Strain Name:
C57BL/6J-MtgxR2305Btlr/Mmmh
Stock Number:
040304-MU
Citation ID:
RRID:MMRRC_040304-MU
Other Names:
R2305 (G1), C57BL/6J-MtgxR2305Btlr
Major Collection:

Gene Information

Acvr1c
Name: activin A receptor, type IC
Synonyms: ALK7, Alk-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269275
Homologene: 26724
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20562
Homologene: 2302
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: protein kinase U-alpha, PKUalpha, 4933403M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 24086
Homologene: 4993
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Exo1
Name: exonuclease 1
Synonyms: Msa, 5730442G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26909
HGNC: HGNC:3511
Homologene: 31352
Med25
Name: mediator complex subunit 25
Synonyms: ESTM2, 2610529E18Rik, 2610034E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75613
Homologene: 12614
Phkb
Name: phosphorylase kinase beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102093
HGNC: HGNC:8927
Homologene: 247
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Cep135
Name: centrosomal protein 135
Synonyms: LOC381644, Cep4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381644
Homologene: 45709
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, Rasl2-8, lipopolysaccharide response
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21898
Homologene: 41317
Pirb
Name: paired Ig-like receptor B
Synonyms: Gp91, Lilrb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18733
HGNC: HGNC:6607
Homologene: 134028
Polr2b
Name: polymerase (RNA) II (DNA directed) polypeptide B
Synonyms: RPB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231329
HGNC: HGNC:9188
Homologene: 722
Casp8ap2
Name: caspase 8 associated protein 2
Synonyms: D4Ertd659e, FLASH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26885
HGNC: HGNC:1510
Homologene: 8066
Tmtc1
Name: transmembrane and tetratricopeptide repeat containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387314
Homologene: 65299
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1134Clo, b2b1537Clo, b2b1565Clo, b2b1154Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230500
Homologene: 19952
Tubd1
Name: tubulin, delta 1
Synonyms: 4930550G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56427
Homologene: 9443
Adamts7
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
Synonyms: ADAM-TS7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108153
HGNC: HGNC:223
Homologene: 22803
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 1110004O12Rik, 9130019P20Rik, 4933432B13Rik, A530027J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108857
Homologene: 87006
Olfr1335
Name: olfactory receptor 1335
Synonyms: GA_x6K02T2QD9B-18723799-18724749, GA_x6K02T2QD9B-18726774-18727577, MOR259-5, MOR259-12, Olfr1335, Olfr1334-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435804
Homologene: 138635
Ccdc105
Name: coiled-coil domain containing 105
Synonyms: 4931413A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70976
VEGA: 10
Homologene: 18299
Ofcc1
Name: orofacial cleft 1 candidate 1
Synonyms: Opo, ojoplano
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218165
VEGA: 13
Homologene: 125376
Lgals12
Name: lectin, galactose binding, soluble 12
Synonyms: galectin-related inhibitor of proliferation, GRIP1, galectin-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56072
Homologene: 10462
Cyp3a57
Name: cytochrome P450, family 3, subfamily a, polypeptide 57
Synonyms: EG622127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622127
Homologene: 135775
Pus1
Name: pseudouridine synthase 1
Synonyms: MPUS1, mPus1p, A730013B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56361
Homologene: 5931
Vmn1r64
Name: vomeronasal 1 receptor 64
Synonyms: V1rd11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404285
Homologene: 41799
Rasgrf1
Name: RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms: CDC25Mm, CDC25, Grfbeta, Grf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19417
HGNC: HGNC:9875
Homologene: 74972
Tmem214
Name: transmembrane protein 214
Synonyms: 4921530J21Rik, 1110039B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68796
Homologene: 9801
Ugt3a2
Name: UDP glycosyltransferases 3 family, polypeptide A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223337
Homologene: 122787
Olfr1436
Name: olfactory receptor 1436
Synonyms: GA_x6K02T2RE5P-2634596-2633658, MOR214-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258682
Homologene: 83129
Serpina3a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3A
Synonyms: 4933406L18Rik, antitrypsin, alpha-1 antiproteinase,
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74069
HGNC: HGNC:16
Krt9
Name: keratin 9
Synonyms: K9, Krt1-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107656
HGNC: HGNC:6447
Homologene: 138337
Hps4
Name: HPS4, biogenesis of lysosomal organelles complex 3 subunit 2
Synonyms: C130020P05Rik, BLOC-3, 2010205O06Rik, Hermansky-Pudlak syndrome 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 192232
Homologene: 11123
Defb21
Name: defensin beta 21
Synonyms: LOC228782, 4930525K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 403172
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 30,805,475 bp
  • C to T, chromosome 1 at 175,888,761 bp
  • C to A, chromosome 2 at 58,281,699 bp
  • T to A, chromosome 2 at 152,574,871 bp
  • A to T, chromosome 4 at 32,646,411 bp
  • A to G, chromosome 4 at 66,840,101 bp
  • A to G, chromosome 4 at 99,831,481 bp
  • T to G, chromosome 4 at 118,808,861 bp
  • A to G, chromosome 5 at 30,875,777 bp
  • T to C, chromosome 5 at 76,595,389 bp
  • T to A, chromosome 5 at 77,320,437 bp
  • C to A, chromosome 5 at 110,774,960 bp
  • A to G, chromosome 5 at 112,346,661 bp
  • A to G, chromosome 5 at 145,381,280 bp
  • T to C, chromosome 6 at 148,244,697 bp
  • G to A, chromosome 7 at 3,712,991 bp
  • A to G, chromosome 7 at 5,884,536 bp
  • C to A, chromosome 7 at 44,885,890 bp
  • A to G, chromosome 8 at 86,043,802 bp
  • A to T, chromosome 9 at 90,020,300 bp
  • A to G, chromosome 9 at 90,180,711 bp
  • C to T, chromosome 10 at 5,047,573 bp
  • T to C, chromosome 10 at 78,748,502 bp
  • T to A, chromosome 11 at 86,555,191 bp
  • T to A, chromosome 11 at 100,193,116 bp
  • T to G, chromosome 11 at 105,241,591 bp
  • C to A, chromosome 12 at 104,116,528 bp
  • T to C, chromosome 13 at 40,263,658 bp
  • C to A, chromosome 15 at 9,351,117 bp
  • A to T, chromosome 15 at 28,387,767 bp
  • A to T, chromosome 16 at 37,062,337 bp
  • T to C, chromosome 18 at 36,642,926 bp
  • A to G, chromosome 19 at 7,603,956 bp
  • T to C, chromosome 19 at 12,299,087 bp
  • G to A, chromosome 19 at 41,611,016 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2305 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040304-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.