Strain Name:
C57BL/6J-MtgxR2248Btlr/Mmmh
Stock Number:
040248-MU
Citation ID:
RRID:MMRRC_040248-MU
Other Names:
R2248 (G1), C57BL/6J-MtgxR2248Btlr
Major Collection:

Gene Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77569
Homologene: 18953
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: D230004K18Rik, Megalin, Gp330, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Arl8b
Name: ADP-ribosylation factor-like 8B
Synonyms: Arl10c, 3100002J04Rik, 2610313E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67166
Homologene: 10056
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, Pam, C130061D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66185
Homologene: 41043
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Plk1
Name: polo like kinase 1
Synonyms: STPK13, Plk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18817
HGNC: HGNC:9077
Homologene: 3690
Ptcd3
Name: pentatricopeptide repeat domain 3
Synonyms: 2810422B04Rik, 2610034F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69956
Homologene: 41211
Cdhr1
Name: cadherin-related family member 1
Synonyms: Prcad, Pcdh21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 170677
VEGA: 14
Homologene: 13215
Dcc
Name: deleted in colorectal carcinoma
Synonyms: Igdcc1, C030036D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13176
HGNC: HGNC:2701
Homologene: 21081
Pms2
Name: PMS1 homolog2, mismatch repair system component
Synonyms: mismatch repair, DNA mismatch repair
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18861
HGNC: HGNC:9122
Homologene: 133560
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Homer1
Name: homer scaffolding protein 1
Synonyms: Ves-1, PSD-Zip45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26556
Homologene: 3155
Eva1c
Name: eva-1 homolog C (C. elegans)
Synonyms: Fam176c, 1700092M14Rik, 4931408A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70967
Homologene: 14383
Glipr1l1
Name: GLI pathogenesis-related 1 like 1
Synonyms: 1700011E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69286
VEGA: 10
Homologene: 83452
Trappc6b
Name: trafficking protein particle complex 6B
Synonyms: 5830498C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 78232
VEGA: 12
Homologene: 42466
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, tf, Ip3r3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Foxn3
Name: forkhead box N3
Synonyms: 5430426H20Rik, Ches1, Ches1l, HTLFL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71375
HGNC: HGNC:1928
Homologene: 135955
Ank
Name: progressive ankylosis
Synonyms: D15Ertd221e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11732
Homologene: 10664
Ppp2r5c
Name: protein phosphatase 2, regulatory subunit B', gamma
Synonyms: 2700063L20Rik, Band 8A, B56/PP2A gamma, 2610043M05Rik, D12Bwg0916e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26931
VEGA: 12
HGNC: HGNC:9311
Homologene: 128665
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Gpr152
Name: G protein-coupled receptor 152
Synonyms: LOC269053, A930009H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 269053
VEGA: 19
Homologene: 35474
Tspear
Name: thrombospondin type laminin G domain and EAR repeats
Synonyms: C330046G03Rik, ORF65
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 252974
HGNC: HGNC:1268
Homologene: 36972
Aldh1a2
Name: aldehyde dehydrogenase family 1, subfamily A2
Synonyms: Aldh1a7, retinaldehyde dehydrogenase, Raldh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19378
Homologene: 68368
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy3, hyrh, hy-3, 4930545D19Rik, 1700034M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: diminished cone electroretinogram, D12Ertd777e, dice, syne-2, nesprin-2, 6820443O06Rik, Cpfl8, Nesp2g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Ptprq
Name: protein tyrosine phosphatase, receptor type, Q
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Dpysl3
Name: dihydropyrimidinase-like 3
Synonyms: CRMP-4, Ulip, TUC4, Ulip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22240
HGNC: HGNC:3015
Homologene: 20361
Vwa7
Name: von Willebrand factor A domain containing 7
Synonyms: G7c, D17H6S56E-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27762
Homologene: 11895
Nav3
Name: neuron navigator 3
Synonyms: 4732483H20Rik, POMFIL1, Pomfil1p, unc53H3, 9630020C08Rik, steerin 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 229003
Homologene: 14118
Afp
Name: alpha fetoprotein
Synonyms: alpha-foetoprotein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11576
HGNC: HGNC:317
Homologene: 36278
Lgsn
Name: lengsin, lens protein with glutamine synthetase domain
Synonyms: Gluld1, lengsin, Lgs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 266744
Homologene: 9569
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Ifi207
Name: interferon activated gene 207
Synonyms: AI607873, Pyhin-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268527
Homologene: 8780
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: 1110001D15Rik, Vwa6, Dvwa, EG235580
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68553
Homologene: 130754
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Slco1c1
Name: solute carrier organic anion transporter family, member 1c1
Synonyms: Slc21a14, OATP-F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58807
Homologene: 23008
Flnc
Name: filamin C, gamma
Synonyms: 1110055E19Rik, actin binding protein 280, Fln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Bfsp1
Name: beaded filament structural protein 1, in lens-CP94
Synonyms: filensin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12075
HGNC: HGNC:1040
Homologene: 922
Abca2
Name: ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms: Abc2, D2H0S1474E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11305
HGNC: HGNC:32
Homologene: 55590
Clta
Name: clathrin, light polypeptide (Lca)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12757
HGNC: HGNC:2090
Homologene: 1384
Tsen54
Name: tRNA splicing endonuclease subunit 54
Synonyms: 0610034P02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76265
Homologene: 35476
Kif21b
Name: kinesin family member 21B
Synonyms: N-5 kinesin, 2610511N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16565
Homologene: 56868
Ano5
Name: anoctamin 5
Synonyms: Tmem16e, Gdd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233246
Homologene: 100071
Clca3b
Name: chloride channel accessory 3B
Synonyms: Clca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229927
Homologene: 135610
Lrrc31
Name: leucine rich repeat containing 31
Synonyms: E230002P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 320352
Frk
Name: fyn-related kinase
Synonyms: BSK/IYK, GTK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14302
VEGA: 10
HGNC: HGNC:3955
Homologene: 48065
Chst8
Name: carbohydrate sulfotransferase 8
Synonyms: 1500011J21Rik, GalNAc4ST-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68947
Homologene: 41483
Khnyn
Name: KH and NYN domain containing
Synonyms: 9130227C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219094
VEGA: 14
Homologene: 23611
H2-DMb1
Name: histocompatibility 2, class II, locus Mb1
Synonyms: H2-Mb1, H2-M beta1, H-2Mb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14999
HGNC: HGNC:4935
Homologene: 68231
Olfr1141
Name: olfactory receptor 1141
Synonyms: GA_x6K02T2Q125-49257818-49256883, MOR177-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258630
Homologene: 74080
Oas1c
Name: 2'-5' oligoadenylate synthetase 1C
Synonyms: Oasl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 114643
HGNC: HGNC:8086
Homologene: 110815
Tmod2
Name: tropomodulin 2
Synonyms: NTMOD, neural tropomodulin, N-Tmod
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 50876
VEGA: 9
Homologene: 22817
Alg6
Name: asparagine-linked glycosylation 6 (alpha-1,3,-glucosyltransferase)
Synonyms: E230028F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320438
Homologene: 6920
Olfr1254
Name: olfactory receptor 1254
Synonyms: MOR231-13, GA_x6K02T2Q125-51230155-51229211
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258468
Homologene: 103784
Olfr165
Name: olfactory receptor 165
Synonyms: MOR279-1, GA_x54KRFPKG5P-15855164-15854223
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258458
Homologene: 128262
9530077C05Rik
Name: RIKEN cDNA 9530077C05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68283
VEGA: 9
Homologene: 19280
AI593442
Name: expressed sequence AI593442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330941
VEGA: 9
Homologene: 52281
Mal2
Name: mal, T cell differentiation protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105853
VEGA: 15
Homologene: 14167
Sh3yl1
Name: Sh3 domain YSC-like 1
Synonyms: YSC84, Ray
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 24057
Homologene: 31662
Rab18
Name: RAB18, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 19330
VEGA: 18
Homologene: 40765
Gm6169
Name: predicted gene 6169
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 620648
VEGA: 13
Pole3
Name: polymerase (DNA directed), epsilon 3 (p17 subunit)
Synonyms: 1810034K18Rik, YBL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 59001
Homologene: 9694
Tas2r119
Name: taste receptor, type 2, member 119
Synonyms: mGR19, T2R19, Tas2r19, mt2r19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 57254
Homologene: 10480
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 31,203,526 bp
  • A to G, chromosome 1 at 66,623,206 bp
  • T to C, chromosome 1 at 88,256,754 bp
  • T to C, chromosome 1 at 136,172,966 bp
  • G to A, chromosome 1 at 173,736,470 bp
  • A to G, chromosome 2 at 25,433,464 bp
  • T to A, chromosome 2 at 69,511,010 bp
  • T to C, chromosome 2 at 87,753,943 bp
  • C to A, chromosome 2 at 89,789,180 bp
  • A to T, chromosome 2 at 143,827,652 bp
  • A to C, chromosome 2 at 181,233,433 bp
  • T to A, chromosome 3 at 30,689,901 bp
  • T to C, chromosome 3 at 144,825,219 bp
  • T to A, chromosome 4 at 11,518,927 bp
  • A to G, chromosome 4 at 44,012,852 bp
  • C to A, chromosome 4 at 62,525,013 bp
  • C to T, chromosome 4 at 99,738,207 bp
  • A to T, chromosome 5 at 62,849,900 bp
  • G to T, chromosome 5 at 67,044,399 bp
  • A to G, chromosome 5 at 90,501,570 bp
  • T to C, chromosome 5 at 120,802,861 bp
  • G to A, chromosome 5 at 143,916,506 bp
  • A to G, chromosome 6 at 29,451,401 bp
  • A to T, chromosome 6 at 71,894,285 bp
  • A to G, chromosome 6 at 108,783,343 bp
  • T to A, chromosome 6 at 141,546,689 bp
  • G to T, chromosome 7 at 34,748,172 bp
  • A to T, chromosome 7 at 51,593,789 bp
  • A to G, chromosome 7 at 122,168,821 bp
  • G to T, chromosome 8 at 110,578,203 bp
  • A to G, chromosome 9 at 22,444,114 bp
  • TGAGGAGGAGGAGGAGGA to TGAGGAGGAGGAGGA, chromosome 9 at 52,677,814 bp
  • A to T, chromosome 9 at 71,215,862 bp
  • G to T, chromosome 9 at 75,592,649 bp
  • T to G, chromosome 9 at 106,079,959 bp
  • C to A, chromosome 10 at 34,608,531 bp
  • A to G, chromosome 10 at 77,873,269 bp
  • A to T, chromosome 10 at 107,643,070 bp
  • T to A, chromosome 10 at 109,696,227 bp
  • A to G, chromosome 10 at 112,062,287 bp
  • T to C, chromosome 11 at 68,277,572 bp
  • A to G, chromosome 11 at 115,815,406 bp
  • T to A, chromosome 12 at 16,680,378 bp
  • T to A, chromosome 12 at 30,942,870 bp
  • G to T, chromosome 12 at 51,806,471 bp
  • T to C, chromosome 12 at 59,050,381 bp
  • C to T, chromosome 12 at 76,096,904 bp
  • T to C, chromosome 12 at 78,454,821 bp
  • T to C, chromosome 12 at 99,196,556 bp
  • T to C, chromosome 12 at 110,485,923 bp
  • T to C, chromosome 13 at 93,346,709 bp
  • C to A, chromosome 13 at 97,098,898 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • C to G, chromosome 14 at 37,081,377 bp
  • T to A, chromosome 14 at 55,886,738 bp
  • A to G, chromosome 14 at 61,212,802 bp
  • T to A, chromosome 14 at 103,169,859 bp
  • T to A, chromosome 15 at 27,562,711 bp
  • T to C, chromosome 15 at 32,178,151 bp
  • T to A, chromosome 15 at 54,588,336 bp
  • A to G, chromosome 16 at 19,407,194 bp
  • A to G, chromosome 16 at 90,831,325 bp
  • A to T, chromosome 17 at 27,115,059 bp
  • T to A, chromosome 17 at 34,159,469 bp
  • G to A, chromosome 17 at 35,019,043 bp
  • T to A, chromosome 18 at 6,788,629 bp
  • T to C, chromosome 18 at 43,358,293 bp
  • G to T, chromosome 18 at 71,826,168 bp
  • T to C, chromosome 19 at 4,143,806 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2248 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040248-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.