Strain Name:
C57BL/6J-MtgxR2252Btlr/Mmmh
Stock Number:
040252-MU
Citation ID:
RRID:MMRRC_040252-MU
Other Names:
R2252 (G1), C57BL/6J-MtgxR2252Btlr
Major Collection:

Gene Information

Septin4
Name: septin 4
Synonyms: cell division control-related protein 2b, Pnutl2, Bh5, septin H5, Gm11492, ARTS, Sept4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 109241
Homologene: 81861
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16396
Homologene: 88442
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75560
Homologene: 38779
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70097
Homologene: 69182
Pcif1
Name: phosphorylated CTD interacting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228866
Homologene: 11134
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Cwc27
Name: CWC27 spliceosome-associated protein
Synonyms: NY-CO-10, 3110009E13Rik, Sdccag10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67285
Homologene: 21192
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93742
Homologene: 10489
Kif13b
Name: kinesin family member 13B
Synonyms: GAKIN, N-3 kinesin, C130021D12Rik, 5330429L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16554
VEGA: 14
Homologene: 9073
Kpna1
Name: karyopherin (importin) alpha 1
Synonyms: mSRP1, m-importin-alpha-S1, Rch2, NPI1, importin alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16646
HGNC: HGNC:6394
Homologene: 55642
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Casp3
Name: caspase 3
Synonyms: CPP32, Apopain, Yama, Caspase-3, A830040C14Rik, CC3, mldy, AC-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12367
HGNC: HGNC:1504
Homologene: 37912
Secisbp2l
Name: SECIS binding protein 2-like
Synonyms: 3110001I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70354
Homologene: 18923
Stim2
Name: stromal interaction molecule 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 116873
Homologene: 32490
Zfp472
Name: zinc finger protein 472
Synonyms: Krim-1, Krim-1B, Krim-1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224691
Homologene: 77422
Iars2
Name: isoleucine-tRNA synthetase 2, mitochondrial
Synonyms: 2010002H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381314
Homologene: 7118
B3gnt3
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Synonyms: 2210008L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72297
Homologene: 113516
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: acetyl-CoA carboxylase, Acc1, LOC327983, Acac, A530025K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Upf2
Name: UPF2 regulator of nonsense transcripts homolog (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 326622
Homologene: 6101
Nrip1
Name: nuclear receptor interacting protein 1
Synonyms: RIP140, 6030458L20Rik, 8430438I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268903
HGNC: HGNC:8001
Homologene: 2606
Khdrbs1
Name: KH domain containing, RNA binding, signal transduction associated 1
Synonyms: p62, Sam68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20218
Homologene: 4781
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Cul2
Name: cullin 2
Synonyms: 1300003D18Rik, 4932411N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71745
HGNC: HGNC:2552
Homologene: 2662
Gzf1
Name: GDNF-inducible zinc finger protein 1
Synonyms: 8430437G08Rik, Zfp336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74533
Homologene: 11200
Ccdc171
Name: coiled-coil domain containing 171
Synonyms: 4930418J05Rik, A330015D16Rik, 4930473A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320226
Homologene: 27942
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381591
Homologene: 135709
Spink5
Name: serine peptidase inhibitor, Kazal type 5
Synonyms: LEKT1, 2310065D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72432
Homologene: 4987
Cabp1
Name: calcium binding protein 1
Synonyms: caldendrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29867
HGNC: HGNC:1384
Homologene: 99790
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Tea, Atrc2, Cat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11988
Homologene: 20659
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Enpep
Name: glutamyl aminopeptidase
Synonyms: Bp-1/6C3, APA, Ly-51, Ly51, 6030431M22Rik, aminopeptidase-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Cd180
Name: CD180 antigen
Synonyms: RP105, Ly78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17079
HGNC: HGNC:6726
Homologene: 4077
Dgkg
Name: diacylglycerol kinase, gamma
Synonyms: Dagk3, E430001K23Rik, 2900055E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 110197
HGNC: HGNC:2853
Homologene: 1029
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Cspg4
Name: chondroitin sulfate proteoglycan 4
Synonyms: NG2, AN2, 4732461B14Rik, Cspg4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 121021
VEGA: 9
HGNC: HGNC:2466
Homologene: 20445
Vmn2r18
Name: vomeronasal 2, receptor 18
Synonyms: EG632671
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 632671
Serping1
Name: serine (or cysteine) peptidase inhibitor, clade G, member 1
Synonyms: C1nh, C1 inhibitor, C1INH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12258
HGNC: HGNC:1228
Homologene: 44
Ubxn8
Name: UBX domain protein 8
Synonyms: Rep8h, Rep-8, D0H8S2298E, Ubxd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108159
Homologene: 4149
Foxa2
Name: forkhead box A2
Synonyms: Hnf-3b, Tcf-3b, Tcf3b, Hnf3b, HNF3beta, HNF3-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15376
HGNC: HGNC:5022
Homologene: 7762
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224552
Homologene: 115024
Vwa5a
Name: von Willebrand factor A domain containing 5A
Synonyms: BCSC-1, 5830475I06Rik, Loh11cr2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67776
HGNC: HGNC:6658
Homologene: 18222
Pkd1l2
Name: polycystic kidney disease 1 like 2
Synonyms: 1700126L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76645
Homologene: 124481
Drc1
Name: dynein regulatory complex subunit 1
Synonyms: LOC381738, Gm1060, b2b1654Clo, Ccdc164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381738
Homologene: 35977
Zfp536
Name: zinc finger protein 536
Synonyms: 9630010P11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243937
Homologene: 8813
1700129C05Rik
Name: RIKEN cDNA 1700129C05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67932
Homologene: 130749
Olfr1279
Name: olfactory receptor 1279
Synonyms: GA_x6K02T2Q125-72357646-72358581, MOR245-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258388
Homologene: 121520
Cdh15
Name: cadherin 15
Synonyms: Cdh14, Mcad, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Serpinb3b
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3B
Synonyms: Scca2-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 383548
Homologene: 131278
Gm9765
Name: predicted gene 9765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Olfr143
Name: olfactory receptor 143
Synonyms: MOR170-6, GA_x6K02T2PVTD-31941084-31942025, K18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258802
Homologene: 133627
Olfr958
Name: olfactory receptor 958
Synonyms: GA_x6K02T2PVTD-33247839-33246901, MOR224-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258327
VEGA: 9
Homologene: 27128
Cyp2c38
Name: cytochrome P450, family 2, subfamily c, polypeptide 38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13097
HGNC: HGNC:2622
Homologene: 117948
Pknox2
Name: Pbx/knotted 1 homeobox 2
Synonyms: Prep2, D230005H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208076
Homologene: 32527
Olfr988
Name: olfactory receptor 988
Synonyms: GA_x6K02T2Q125-46830591-46829662, MOR203-5P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258166
Homologene: 103791
Mrpl40
Name: mitochondrial ribosomal protein L40
Synonyms: Nlvcf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 18100
Homologene: 2800
Pcsk1
Name: proprotein convertase subtilisin/kexin type 1
Synonyms: PC3, prohormone convertase 1/3, Nec-1, Nec1, PC1, SPC3, Phpp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18548
HGNC: HGNC:8743
Homologene: 379
Tubb2b
Name: tubulin, beta 2B class IIB
Synonyms: 2410129E14Rik, brdp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 73710
VEGA: 13
Homologene: 49734
Thoc3
Name: THO complex 3
Synonyms: 2410044K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 73666
Homologene: 12505
Reep1
Name: receptor accessory protein 1
Synonyms: D6Ertd253e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 52250
Homologene: 41504
Zfp970
Name: zinc finger protein 970
Synonyms: Gm14420
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 628308
Homologene: 134324
Yjefn3
Name: YjeF N-terminal domain containing 3
Synonyms: LOC234365
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12041
Homologene: 37642
Lrrc52
Name: leucine rich repeat containing 52
Synonyms: 4930413P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240899
Homologene: 35430
Faap20
Name: Fanconi anemia core complex associated protein 20
Synonyms: 2610002J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67513
Homologene: 18793
Cldn24
Name: claudin 24
Synonyms: Gm10107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100039801
Homologene: 136011
BC048507
Name: cDNA sequence BC048507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408058
VEGA: 13
Homologene: 133712
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 107,155,478 bp
  • A to T, chromosome 1 at 167,466,368 bp
  • G to T, chromosome 1 at 171,516,872 bp
  • A to T, chromosome 1 at 185,325,494 bp
  • A to G, chromosome 2 at 5,961,460 bp
  • A to G, chromosome 2 at 49,257,686 bp
  • A to G, chromosome 2 at 84,769,851 bp
  • A to G, chromosome 2 at 85,353,858 bp
  • A to G, chromosome 2 at 111,306,310 bp
  • A to T, chromosome 2 at 118,718,780 bp
  • T to C, chromosome 2 at 118,723,765 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • A to G, chromosome 2 at 148,044,166 bp
  • T to C, chromosome 2 at 148,683,936 bp
  • T to A, chromosome 2 at 155,212,339 bp
  • A to G, chromosome 2 at 164,890,879 bp
  • T to A, chromosome 2 at 177,474,821 bp
  • A to T, chromosome 3 at 129,284,196 bp
  • T to A, chromosome 4 at 83,635,626 bp
  • A to G, chromosome 4 at 98,737,637 bp
  • A to G, chromosome 4 at 129,725,730 bp
  • A to C, chromosome 4 at 155,250,553 bp
  • G to T, chromosome 5 at 30,342,731 bp
  • G to A, chromosome 5 at 54,115,908 bp
  • A to T, chromosome 5 at 110,719,091 bp
  • T to A, chromosome 5 at 115,176,225 bp
  • T to C, chromosome 5 at 151,584,976 bp
  • T to A, chromosome 6 at 71,756,442 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • T to C, chromosome 7 at 37,479,389 bp
  • C to A, chromosome 7 at 127,905,418 bp
  • A to G, chromosome 8 at 33,635,364 bp
  • T to C, chromosome 8 at 40,905,621 bp
  • G to T, chromosome 8 at 46,637,955 bp
  • G to T, chromosome 8 at 47,822,328 bp
  • A to G, chromosome 8 at 48,292,277 bp
  • A to C, chromosome 8 at 69,889,445 bp
  • A to T, chromosome 8 at 71,692,818 bp
  • A to G, chromosome 8 at 95,763,186 bp
  • T to C, chromosome 8 at 117,057,438 bp
  • T to A, chromosome 8 at 122,857,422 bp
  • A to T, chromosome 8 at 127,610,599 bp
  • T to C, chromosome 9 at 36,910,520 bp
  • A to G, chromosome 9 at 38,253,830 bp
  • T to C, chromosome 9 at 38,728,080 bp
  • G to T, chromosome 9 at 39,549,977 bp
  • C to T, chromosome 9 at 56,898,046 bp
  • A to T, chromosome 10 at 8,729,977 bp
  • G to T, chromosome 11 at 84,371,532 bp
  • A to G, chromosome 11 at 87,589,811 bp
  • T to C, chromosome 11 at 103,501,467 bp
  • T to A, chromosome 13 at 34,130,215 bp
  • A to T, chromosome 13 at 54,467,833 bp
  • T to C, chromosome 13 at 67,863,507 bp
  • C to A, chromosome 13 at 75,126,726 bp
  • A to T, chromosome 13 at 102,706,398 bp
  • A to T, chromosome 13 at 104,631,729 bp
  • G to A, chromosome 14 at 59,142,612 bp
  • C to T, chromosome 14 at 64,742,298 bp
  • A to T, chromosome 14 at 67,784,812 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to C, chromosome 16 at 18,875,375 bp
  • A to T, chromosome 16 at 22,622,260 bp
  • T to C, chromosome 16 at 36,021,569 bp
  • T to C, chromosome 16 at 76,291,285 bp
  • A to C, chromosome 17 at 19,080,436 bp
  • A to T, chromosome 17 at 32,976,283 bp
  • A to T, chromosome 18 at 3,399,876 bp
  • G to T, chromosome 18 at 44,020,824 bp
  • T to A, chromosome 19 at 39,404,652 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2252 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040252-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.