220
Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Ptch1
Name: patched 1
Synonyms: Ptc, wig, Patched 1, A230106A15Rik, Ptc1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Spns1
Name: SPNS lysolipid transporter 1, lysophospholipid
Synonyms: 2210013K02Rik, spinster homolog
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73658
Homologene: 41530
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Slc25a33
Name: solute carrier family 25, member 33
Synonyms: Pnc1, 5730438N18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70556
Homologene: 57076
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: 4930523M17Rik, 1500002J22Rik, repro8, eIF4GII, G1-419-52
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: B630009I04Rik, ASC1p200, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Kpna1
Name: karyopherin subunit alpha 1
Synonyms: importin alpha 5, m-importin-alpha-S1, NPI1, Rch2, mSRP1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16646
HGNC: HGNC:6394
Homologene: 55642
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269470
Homologene: 4937
Sfxn1
Name: sideroflexin 1
Synonyms: A930015P12Rik, 2810002O05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Aen
Name: apoptosis enhancing nuclease
Synonyms: Isg20l1, 2700083B06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68048
Homologene: 23382
Pcx
Name: pyruvate carboxylase
Synonyms: Pc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18563
VEGA: 19
HGNC: HGNC:8636
Homologene: 5422
Zfp738
Name: zinc finger protein 738
Synonyms: 6720487G11Rik, 3830402I07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408068
Homologene: 133713
Kirrel2
Name: kirre like nephrin family adhesion molecule 2
Synonyms: C330019F22Rik, NEPH3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243911
Homologene: 35249
Atp1b3
Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11933
HGNC: HGNC:806
Homologene: 37510
Srsf6
Name: serine and arginine-rich splicing factor 6
Synonyms: Sfrs6, 1210001E11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67996
Homologene: 110783
Hnrnpul2
Name: heterogeneous nuclear ribonucleoprotein U-like 2
Synonyms: Hnrpul2, 1110031M08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 68693
VEGA: 19
Homologene: 28583
Hectd3
Name: HECT domain E3 ubiquitin protein ligase 3
Synonyms: 1700064K09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76608
Homologene: 18426
Marchf7
Name: membrane associated ring-CH-type finger 7
Synonyms: March7, Axot, Gtrgeo17
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57438
Homologene: 10753
Mtus1
Name: mitochondrial tumor suppressor 1
Synonyms: MTSG1, Atip1, B430305I03Rik, MD44
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102103
Homologene: 100292
Ppwd1
Name: peptidylprolyl isomerase domain and WD repeat containing 1
Synonyms: 4632422M10Rik, A330090G21Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238831
VEGA: 13
Homologene: 9099
Ptpn4
Name: protein tyrosine phosphatase, non-receptor type 4
Synonyms: hPTP-MEG, PTPMEG, testis-enriched phosphatase, TEP, protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte), TEP/mPTPMEG
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19258
HGNC: HGNC:9656
Homologene: 2120
Krt19
Name: keratin 19
Synonyms: K19, Krt1-19, cytokeratin19, EndoC, cytokeratin-19, cytokeratin 19, Krt-1.19
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16669
HGNC: HGNC:6436
Homologene: 1713
Ar
Name: androgen receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11835
HGNC: HGNC:644
Homologene: 28
Trpc6
Name: transient receptor potential cation channel, subfamily C, member 6
Synonyms: Trrp6, mtrp6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22068
VEGA: 9
Homologene: 37944
Mthfd2l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like
Synonyms: 1110019K23Rik, C630010D07Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 665563
Homologene: 28295
Miox
Name: myo-inositol oxygenase
Synonyms: 0610009I10Rik, C85427, Aldrl6, RSOR
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56727
Homologene: 9732
Nkpd1
Name: NTPase, KAP family P-loop domain containing 1
Synonyms: 2310015G09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69547
Homologene: 18876
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: fibrocystin L, PKHDL1, D86 mRNA
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 192190
Homologene: 16332
Vmn2r100
Name: vomeronasal 2, receptor 100
Synonyms: EG627537
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627537
Homologene: 129750
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 170571
Homologene: 24912
Iigp1
Name: interferon inducible GTPase 1
Synonyms: 2900074L10Rik, Irga6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 60440
VEGA: 18
Atg9b
Name: autophagy related 9B
Synonyms: eONE, LOC213948, Apg9l2, Nos3as
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 213948
Homologene: 72638
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78016
Homologene: 15814
Nsun7
Name: NOL1/NOP2/Sun domain family, member 7
Synonyms: 4921525L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70918
Homologene: 11653
Elfn2
Name: leucine rich repeat and fibronectin type III, extracellular 2
Synonyms: Lrrc62
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 207393
VEGA: 15
Homologene: 18835
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 59308
Homologene: 9438
Sgsm3
Name: small G protein signaling modulator 3
Synonyms: 1810012I01Rik, Rutbc3, CIP85
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105835
Homologene: 9249
Semp2l1
Name: SUMO/sentrin specific peptidase 2-like 1
Synonyms: Gm5415
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 408191
Homologene: 130042
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627805
Homologene: 129678
Slc22a19
Name: solute carrier family 22 (organic anion transporter), member 19
Synonyms: Slc22a9, Oat5, D630043A20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 207151
Homologene: 133125
Nxph3
Name: neurexophilin 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104079
HGNC: HGNC:8077
Homologene: 5227
Fam75d3
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Thoc2l
Name: THO complex subunit 2-like
Synonyms: BC005561, Gm3179
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100042165
Or2l5
Name: olfactory receptor family 2 subfamily L member 5
Synonyms: Olfr167, GA_x54KRFPKG5P-15963726-15962788, MOR272-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258937
Homologene: 78689
Ndufaf5
Name: NADH:ubiquinone oxidoreductase complex assembly factor 5
Synonyms: 2310003L22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69487
Homologene: 11474
Gabrg2
Name: gamma-aminobutyric acid type A receptor, subunit gamma 2
Synonyms: gamma2, GABAA-R, Gabrg-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14406
HGNC: HGNC:4087
Homologene: 22443
P2rx2
Name: purinergic receptor P2X, ligand-gated ion channel, 2
Synonyms: P2x2, P2X2a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231602
Homologene: 14251
Pank1
Name: pantothenate kinase 1
Synonyms: Pank1b, 4632412I06Rik, 5430426F23Rik, Pank1, Pank1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 75735
VEGA: 19
HGNC: HGNC:8598
Homologene: 56979
Krtap4-24
Name: keratin associated protein 4-24
Synonyms: Gm11555
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 546511
Or51a5
Name: olfactory receptor family 51 subfamily A member 5
Synonyms: MOR8-7, Olfr586, GA_x6K02T2PBJ9-5836380-5835439, MOR8-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259115
Homologene: 131143
Aadacl3
Name: arylacetamide deacetylase like 3
Synonyms: LOC230883
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230883
Homologene: 28426
Or7g19
Name: olfactory receptor family 7 subfamily G member 19
Synonyms: GA_x6K02T2PVTD-12687800-12688738, MOR153-1, Olfr832
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258075
HGNC: HGNC:8467
Homologene: 128143
Efhb
Name: EF hand domain family, member B
Synonyms: 4921525D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211482
VEGA: 17
Homologene: 16982
Or5aq1
Name: olfactory receptor family 5 subfamily AQ member 1
Synonyms: GA_x6K02T2Q125-48621299-48620361, Olfr1110, MOR172-4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258765
Ttc12
Name: tetratricopeptide repeat domain 12
Synonyms: E330017O07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235330
Homologene: 32375
Or4k1
Name: olfactory receptor family 4 subfamily K member 1
Synonyms: MOR246-1P, Olfr728, GA_x6K02T2PMLR-5831021-5830086
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258039
Homologene: 74224
Dgkk
Name: diacylglycerol kinase kappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 331374
Homologene: 45867
Tesl2
Name: testin LIM domain protein like 2
Synonyms: Gm4985
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245376
CU019598.2
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 32,545,853 bp
  • A to G, chromosome 1 at 54,364,925 bp
  • G to T, chromosome 1 at 118,964,563 bp
  • C to T, chromosome 1 at 119,682,785 bp
  • A to G, chromosome 2 at 60,229,846 bp
  • A to C, chromosome 2 at 87,136,246 bp
  • T to C, chromosome 2 at 140,188,860 bp
  • G to A, chromosome 2 at 162,931,699 bp
  • A to G, chromosome 3 at 100,146,791 bp
  • T to C, chromosome 3 at 137,404,017 bp
  • T to C, chromosome 4 at 116,995,689 bp
  • T to C, chromosome 4 at 138,175,837 bp
  • G to A, chromosome 4 at 141,321,237 bp
  • A to T, chromosome 4 at 144,463,725 bp
  • A to T, chromosome 4 at 149,753,849 bp
  • A to G, chromosome 5 at 24,386,395 bp
  • T to A, chromosome 5 at 66,261,219 bp
  • G to T, chromosome 5 at 90,948,834 bp
  • T to A, chromosome 5 at 104,519,420 bp
  • A to G, chromosome 5 at 110,342,879 bp
  • A to T, chromosome 7 at 19,519,820 bp
  • T to C, chromosome 7 at 30,454,154 bp
  • C to A, chromosome 7 at 78,902,451 bp
  • A to G, chromosome 7 at 103,121,908 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • C to T, chromosome 8 at 41,082,775 bp
  • A to G, chromosome 8 at 112,815,488 bp
  • A to G, chromosome 9 at 8,649,542 bp
  • A to T, chromosome 9 at 18,944,881 bp
  • A to G, chromosome 9 at 49,441,835 bp
  • A to G, chromosome 9 at 96,343,276 bp
  • A to G, chromosome 10 at 50,754,052 bp
  • A to T, chromosome 11 at 41,971,908 bp
  • T to C, chromosome 11 at 95,514,164 bp
  • T to G, chromosome 11 at 99,649,759 bp
  • T to C, chromosome 11 at 100,141,575 bp
  • A to G, chromosome 13 at 54,085,517 bp
  • T to A, chromosome 13 at 59,703,715 bp
  • A to G, chromosome 13 at 63,513,671 bp
  • A to G, chromosome 13 at 67,670,312 bp
  • A to G, chromosome 13 at 95,370,846 bp
  • A to G, chromosome 13 at 104,217,245 bp
  • A to G, chromosome 14 at 50,140,619 bp
  • T to C, chromosome 15 at 44,512,792 bp
  • A to G, chromosome 15 at 78,674,243 bp
  • A to G, chromosome 15 at 81,003,868 bp
  • C to A, chromosome 15 at 89,335,662 bp
  • T to C, chromosome 16 at 19,515,246 bp
  • G to A, chromosome 16 at 36,031,221 bp
  • A to G, chromosome 17 at 19,522,372 bp
  • A to T, chromosome 17 at 20,481,033 bp
  • T to C, chromosome 17 at 25,385,943 bp
  • A to T, chromosome 17 at 53,462,429 bp
  • A to T, chromosome 18 at 60,389,888 bp
  • T to C, chromosome 19 at 4,617,998 bp
  • C to T, chromosome 19 at 7,683,850 bp
  • T to A, chromosome 19 at 8,824,985 bp
  • A to T, chromosome 19 at 34,827,363 bp
  • T to A, chromosome X at 6,875,248 bp
  • T to A, chromosome X at 23,958,934 bp
  • A to T, chromosome X at 34,237,275 bp
  • T to A, chromosome X at 98,151,331 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2226 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040227-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.