Strain Name:
C57BL/6J-MtgxR2289Btlr/Mmmh
Stock Number:
040288-MU
Citation ID:
RRID:MMRRC_040288-MU
Other Names:
R2289 (G1), C57BL/6J-MtgxR2289Btlr
Major Collection:

Gene Information

Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Styx
Name: serine/threonine/tyrosine interaction protein
Synonyms: STNS (alternatively spliced intron of Styx), 0610039A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 56291
VEGA: 14
Homologene: 41331
Phf14
Name: PHD finger protein 14
Synonyms: 5730446A07Rik, 1110001C23Rik, 4932409F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 75725
Homologene: 8775
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Dlg4
Name: discs large MAGUK scaffold protein 4
Synonyms: PSD-95, SAP90, Dlgh4, PSD95, SAP90A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 13385
HGNC: HGNC:2903
Homologene: 1047
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216860
Homologene: 13029
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: SKD2, N-ethylmaleimide sensitive factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Vrk1
Name: vaccinia related kinase 1
Synonyms: 51PK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 22367
VEGA: 12
Homologene: 2541
Thoc1
Name: THO complex 1
Synonyms: 3110002N20Rik, NMP-84
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225160
VEGA: 18
Homologene: 38012
Mcm5
Name: minichromosome maintenance complex component 5
Synonyms: mCD46, Cdc46, Mcmd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 17218
HGNC: HGNC:6948
Homologene: 4904
Lmtk2
Name: lemur tyrosine kinase 2
Synonyms: 2900041G10Rik, AATYK2, KPI-2, cprk, KPI2, A330101P12Rik, BREK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231876
Homologene: 8948
Camsap2
Name: calmodulin regulated spectrin-associated protein family, member 2
Synonyms: 4930541M15Rik, Camsap1l1, 1600013L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67886
Homologene: 18927
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: Tg(SMN2)89Ahmb, SMN2, 6330570A01Rik, mGlu7a receptor, E130018M02Rik, Gpr1g, mGluR7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Tsr1
Name: TSR1 20S rRNA accumulation
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 104662
Homologene: 5576
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 2310042E16Rik, Coh1, 1810042B05Rik, C330002D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Gm7964
Name: predicted gene 7964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 666170
Zranb1
Name: zinc finger, RAN-binding domain containing 1
Synonyms: 9330160G10Rik, D7Wsu87e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 360216
Homologene: 9728
Lman1l
Name: lectin, mannose-binding 1 like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235416
HGNC: HGNC:6632
Homologene: 11047
Vrk3
Name: vaccinia related kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101568
Homologene: 9509
Nle1
Name: notchless homolog 1
Synonyms: l11Jus1, Nle, notchless, l11Jus4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217011
Homologene: 5494
Zfp870
Name: zinc finger protein 870
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240066
VEGA: 17
Homologene: 48189
Hcrt
Name: hypocretin
Synonyms: orexin B, orexin A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 15171
HGNC: HGNC:4847
Homologene: 1166
Atp12a
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: Atp1al1, cHKA, HKalpha2, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 192113
VEGA: 14
Homologene: 68197
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola-2, Cola2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Fsd1l
Name: fibronectin type III and SPRY domain containing 1-like
Synonyms: Ccdc10, Fsd1nl, Csdufd1, A230072O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 319636
Homologene: 45825
Loxl2
Name: lysyl oxidase-like 2
Synonyms: 9430067E15Rik, 1110004B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 94352
HGNC: HGNC:6666
Homologene: 1742
2010300C02Rik
Name: RIKEN cDNA 2010300C02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 72097
Homologene: 19208
Dcaf10
Name: DDB1 and CUL4 associated factor 10
Synonyms: Wdr32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242418
Homologene: 32581
Rhobtb3
Name: Rho-related BTB domain containing 3
Synonyms: 4930503C18Rik, 1700040C17Rik, 2610033K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 73296
Homologene: 8932
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237711
Homologene: 104282
Mcrip1
Name: MAPK regulated corepressor interacting protein 1
Synonyms: Fam195b, BC003940
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192173
Homologene: 19571
Tmem163
Name: transmembrane protein 163
Synonyms: beta-actin-Cre, SV31, 2610024A01Rik, beta-actin:Cre, beta-actin-cre, Tg(ACTB-cre)2Mrt, creactin, actin-cre, TgN(ACTB-cre)2Mrt, beta-actin::Cre, Act-Cre, A-cre
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 72160
Homologene: 12798
Dixdc1
Name: DIX domain containing 1
Synonyms: 4930563F16Rik, Ccd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 330938
Homologene: 82369
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 627479
Homologene: 129606
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Zscan4b
Name: zinc finger and SCAN domain containing 4B
Synonyms: EG665780
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 665780
Homologene: 85986
Gm156
Name: predicted gene 156
Synonyms: LOC232415
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232415
Homologene: 16395
Snrpa1
Name: small nuclear ribonucleoprotein polypeptide A'
Synonyms: 1500015N06Rik, U2 snRNP-A'
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68981
Homologene: 2325
Pax8
Name: paired box 8
Synonyms: Pax-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18510
HGNC: HGNC:8622
Homologene: 2589
Samd13
Name: sterile alpha motif domain containing 13
Synonyms: LOC381481
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
Nqo1
Name: NAD(P)H dehydrogenase, quinone 1
Synonyms: Nmor1, Ox1, QR1, NQO1, Dia4, NAD(P)H dehydrogenase (quinone), Ox-1, NMO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18104
HGNC: HGNC:2874
Homologene: 695
Vash1
Name: vasohibin 1
Synonyms: G630009D10Rik, D930046M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238328
Homologene: 8941
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,612,261 bp
  • T to A, chromosome 1 at 127,495,740 bp
  • A to T, chromosome 1 at 136,276,766 bp
  • T to C, chromosome 2 at 24,440,740 bp
  • T to A, chromosome 2 at 71,818,529 bp
  • C to T, chromosome 3 at 146,662,691 bp
  • T to A, chromosome 4 at 45,359,816 bp
  • T to A, chromosome 4 at 53,696,931 bp
  • A to G, chromosome 5 at 108,801,682 bp
  • T to A, chromosome 5 at 144,176,106 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 12,047,846 bp
  • C to T, chromosome 6 at 104,569,028 bp
  • G to T, chromosome 6 at 110,646,348 bp
  • T to C, chromosome 6 at 129,768,177 bp
  • A to G, chromosome 7 at 10,901,862 bp
  • C to T, chromosome 7 at 44,775,442 bp
  • T to A, chromosome 7 at 66,063,838 bp
  • C to G, chromosome 7 at 83,756,769 bp
  • T to C, chromosome 7 at 132,950,039 bp
  • T to C, chromosome 8 at 75,117,702 bp
  • T to C, chromosome 8 at 107,392,998 bp
  • A to G, chromosome 9 at 50,683,872 bp
  • T to A, chromosome 9 at 57,613,658 bp
  • T to A, chromosome 11 at 29,785,653 bp
  • C to T, chromosome 11 at 69,906,715 bp
  • T to A, chromosome 11 at 70,026,926 bp
  • T to G, chromosome 11 at 74,899,285 bp
  • T to A, chromosome 11 at 82,903,053 bp
  • C to A, chromosome 11 at 100,761,919 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • T to C, chromosome 11 at 120,544,704 bp
  • G to C, chromosome 12 at 86,680,178 bp
  • G to A, chromosome 12 at 106,057,861 bp
  • A to C, chromosome 13 at 75,910,927 bp
  • A to G, chromosome 14 at 45,354,947 bp
  • G to A, chromosome 14 at 56,373,262 bp
  • G to A, chromosome 14 at 69,693,075 bp
  • A to C, chromosome 15 at 35,572,105 bp
  • A to G, chromosome 17 at 15,749,369 bp
  • A to T, chromosome 17 at 32,883,360 bp
  • T to C, chromosome 18 at 9,984,488 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2289 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040288-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.