Strain Name:
C57BL/6J-MtgxR2253Btlr/Mmmh
Stock Number:
040253-MU
Citation ID:
RRID:MMRRC_040253-MU
Other Names:
R2253 (G1), C57BL/6J-MtgxR2253Btlr
Major Collection:

Gene Information

Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319582
Homologene: 35306
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16396
Homologene: 88442
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75560
Homologene: 38779
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: 1100001C18Rik, A330076K04Rik, 2500002E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70097
Homologene: 69182
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Rbpms2
Name: RNA binding protein with multiple splicing 2
Synonyms: 2400008B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71973
VEGA: 9
Homologene: 49895
Kif13b
Name: kinesin family member 13B
Synonyms: GAKIN, N-3 kinesin, C130021D12Rik, 5330429L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16554
VEGA: 14
Homologene: 9073
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Casp3
Name: caspase 3
Synonyms: CPP32, Apopain, Yama, Caspase-3, A830040C14Rik, CC3, mldy, AC-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12367
HGNC: HGNC:1504
Homologene: 37912
Secisbp2l
Name: SECIS binding protein 2-like
Synonyms: 3110001I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70354
Homologene: 18923
Rnf181
Name: ring finger protein 181
Synonyms: 1700047F16Rik, 6230415N01Rik, 2500002L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66510
Homologene: 9537
Iars2
Name: isoleucine-tRNA synthetase 2, mitochondrial
Synonyms: 2010002H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381314
Homologene: 7118
Nr3c1
Name: nuclear receptor subfamily 3, group C, member 1
Synonyms: GR, Grl-1, Grl1, glucocorticoid receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14815
HGNC: HGNC:7978
Homologene: 30960
B3gnt3
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Synonyms: 2210008L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72297
Homologene: 113516
Zdhhc18
Name: zinc finger, DHHC domain containing 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 503610
Homologene: 110918
C2cd5
Name: C2 calcium-dependent domain containing 5
Synonyms: C030008B15Rik, CDP138, 5730419I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74741
Homologene: 8873
Klhl12
Name: kelch-like 12
Synonyms: C3ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240756
Homologene: 23317
Tbc1d1
Name: TBC1 domain family, member 1
Synonyms: 1110062G02Rik, Nob1, Nobq1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57915
Homologene: 56856
Kank4
Name: KN motif and ankyrin repeat domains 4
Synonyms: Ankrd38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242553
Homologene: 18244
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Tcf25
Name: transcription factor 25 (basic helix-loop-helix)
Synonyms: 1810041K11Rik, Nulp1, 1100001J13Rik, D8Ertd325e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66855
Homologene: 5701
Cul2
Name: cullin 2
Synonyms: 1300003D18Rik, 4932411N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71745
HGNC: HGNC:2552
Homologene: 2662
Gzf1
Name: GDNF-inducible zinc finger protein 1
Synonyms: 8430437G08Rik, Zfp336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74533
Homologene: 11200
C1ql3
Name: C1q-like 3
Synonyms: K100, 1110065A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227580
Homologene: 17701
Atp12a
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: HKalpha2, cHKA, Atp1al1, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 192113
VEGA: 14
Homologene: 68197
Cabp1
Name: calcium binding protein 1
Synonyms: caldendrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29867
HGNC: HGNC:1384
Homologene: 99790
Aff2
Name: AF4/FMR2 family, member 2
Synonyms: Ox19, Oxh, Fmr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14266
HGNC: HGNC:3776
Homologene: 1532
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Tea, Atrc2, Cat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11988
Homologene: 20659
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Ptprd
Name: protein tyrosine phosphatase, receptor type, D
Synonyms: 3000002J10Rik, B230219D21Rik, 1110002J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19266
HGNC: HGNC:9668
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Uba7
Name: ubiquitin-like modifier activating enzyme 7
Synonyms: 1300004C08Rik, Ube1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74153
Homologene: 2502
Cfap126
Name: cilia and flagella associated protein 126
Synonyms: Fltp, Flattop, 1700009P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75472
Homologene: 23682
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Acss3
Name: acyl-CoA synthetase short-chain family member 3
Synonyms: LOC380660, 8430416H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 380660
Homologene: 11587
Slc13a4
Name: solute carrier family 13 (sodium/sulfate symporters), member 4
Synonyms: SUT-1, SUT1, 9630060C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243755
Homologene: 69125
Ubxn8
Name: UBX domain protein 8
Synonyms: Rep8h, Rep-8, D0H8S2298E, Ubxd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108159
Homologene: 4149
Lgals12
Name: lectin, galactose binding, soluble 12
Synonyms: galectin-related inhibitor of proliferation, GRIP1, galectin-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56072
Homologene: 10462
Cd248
Name: CD248 antigen, endosialin
Synonyms: 2610111G01Rik, Tem1, Cd164l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 70445
VEGA: 19
Homologene: 10699
Spib
Name: Spi-B transcription factor (Spi-1/PU.1 related)
Synonyms: Spi-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 272382
Homologene: 79220
1700129C05Rik
Name: RIKEN cDNA 1700129C05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67932
Homologene: 130749
Olfr1471
Name: olfactory receptor 1471
Synonyms: GA_x6K02T2RE5P-3777626-3778570, MOR202-38, MOR202-47_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258231
Homologene: 79417
Olfr1279
Name: olfactory receptor 1279
Synonyms: GA_x6K02T2Q125-72357646-72358581, MOR245-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258388
Homologene: 121520
Cdh18
Name: cadherin 18
Synonyms: B230220E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320865
HGNC: HGNC:1757
Homologene: 55858
Olfr988
Name: olfactory receptor 988
Synonyms: GA_x6K02T2Q125-46830591-46829662, MOR203-5P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258166
Homologene: 103791
Olfr317
Name: olfactory receptor 317
Synonyms: GA_x6K02T2NKPP-680866-681849, MOR256-47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 257931
Homologene: 128382
Rnf133
Name: ring finger protein 133
Synonyms: Greul2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 386611
Homologene: 44549
Slc2a5
Name: solute carrier family 2 (facilitated glucose transporter), member 5
Synonyms: GLUT5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56485
Homologene: 74459
Pla2g4d
Name: phospholipase A2, group IVD
Synonyms: 2610311B01Rik, Pla2delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78390
Homologene: 52252
Prl3d1
Name: prolactin family 3, subfamily d, member 1
Synonyms: Pl-1, Pl1, prolactin-like 2, PL-Ia, Csh1, placental lactogen 1, mPL-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18775
Homologene: 137215
Krt9
Name: keratin 9
Synonyms: K9, Krt1-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107656
HGNC: HGNC:6447
Homologene: 138337
Zfp970
Name: zinc finger protein 970
Synonyms: Gm14420
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 628308
Homologene: 134324
Tesl2
Name: testin LIM domain protein like 2
Synonyms: Gm4985
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245376
Mbtps2
Name: membrane-bound transcription factor peptidase, site 2
Synonyms: 9630032G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 270669
Homologene: 9287
Cldn24
Name: claudin 24
Synonyms: Gm10107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100039801
Homologene: 136011
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 134,463,820 bp
  • C to T, chromosome 1 at 171,126,022 bp
  • A to T, chromosome 1 at 185,325,494 bp
  • C to T, chromosome 2 at 13,010,319 bp
  • A to G, chromosome 2 at 85,353,858 bp
  • A to G, chromosome 2 at 111,306,310 bp
  • T to C, chromosome 2 at 118,723,765 bp
  • T to C, chromosome 2 at 120,271,141 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • T to C, chromosome 2 at 148,683,936 bp
  • T to A, chromosome 2 at 155,212,339 bp
  • T to A, chromosome 2 at 177,474,821 bp
  • C to T, chromosome 3 at 64,559,718 bp
  • C to T, chromosome 4 at 76,107,424 bp
  • A to T, chromosome 4 at 98,779,226 bp
  • A to T, chromosome 4 at 133,633,077 bp
  • T to C, chromosome 4 at 150,139,990 bp
  • A to G, chromosome 5 at 64,284,800 bp
  • A to T, chromosome 5 at 110,719,091 bp
  • T to A, chromosome 5 at 115,176,225 bp
  • T to C, chromosome 6 at 23,649,175 bp
  • A to G, chromosome 6 at 35,280,483 bp
  • G to T, chromosome 6 at 72,361,484 bp
  • A to T, chromosome 6 at 143,036,316 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to G, chromosome 7 at 44,529,968 bp
  • A to G, chromosome 8 at 33,635,364 bp
  • G to T, chromosome 8 at 36,508,422 bp
  • T to C, chromosome 8 at 40,905,621 bp
  • G to T, chromosome 8 at 46,637,955 bp
  • G to T, chromosome 8 at 47,822,328 bp
  • A to G, chromosome 8 at 48,292,277 bp
  • A to T, chromosome 8 at 71,692,818 bp
  • A to G, chromosome 8 at 95,763,186 bp
  • A to G, chromosome 8 at 123,374,033 bp
  • A to G, chromosome 9 at 65,649,437 bp
  • A to G, chromosome 9 at 107,976,364 bp
  • A to T, chromosome 10 at 8,729,977 bp
  • G to A, chromosome 10 at 107,004,748 bp
  • T to C, chromosome 11 at 58,732,995 bp
  • A to T, chromosome 11 at 100,190,859 bp
  • T to C, chromosome 11 at 103,501,467 bp
  • G to T, chromosome 11 at 107,111,322 bp
  • T to C, chromosome 13 at 27,094,998 bp
  • T to C, chromosome 14 at 47,189,341 bp
  • A to G, chromosome 14 at 56,376,258 bp
  • G to A, chromosome 14 at 59,142,612 bp
  • C to T, chromosome 14 at 64,742,298 bp
  • A to G, chromosome 15 at 23,410,805 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to T, chromosome 18 at 3,399,876 bp
  • G to T, chromosome 18 at 39,486,751 bp
  • A to G, chromosome 19 at 5,068,126 bp
  • T to A, chromosome 19 at 7,606,765 bp
  • T to C, chromosome 19 at 13,445,185 bp
  • G to A, chromosome X at 23,958,711 bp
  • G to A, chromosome X at 69,834,803 bp
  • A to T, chromosome X at 157,559,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2253 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040253-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.