Strain Name:
C57BL/6J-MtgxR2301Btlr/Mmmh
Stock Number:
040300-MU
Citation ID:
RRID:MMRRC_040300-MU
Other Names:
R2301 (G1), C57BL/6J-MtgxR2301Btlr
Major Collection:

Gene Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Secisbp2
Name: SECIS binding protein 2
Synonyms: 2210413N07Rik, SBP2, 2810012K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75420
Homologene: 11415
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76199
Homologene: 25256
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Apc1, 2610021O03Rik, Mcpr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17222
Homologene: 7414
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, H3a, sirm, Sh3bp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20402
Homologene: 40787
Fam222b
Name: family with sequence similarity 222, member B
Synonyms: BC017647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216971
Homologene: 10054
Klf10
Name: Kruppel-like factor 10
Synonyms: mGIF, Gdnfif, Egral, Tieg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21847
VEGA: 15
Homologene: 4135
Lyn
Name: LYN proto-oncogene, Src family tyrosine kinase
Synonyms: Hck-2, Yamaguchi sarcoma viral (v-yes-1) oncogene homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17096
HGNC: HGNC:6735
Homologene: 55649
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Dpp9
Name: dipeptidylpeptidase 9
Synonyms: DPRP2, 6430584G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224897
VEGA: 17
Homologene: 16385
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Tmem139
Name: transmembrane protein 139
Synonyms: A930027H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 109218
VEGA: 6
Homologene: 51920
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
F5
Name: coagulation factor V
Synonyms: Cf-5, Cf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Catsper1
Name: cation channel, sperm associated 1
Synonyms: KSper
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225865
VEGA: 19
Homologene: 14207
Pcdhac1
Name: protocadherin alpha subfamily C, 1
Synonyms: CNRc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 353236
HGNC: HGNC:8676
Homologene: 49561
Pde5a
Name: phosphodiesterase 5A, cGMP-specific
Synonyms: PDE5A1, Pde5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242202
HGNC: HGNC:8784
Homologene: 842
Olfr1301
Name: olfactory receptor 1301
Synonyms: GA_x6K02T2Q125-72805651-72806589, MOR248-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258889
Homologene: 74057
Clrn1
Name: clarin 1
Synonyms: USH3, A130002D11Rik, clarin-1, Ush3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229320
Homologene: 17738
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195046
Homologene: 133820
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Gm16380
Name: predicted pseudogene 16380
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100038996
VEGA: 9
Ogfod1
Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
Synonyms: 4930415J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270086
Homologene: 41238
Traf5
Name: TNF receptor-associated factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22033
Homologene: 27079
Gtf2a1l
Name: general transcription factor IIA, 1-like
Synonyms: 1700011N16Rik, Alf, Gtf2a1lf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71828
Homologene: 86732
Slco5a1
Name: solute carrier organic anion transporter family, member 5A1
Synonyms: A630033C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240726
Homologene: 57135
Tti2
Name: TELO2 interacting protein 2
Synonyms: BC019943
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234138
Homologene: 11836
Chst13
Name: carbohydrate sulfotransferase 13
Synonyms: Chst13, 1110067M19Rik, C4ST-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71797
Homologene: 44360
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 12,879,262 bp
  • C to T, chromosome 1 at 164,161,911 bp
  • T to C, chromosome 1 at 191,997,965 bp
  • T to A, chromosome 2 at 111,754,276 bp
  • T to C, chromosome 2 at 120,535,650 bp
  • A to G, chromosome 2 at 128,642,726 bp
  • T to G, chromosome 3 at 58,846,352 bp
  • T to A, chromosome 3 at 122,843,030 bp
  • G to A, chromosome 4 at 3,780,959 bp
  • T to A, chromosome 4 at 138,172,659 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to A, chromosome 5 at 118,593,447 bp
  • T to A, chromosome 5 at 121,353,537 bp
  • G to T, chromosome 6 at 42,263,430 bp
  • A to T, chromosome 6 at 90,318,289 bp
  • T to A, chromosome 7 at 105,705,399 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • T to C, chromosome 8 at 31,155,795 bp
  • C to T, chromosome 8 at 94,037,368 bp
  • A to G, chromosome 9 at 53,884,200 bp
  • T to C, chromosome 11 at 71,106,101 bp
  • T to C, chromosome 11 at 78,154,543 bp
  • T to C, chromosome 12 at 119,202,455 bp
  • T to C, chromosome 13 at 51,654,372 bp
  • T to C, chromosome 13 at 96,484,144 bp
  • A to G, chromosome 13 at 96,484,415 bp
  • A to G, chromosome 15 at 38,297,082 bp
  • A to G, chromosome 15 at 47,731,998 bp
  • A to G, chromosome 17 at 24,574,612 bp
  • C to T, chromosome 17 at 56,194,973 bp
  • G to T, chromosome 17 at 88,711,472 bp
  • A to G, chromosome 18 at 37,156,183 bp
  • G to A, chromosome 19 at 4,734,138 bp
  • A to G, chromosome 19 at 5,340,398 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2301 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040300-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.