Strain Name:
C57BL/6J-MtgxR2304Btlr/Mmmh
Stock Number:
040303-MU
Citation ID:
RRID:MMRRC_040303-MU
Other Names:
R2304 (G1), C57BL/6J-MtgxR2304Btlr
Major Collection:

Gene Information

Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 73658
Homologene: 41530
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: TRIP230, 2610511G22Rik, GMAP-210, 3110031G15Rik, 6030460N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 109181
Homologene: 20897
Grid2ip
Name: glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
Synonyms: delphilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 170935
Homologene: 15781
Xpot
Name: exportin, tRNA (nuclear export receptor for tRNAs)
Synonyms: C79645, 1110004L07Rik, EXPORTIN-T
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73192
VEGA: 10
Homologene: 38291
Ccdc187
Name: coiled-coil domain containing 187
Synonyms: 4932418E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329366
Homologene: 77630
Lifr
Name: LIF receptor alpha
Synonyms: soluble differentiation-stimulating factor receptor, A230075M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 16880
VEGA: 15
HGNC: HGNC:6597
Homologene: 1735
Rbl1
Name: RB transcriptional corepressor like 1
Synonyms: p107, retinoblastoma-like 1 (p107)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19650
HGNC: HGNC:9893
Homologene: 2172
Sp8
Name: trans-acting transcription factor 8
Synonyms: mBtd, D930049B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 320145
VEGA: 12
Homologene: 18548
Tns2
Name: tensin 2
Synonyms: Tenc1, nph, nep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 209039
VEGA: 15
Homologene: 37077
Dcdc5
Name: doublecortin domain containing 5
Synonyms: EG436559, 4732421G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329482
Homologene: 79722
Slc13a5
Name: solute carrier family 13 (sodium-dependent citrate transporter), member 5
Synonyms: Indy, NaC2/NaCT, mINDY, Nact
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237831
Homologene: 21941
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Serac1
Name: serine active site containing 1
Synonyms: D17Ertd141e, 4930511N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 321007
Homologene: 41900
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Mcrip1
Name: MAPK regulated corepressor interacting protein 1
Synonyms: BC003940, Fam195b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192173
Homologene: 19571
Ptpru
Name: protein tyrosine phosphatase, receptor type, U
Synonyms: Ptprl, RPTPlambda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 19273
HGNC: HGNC:9683
Homologene: 4168
Vmn1r184
Name: vomeronasal 1 receptor, 184
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100312477
Homologene: 74353
Togaram1
Name: TOG array regulator of axonemal microtubules 1
Synonyms: Fam179b, A430041B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 328108
VEGA: 12
Homologene: 15025
Dvl1
Name: dishevelled segment polarity protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13542
HGNC: HGNC:3084
Homologene: 20926
Olfr1135
Name: olfactory receptor 1135
Synonyms: MOR177-2, GA_x6K02T2Q125-49177531-49176599
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258654
Homologene: 17331
Slco5a1
Name: solute carrier organic anion transporter family, member 5A1
Synonyms: A630033C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240726
Homologene: 57135
Zfp786
Name: zinc finger protein 786
Synonyms: A730012O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 330301
Homologene: 51849
Prss53
Name: protease, serine 53
Synonyms: BC039632
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 330657
Homologene: 80097
Rnaseh2b
Name: ribonuclease H2, subunit B
Synonyms: Dleu8, 2610207P08Rik, 1110019N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 67153
Homologene: 41572
Gkn3
Name: gastrokine 3
Synonyms: 1190003M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68888
Homologene: 87422
Erg28
Name: ergosterol biosynthesis 28
Synonyms: 0610007P14Rik, ORF11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 58520
HGNC: HGNC:1187
Homologene: 38284
Sh2d6
Name: SH2 domain containing 6
Synonyms: 4933424C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 71130
Homologene: 65986
Gm5449
Name: predicted pseudogene 5449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 432760
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 12,879,262 bp
  • T to C, chromosome 2 at 26,281,017 bp
  • T to A, chromosome 2 at 87,671,894 bp
  • T to A, chromosome 2 at 106,336,143 bp
  • T to C, chromosome 2 at 157,147,631 bp
  • A to T, chromosome 4 at 131,772,568 bp
  • C to T, chromosome 4 at 155,855,584 bp
  • T to A, chromosome 5 at 8,092,366 bp
  • T to C, chromosome 5 at 143,387,840 bp
  • A to G, chromosome 6 at 47,820,699 bp
  • T to C, chromosome 6 at 72,520,559 bp
  • C to T, chromosome 6 at 87,383,525 bp
  • T to C, chromosome 7 at 26,267,125 bp
  • T to C, chromosome 7 at 46,251,398 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • T to C, chromosome 7 at 127,888,307 bp
  • T to C, chromosome 10 at 121,611,583 bp
  • A to G, chromosome 11 at 72,259,039 bp
  • A to T, chromosome 11 at 120,544,693 bp
  • G to T, chromosome 12 at 64,976,856 bp
  • A to G, chromosome 12 at 85,816,163 bp
  • A to T, chromosome 12 at 101,898,977 bp
  • G to T, chromosome 12 at 118,848,569 bp
  • G to T, chromosome 13 at 53,525,863 bp
  • T to C, chromosome 14 at 62,361,389 bp
  • T to C, chromosome 15 at 7,186,862 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • G to A, chromosome 17 at 6,044,253 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2304 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040303-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.