Strain Name:
C57BL/6J-MtgxR2231Btlr/Mmmh
Stock Number:
040232-MU
Citation ID:
RRID:MMRRC_040232-MU
Other Names:
R2231 (G1), C57BL/6J-MtgxR2231Btlr
Major Collection:

Gene Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Trdmt1
Name: tRNA aspartic acid methyltransferase 1
Synonyms: Rnmt2, Dnmt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13434
HGNC: HGNC:2977
Homologene: 3249
Pou5f1
Name: POU domain, class 5, transcription factor 1
Synonyms: Oct4, Oct3/4, Oct-3/4, Otf4, Otf3, Otf3g, Otf3-rs7, Otf-4, Otf-3, Oct-4, Oct-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18999
Homologene: 8422
Cdk8
Name: cyclin-dependent kinase 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 264064
HGNC: HGNC:1779
Homologene: 55565
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243272
Homologene: 10055
Metap1
Name: methionyl aminopeptidase 1
Synonyms: 1700029C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 75624
Homologene: 6488
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: 1300012C07Rik, Nedd4-2, Nedd4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Ppp3r1
Name: protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Synonyms: PP2B beta 1, Cnb1, CaNB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19058
HGNC: HGNC:9317
Homologene: 68099
Coch
Name: cochlin
Synonyms: Coch-5B2, D12H14S564E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12810
VEGA: 12
HGNC: HGNC:2180
Homologene: 20868
Slc6a11
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 11
Synonyms: GAT4, D930045G19Rik, E130202I16Rik, Gabt4, Gat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243616
Homologene: 129935
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Enoph1
Name: enolase-phosphatase 1
Synonyms: 2310057D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67870
Homologene: 5790
Tcerg1
Name: transcription elongation regulator 1 (CA150)
Synonyms: p144, ca150, Taf2s, 2410022J09Rik, 2900090C16Rik, Fbp28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 56070
Homologene: 4879
Cblb
Name: Casitas B-lineage lymphoma b
Synonyms: Cbl-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208650
VEGA: 16
HGNC: HGNC:1542
Homologene: 15856
Usp33
Name: ubiquitin specific peptidase 33
Synonyms: Vdu1, 9830169D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170822
Homologene: 8996
Nup153
Name: nucleoporin 153
Synonyms: B130015D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218210
HGNC: HGNC:8062
Homologene: 68442
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, Myhc-a, alpha cardiac MHC, cardiomyopathy, hypertrophic 1, Myhca, A830009F23Rik, alpha-MHC, alphaMHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1537Clo, b2b1565Clo, b2b1154Clo, b2b1134Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, D830007G01Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Myl3
Name: myosin, light polypeptide 3
Synonyms: MLC1v, MLC1s, Mylc, slow skeletal, ventricular, alkali
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17897
HGNC: HGNC:7584
Homologene: 20099
Tbx3
Name: T-box 3
Synonyms: D5Ertd189e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21386
Homologene: 4371
Kif21a
Name: kinesin family member 21A
Synonyms: N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16564
VEGA: 15
Homologene: 56761
Entpd7
Name: ectonucleoside triphosphate diphosphohydrolase 7
Synonyms: LALP1, 2810003F23Rik, 1810020C02Rik, 1810012B13Rik, Lysal2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 93685
Homologene: 122202
Arsa
Name: arylsulfatase A
Synonyms: As-2, ASA, As2, AS-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11883
HGNC: HGNC:713
Homologene: 20138
Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Fam227a
Name: family with sequence similarity 227, member A
Synonyms: 4933432B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75729
Homologene: 123434
Oaz3
Name: ornithine decarboxylase antizyme 3
Synonyms: ornithine decarboxylase antizyme in testis, Oaz-t, AZ-3, antizyme 3, AZ3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53814
HGNC: HGNC:8097
Homologene: 9410
Prr5
Name: proline rich 5 (renal)
Synonyms: C030017C09Rik, Protor-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 109270
Homologene: 17132
Pacs2
Name: phosphofurin acidic cluster sorting protein 2
Synonyms: 6720425G15Rik, Pacs1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217893
VEGA: 12
Homologene: 15016
Usb1
Name: U6 snRNA biogenesis 1
Synonyms: AA960436
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101985
Homologene: 11611
Hscb
Name: HscB iron-sulfur cluster co-chaperone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100900
Homologene: 13945
Alkbh6
Name: alkB homolog 6
Synonyms: Abh6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233065
Homologene: 15555
Gal3st1
Name: galactose-3-O-sulfotransferase 1
Synonyms: galactosylceramide sulfotransferase, 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase, GalCer sulfotransferase, Gcst, Cst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53897
Homologene: 3574
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20392
Homologene: 31205
Plekhd1
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1
Synonyms: 3830431G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217682
Homologene: 15090
Zw10
Name: zw10 kinetochore protein
Synonyms: MmZw10, 6330566F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 26951
VEGA: 9
Homologene: 37959
Or5b12b
Name: olfactory receptor family 5 subfamily B member 12B
Synonyms: GA_x6K02T2RE5P-3213352-3214296, MOR202-7, Olfr1445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258694
Homologene: 133606
Cyp2c68
Name: cytochrome P450, family 2, subfamily c, polypeptide 68
Synonyms: 9030012A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 433247
Homologene: 74936
Eif2b5
Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224045
HGNC: HGNC:3261
Homologene: 2903
Or4c3d
Name: olfactory receptor family 4 subfamily C member 3D
Synonyms: A16, MOR235-1, GA_x6K02T2Q125-51484508-51483600, Olfr140
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57272
Homologene: 41379
Ankrd63
Name: ankyrin repeat domain 63
Synonyms: LOC383787, Gm1337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 383787
Homologene: 19774
Spag1
Name: sperm associated antigen 1
Synonyms: TPR-containing protein involved in spermatogenesis, tpis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26942
VEGA: 15
Homologene: 8081
Ssna1
Name: SS nuclear autoantigen 1
Synonyms: 1190004J23Rik, 1110003H09Rik, NA14, N14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68475
Homologene: 2768
Actr1b
Name: ARP1 actin-related protein 1B, centractin beta
Synonyms: Arp1b, 2310066K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226977
HGNC: HGNC:168
Homologene: 101541
Cyp2e1
Name: cytochrome P450, family 2, subfamily e, polypeptide 1
Synonyms: Cyp2e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13106
HGNC: HGNC:2631
Homologene: 68089
Zfp92
Name: zinc finger protein 92
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22754
Homologene: 69050
L1cam
Name: L1 cell adhesion molecule
Synonyms: L1, CD171, NCAM-L1, L1-NCAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 16728
HGNC: HGNC:6470
Homologene: 20128
Gm10650
Name: predicted gene 10650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Pdk3
Name: pyruvate dehydrogenase kinase, isoenzyme 3
Synonyms: 2610001M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236900
HGNC: HGNC:8811
Homologene: 55897
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 36,700,359 bp
  • A to T, chromosome 2 at 13,525,625 bp
  • G to T, chromosome 2 at 25,272,007 bp
  • A to T, chromosome 2 at 76,944,153 bp
  • A to T, chromosome 2 at 90,052,225 bp
  • A to G, chromosome 2 at 118,703,365 bp
  • T to C, chromosome 3 at 94,434,539 bp
  • A to G, chromosome 3 at 128,039,763 bp
  • A to G, chromosome 3 at 138,466,379 bp
  • C to T, chromosome 3 at 152,373,386 bp
  • C to T, chromosome 5 at 100,040,277 bp
  • G to T, chromosome 5 at 110,839,488 bp
  • C to A, chromosome 5 at 119,677,524 bp
  • A to T, chromosome 5 at 124,405,704 bp
  • A to T, chromosome 5 at 146,231,604 bp
  • T to C, chromosome 6 at 4,730,066 bp
  • A to G, chromosome 6 at 114,194,629 bp
  • G to A, chromosome 7 at 30,312,590 bp
  • T to C, chromosome 7 at 140,764,914 bp
  • T to G, chromosome 8 at 95,344,046 bp
  • C to T, chromosome 9 at 49,064,121 bp
  • T to C, chromosome 9 at 110,767,911 bp
  • T to C, chromosome 9 at 119,633,850 bp
  • T to C, chromosome 11 at 3,998,282 bp
  • G to A, chromosome 11 at 17,193,115 bp
  • G to A, chromosome 12 at 51,602,865 bp
  • T to C, chromosome 12 at 80,721,951 bp
  • G to A, chromosome 12 at 113,063,367 bp
  • A to G, chromosome 13 at 46,709,627 bp
  • T to C, chromosome 14 at 54,961,842 bp
  • A to G, chromosome 14 at 61,205,929 bp
  • G to A, chromosome 15 at 28,408,417 bp
  • A to G, chromosome 15 at 36,191,167 bp
  • T to A, chromosome 15 at 79,615,381 bp
  • T to C, chromosome 15 at 84,702,780 bp
  • T to C, chromosome 15 at 89,475,722 bp
  • G to A, chromosome 15 at 90,985,362 bp
  • T to C, chromosome 16 at 20,504,770 bp
  • T to A, chromosome 16 at 52,194,272 bp
  • C to T, chromosome 17 at 25,397,858 bp
  • A to G, chromosome 17 at 35,510,062 bp
  • A to G, chromosome 18 at 42,524,244 bp
  • A to T, chromosome 18 at 63,145,072 bp
  • C to T, chromosome 18 at 65,203,961 bp
  • A to G, chromosome 19 at 12,883,949 bp
  • T to A, chromosome 19 at 39,699,360 bp
  • T to C, chromosome 19 at 43,721,816 bp
  • G to T, chromosome X at 73,422,752 bp
  • T to A, chromosome X at 73,861,341 bp
  • G to T, chromosome X at 93,813,998 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2231 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040232-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.