Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR2245Btlr/Mmmh
Stock Number:
040245-MU
Citation ID:
RRID:MMRRC_040245-MU
Other Names:
R2245 (G1), C57BL/6J-MtgxR2245Btlr
Major Collection:

Gene Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Jarid2
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 2500001H09Rik, 5730417B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74737
Homologene: 32282
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: Rpo2-1, 220kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Wdr7
Name: WD repeat domain 7
Synonyms: TGF-beta resistance associated gene, TRAG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 104082
VEGA: 18
Homologene: 11408
Brd1
Name: bromodomain containing 1
Synonyms: 1110059H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223770
HGNC: HGNC:1102
Homologene: 40956
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74081
Homologene: 8879
Tomm22
Name: translocase of outer mitochondrial membrane 22
Synonyms: Tom22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223696
Homologene: 10638
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 20658
Homologene: 10551
Otof
Name: otoferlin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Plod2
Name: procollagen lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms: Plod-2, lysyl hydroxylase 2, LH2, D530025C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 26432
HGNC: HGNC:9082
Homologene: 22681
Csnk1d
Name: casein kinase 1, delta
Synonyms: 1200006A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 104318
HGNC: HGNC:2452
Homologene: 74841
Unkl
Name: unkempt family like zinc finger
Synonyms: 1300004G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 74154
Homologene: 62673
Myo15
Name: myosin XV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 104271
Homologene: 12837
Adamtsl3
Name: ADAMTS-like 3
Synonyms: punctin-2, 9230119C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269959
Homologene: 18912
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Ptprh
Name: protein tyrosine phosphatase, receptor type, H
Synonyms: SAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 545902
HGNC: HGNC:9672
Homologene: 37693
Specc1
Name: sperm antigen with calponin homology and coiled-coil domains 1
Synonyms: B230396K10Rik, 2810012G08Rik, Cytsb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 432572
Homologene: 45157
Ephb1
Name: Eph receptor B1
Synonyms: C130099E04Rik, Hek6, Cek6, Net, Elkh, Elk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270190
HGNC: HGNC:3392
Homologene: 20936
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225638
Homologene: 50475
Prpf40b
Name: pre-mRNA processing factor 40B
Synonyms: 2610317D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 54614
Homologene: 81828
Olfr1216
Name: olfactory receptor 1216
Synonyms: MOR233-9, GA_x6K02T2Q125-50494588-50493653
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258895
HGNC: null
Homologene: 74241
Ccdc158
Name: coiled-coil domain containing 158
Synonyms: 4932413O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 320696
Homologene: 18560
Tmem237
Name: transmembrane protein 237
Synonyms: LOC381259, Als2cr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381259
Homologene: 17570
Vmn2r106
Name: vomeronasal 2, receptor 106
Synonyms: EG224576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224576
HGNC: null
Homologene: 135824
Slc19a3
Name: solute carrier family 19, member 3
Synonyms: ThTr2, A230084E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 80721
Homologene: 23530
Olfr745
Name: olfactory receptor 745
Synonyms: MOR106-11, GA_x6K02T2PMLR-6361495-6362481
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258296
Homologene: 27116
Vmn2r71
Name: vomeronasal 2, receptor 71
Synonyms: EG233445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233445
HGNC: null
Homologene: 115466
Olfr1355
Name: olfactory receptor 1355
Synonyms: GA_x6K02T2QGN0-2932609-2931677, MOR139-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 257734
HGNC: null
Homologene: 136441
Ddah2
Name: dimethylarginine dimethylaminohydrolase 2
Synonyms: Clone 7u, NG30, Ddah, G6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 51793
HGNC: HGNC:2716
Homologene: 8510
Rdh16f2
Name: RDH16 family member 2
Synonyms: BC089597
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216454
Homologene: 129514
Myadml2
Name: myeloid-associated differentiation marker-like 2
Synonyms: 1110012N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 68515
Homologene: 19528
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 59,108,704 bp
  • T to C, chromosome 1 at 83,013,970 bp
  • C to A, chromosome 1 at 155,879,020 bp
  • A to C, chromosome 2 at 76,829,639 bp
  • A to T, chromosome 2 at 89,013,149 bp
  • T to C, chromosome 5 at 30,370,207 bp
  • A to G, chromosome 5 at 92,609,952 bp
  • G to A, chromosome 6 at 84,186,509 bp
  • T to C, chromosome 7 at 4,573,346 bp
  • A to G, chromosome 7 at 82,450,100 bp
  • T to A, chromosome 7 at 85,624,180 bp
  • T to C, chromosome 7 at 92,665,872 bp
  • G to A, chromosome 7 at 141,638,400 bp
  • A to G, chromosome 8 at 33,571,496 bp
  • T to C, chromosome 9 at 92,571,246 bp
  • A to C, chromosome 9 at 101,996,774 bp
  • A to G, chromosome 10 at 78,879,931 bp
  • A to G, chromosome 10 at 127,876,276 bp
  • T to C, chromosome 11 at 60,509,099 bp
  • T to C, chromosome 11 at 62,131,887 bp
  • A to G, chromosome 11 at 68,385,294 bp
  • A to G, chromosome 11 at 69,735,183 bp
  • A to T, chromosome 11 at 120,647,830 bp
  • A to G, chromosome 11 at 120,972,403 bp
  • T to A, chromosome 13 at 44,906,276 bp
  • T to A, chromosome 14 at 50,642,605 bp
  • T to C, chromosome 15 at 79,671,013 bp
  • A to G, chromosome 15 at 88,689,860 bp
  • A to G, chromosome 15 at 99,305,166 bp
  • G to T, chromosome 16 at 91,647,960 bp
  • T to C, chromosome 17 at 20,268,161 bp
  • T to A, chromosome 17 at 25,205,587 bp
  • T to C, chromosome 17 at 35,061,585 bp
  • A to T, chromosome 18 at 63,924,909 bp
  • A to G, chromosome 18 at 65,305,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2245 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040245-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.