Strain Name:
C57BL/6J-MtgxR2285Btlr/Mmmh
Stock Number:
040284-MU
Citation ID:
RRID:MMRRC_040284-MU
Other Names:
R2285 (G1), C57BL/6J-MtgxR2285Btlr
Major Collection:

Strain Information

Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269132
Homologene: 22865
Aebp2
Name: AE binding protein 2
Synonyms: B230313N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11569
Homologene: 40690
Prr12
Name: proline rich 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233210
Homologene: 18957
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Bbs9
Name: Bardet-Biedl syndrome 9 (human)
Synonyms: EST 3159894, E130103I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319845
Homologene: 44480
C3
Name: complement component 3
Synonyms: complement factor 3, acylation stimulating protein, Plp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Cnpy4
Name: canopy FGF signaling regulator 4
Synonyms: 2610019P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66455
Homologene: 15196
Usp54
Name: ubiquitin specific peptidase 54
Synonyms: 4930429G18Rik, C030002J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 78787
Homologene: 90902
Clu
Name: clusterin
Synonyms: testosterone repressed prostate message-2, SP-40, complement lysis inhibitor, Sgp-2, ApoJ, Apolipoprotein J, Cli, Sugp-2, Sgp2, D14Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12759
HGNC: HGNC:2095
Homologene: 1382
Gp2
Name: glycoprotein 2 (zymogen granule membrane)
Synonyms: 2310037I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67133
HGNC: HGNC:4441
Homologene: 133790
Col28a1
Name: collagen, type XXVIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213945
Homologene: 66345
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Tubgcp6
Name: tubulin, gamma complex associated protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328580
Homologene: 32477
Naip6
Name: NLR family, apoptosis inhibitory protein 6
Synonyms: Naip-rs4A, Naip-rs4, Birc1f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17952
HGNC: HGNC:7634
Homologene: 113589
Abcg3
Name: ATP binding cassette subfamily G member 3
Synonyms: Mxr2, Abcp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27405
HGNC: HGNC:74
Homologene: 86845
Nxpe3
Name: neurexophilin and PC-esterase domain family, member 3
Synonyms: LOC208684, LOC385658, Fam55c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 385658
VEGA: 16
Homologene: 17030
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: 5830419M17Rik, gp115, EMILIN-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100952
Homologene: 5117
Tmc1
Name: transmembrane channel-like gene family 1
Synonyms: Bth, Beethoven, 4933416G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13409
VEGA: 19
Homologene: 23670
Or6c66b
Name: olfactory receptor family 6 subfamily C member 66B
Synonyms: GA_x6K02T2PULF-11219415-11220350, MOR108-2, Olfr792
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258150
Homologene: 133881
Kcnh3
Name: potassium voltage-gated channel, subfamily H (eag-related), member 3
Synonyms: ether a go-go like, Elk2, Melk2, C030044P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16512
VEGA: 15
HGNC: HGNC:6252
Homologene: 32150
Gfra4
Name: glial cell line derived neurotrophic factor family receptor alpha 4
Synonyms: GFR alpha-4, G630015H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14588
Homologene: 10565
Mipep
Name: mitochondrial intermediate peptidase
Synonyms: 5730405E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70478
VEGA: 14
HGNC: HGNC:7104
Homologene: 4337
Ppcs
Name: phosphopantothenoylcysteine synthetase
Synonyms: 6330579B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 106564
Homologene: 6173
Cpa5
Name: carboxypeptidase A5
Synonyms: 4930430M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74649
Homologene: 62246
Cibar2
Name: CBY1 interacting BAR domain containing 2
Synonyms: 1700120B06Rik, Fam92b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 436062
Homologene: 78513
Zfp51
Name: zinc finger protein 51
Synonyms: zfec12, Zfp-51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22709
VEGA: 17
Homologene: 134003
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 152,468,550 bp
  • C to T, chromosome 2 at 131,041,731 bp
  • T to A, chromosome 4 at 119,418,977 bp
  • G to A, chromosome 5 at 30,918,200 bp
  • A to G, chromosome 5 at 104,939,171 bp
  • A to G, chromosome 5 at 138,192,825 bp
  • A to T, chromosome 6 at 8,097,078 bp
  • G to T, chromosome 6 at 30,615,064 bp
  • T to C, chromosome 6 at 140,650,749 bp
  • T to C, chromosome 7 at 45,050,136 bp
  • C to T, chromosome 7 at 119,450,085 bp
  • T to C, chromosome 8 at 120,168,537 bp
  • G to T, chromosome 9 at 16,376,173 bp
  • T to G, chromosome 9 at 22,678,934 bp
  • A to G, chromosome 10 at 129,540,668 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • G to A, chromosome 14 at 20,561,178 bp
  • T to C, chromosome 14 at 60,787,394 bp
  • T to C, chromosome 14 at 65,007,997 bp
  • T to C, chromosome 14 at 65,980,959 bp
  • A to T, chromosome 15 at 89,122,474 bp
  • A to T, chromosome 15 at 99,241,992 bp
  • C to T, chromosome 16 at 55,866,225 bp
  • T to A, chromosome 17 at 21,463,875 bp
  • C to T, chromosome 17 at 57,215,105 bp
  • A to G, chromosome 19 at 20,789,799 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2285 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040284-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.