Strain Name:
C57BL/6J-MtgxR2294Btlr/Mmmh
Stock Number:
040293-MU
Citation ID:
RRID:MMRRC_040293-MU
Other Names:
R2294 (G1), C57BL/6J-MtgxR2294Btlr
Major Collection:

Gene Information

Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7, alpha7 nicotinic receptor, alpha7-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11441
Homologene: 593
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 30953
Homologene: 130673
Trappc8
Name: trafficking protein particle complex 8
Synonyms: 5033403J15Rik, D030074E01Rik, Trs85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75964
VEGA: 18
Homologene: 40993
Slc38a2
Name: solute carrier family 38, member 2
Synonyms: 5033402L14Rik, SNAT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67760
VEGA: 15
Homologene: 23132
Chchd3
Name: coiled-coil-helix-coiled-coil-helix domain containing 3
Synonyms: 1700039J09Rik, 0610041L09Rik, Micos19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66075
Homologene: 9851
Lrp6
Name: low density lipoprotein receptor-related protein 6
Synonyms: skam26Jus, Cd, ska26, skax26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16974
HGNC: HGNC:6698
Homologene: 1747
G3bp2
Name: GTPase activating protein (SH3 domain) binding protein 2
Synonyms: G3BP, G3BP2, E430034L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23881
Homologene: 137244
Ripk1
Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms: Rinp, Rip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19766
Homologene: 2820
Rars
Name: arginyl-tRNA synthetase
Synonyms: 2610037E21Rik, 2610011N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104458
HGNC: HGNC:9870
Homologene: 68281
Ripor2
Name: RHO family interacting cell polarization regulator 2
Synonyms: 1700108N18Rik, E430013J17Rik, 6330500D04Rik, Fam65b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 193385
Homologene: 9284
Lypd8
Name: LY6/PLAUR domain containing 8
Synonyms: 2210415F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70163
Homologene: 52815
Ndufs1
Name: NADH:ubiquinone oxidoreductase core subunit S1
Synonyms: 9930026A05Rik, 5830412M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227197
HGNC: HGNC:7707
Homologene: 3670
Tgfb3
Name: transforming growth factor, beta 3
Synonyms: Tgfb-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21809
VEGA: 12
Homologene: 2433
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: eNOS, ecNOS, Nos-3, 2310065A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Fbxw18
Name: F-box and WD-40 domain protein 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546161
Homologene: 110776
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Dxo
Name: decapping exoribonuclease
Synonyms: Dom3z
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 112403
HGNC: HGNC:2992
Homologene: 38061
Tmem175
Name: transmembrane protein 175
Synonyms: 3010001K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72392
Homologene: 12461
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Tyro3
Name: TYRO3 protein tyrosine kinase 3
Synonyms: Sky, Dtk, Etk-2, Tif, Brt, Rse, Sky
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22174
Homologene: 4585
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: ORP-6, 1110062M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99031
Homologene: 101447
Yipf2
Name: Yip1 domain family, member 2
Synonyms: 1300010K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74766
VEGA: 9
Homologene: 23430
Itgbl1
Name: integrin, beta-like 1
Synonyms: with EGF-like repeat domains, B930011D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223272
HGNC: HGNC:6164
Homologene: 3519
Adcy6
Name: adenylate cyclase 6
Synonyms: AC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11512
VEGA: 15
HGNC: HGNC:237
Homologene: 22400
Slc6a21
Name: solute carrier family 6 member 21
Synonyms: 1700039E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76713
Homologene: 66347
Luzp2
Name: leucine zipper protein 2
Synonyms: 9330154K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233271
Homologene: 45618
Timd4
Name: T cell immunoglobulin and mucin domain containing 4
Synonyms: B430010N18Rik, Tim4, TIM-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 276891
Homologene: 51381
Tmem232
Name: transmembrane protein 232
Synonyms: LOC381107, E130009J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381107
VEGA: 17
Homologene: 54378
Or1e33
Name: olfactory receptor family 1 subfamily E member 33
Synonyms: GA_x6K02T2P1NL-4004140-4003208, MOR135-7, Olfr393
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259010
Homologene: 79335
Pik3c2b
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Synonyms: PI3K-C2beta, C330011J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240752
HGNC: HGNC:8972
Homologene: 20582
Prl7b1
Name: prolactin family 7, subfamily b, member 1
Synonyms: PLP-N, 1600014J19Rik, Prlpn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75596
HGNC: HGNC:9445
Homologene: 17809
Prelid3a
Name: PRELI domain containing 3A
Synonyms: A230074A19Rik, Slmo1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225655
VEGA: 18
Homologene: 90953
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 63,160,996 bp
  • C to A, chromosome 1 at 133,066,775 bp
  • A to T, chromosome 2 at 76,577,079 bp
  • T to C, chromosome 2 at 119,805,645 bp
  • T to A, chromosome 3 at 68,625,799 bp
  • T to A, chromosome 5 at 24,364,857 bp
  • G to A, chromosome 5 at 92,058,028 bp
  • T to C, chromosome 5 at 108,638,659 bp
  • T to C, chromosome 6 at 32,852,187 bp
  • A to G, chromosome 6 at 134,457,742 bp
  • G to A, chromosome 7 at 45,280,528 bp
  • T to C, chromosome 7 at 55,172,190 bp
  • A to T, chromosome 7 at 63,110,424 bp
  • T to C, chromosome 8 at 110,299,959 bp
  • C to T, chromosome 9 at 21,589,881 bp
  • C to T, chromosome 9 at 108,113,067 bp
  • A to G, chromosome 9 at 109,676,797 bp
  • C to T, chromosome 10 at 125,966,494 bp
  • C to A, chromosome 11 at 35,817,536 bp
  • T to C, chromosome 11 at 46,843,113 bp
  • A to T, chromosome 11 at 58,386,854 bp
  • A to G, chromosome 11 at 73,847,486 bp
  • T to C, chromosome 12 at 86,069,910 bp
  • A to T, chromosome 13 at 24,723,803 bp
  • A to T, chromosome 13 at 27,602,871 bp
  • A to G, chromosome 13 at 34,017,008 bp
  • T to C, chromosome 14 at 123,840,700 bp
  • C to T, chromosome 15 at 44,479,607 bp
  • C to A, chromosome 15 at 96,691,762 bp
  • C to T, chromosome 15 at 98,597,441 bp
  • G to A, chromosome 17 at 34,838,986 bp
  • A to T, chromosome 17 at 65,450,441 bp
  • G to T, chromosome 18 at 20,866,154 bp
  • C to T, chromosome 18 at 67,472,871 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040293-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.